Detailed information    

insolico Bioinformatically predicted

Overview


Name   pilA   Type   Machinery gene
Locus tag   PPW95_RS11310 Genome accession   NZ_CP117039
Coordinates   617999..618427 (-) Length   142 a.a.
NCBI ID   WP_009698754.1    Uniprot ID   A0A8B3DN92
Organism   Vibrio parahaemolyticus strain VP220218     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context


Location: 612999..623427
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PPW95_RS11290 (PPW95_11290) coaE 613517..614131 (-) 615 WP_005480887.1 dephospho-CoA kinase -
  PPW95_RS11295 (PPW95_11295) pilD 614132..615001 (-) 870 WP_031855539.1 prepilin peptidase Machinery gene
  PPW95_RS11300 (PPW95_11300) pilC 615066..616289 (-) 1224 WP_023585959.1 type II secretion system F family protein Machinery gene
  PPW95_RS11305 (PPW95_11305) pilB 616314..617999 (-) 1686 WP_031855538.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PPW95_RS11310 (PPW95_11310) pilA 617999..618427 (-) 429 WP_009698754.1 type IV pilin protein Machinery gene
  PPW95_RS11315 (PPW95_11315) nadC 618692..619579 (-) 888 WP_015297249.1 carboxylating nicotinate-nucleotide diphosphorylase -
  PPW95_RS11320 (PPW95_11320) ampD 619672..620223 (+) 552 WP_025637088.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  PPW95_RS11325 (PPW95_11325) pdhR 620629..621396 (+) 768 WP_005462576.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence


Protein


Download         Length: 142 a.a.        Molecular weight: 14524.57 Da        Isoelectric Point: 5.7554

>NTDB_id=782187 PPW95_RS11310 WP_009698754.1 617999..618427(-) (pilA) [Vibrio parahaemolyticus strain VP220218]
MKTNKQKKQQGFTLIELMIVVAVIGVLAAIAIPQYQNYVKKSEAAAGLATLRSLTTNIDTFIADTGTFPADADAPTLGAA
VDMNKLGTISFADSGASGATATFTFDGTASALASTDTVVLAKDATTGLWTCSHSTGVTLKGC

Nucleotide


Download         Length: 429 bp        

>NTDB_id=782187 PPW95_RS11310 WP_009698754.1 617999..618427(-) (pilA) [Vibrio parahaemolyticus strain VP220218]
ATGAAAACGAATAAACAAAAAAAGCAGCAGGGTTTTACTCTAATTGAATTGATGATCGTGGTAGCGGTTATTGGTGTTTT
AGCAGCGATTGCTATTCCACAGTATCAGAATTATGTAAAAAAATCAGAGGCTGCAGCTGGGCTTGCAACTCTTCGTTCAC
TAACGACAAATATTGATACCTTCATTGCTGATACTGGCACCTTTCCTGCTGATGCCGACGCACCCACGTTAGGTGCTGCT
GTTGATATGAACAAACTTGGAACAATTTCTTTTGCAGATAGTGGTGCATCGGGAGCGACAGCAACGTTTACTTTTGATGG
TACAGCTAGCGCACTAGCTTCTACTGATACCGTTGTCCTTGCGAAGGATGCAACTACCGGACTTTGGACCTGCTCTCATA
GTACTGGCGTAACTCTAAAAGGCTGTTAA


Secondary structure


Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure


Source ID Structure
  AlphaFold DB A0A8B3DN92

Transmembrane helices


Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.



Visualization of predicted probability:


Similar proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

51.299

100

0.556

  pilA Vibrio cholerae strain A1552

51.299

100

0.556

  pilA Vibrio cholerae C6706

51.299

100

0.556

  pilA Vibrio parahaemolyticus RIMD 2210633

48.951

100

0.493

  pilA Pseudomonas aeruginosa PAK

45.27

100

0.472

  pilA Acinetobacter baumannii strain A118

35.294

100

0.38