Detailed information NTG0779327

Bioinformatically predicted

Overview

• Name: dprA
• Type: Machinery gene
• Locus tag: POF63_RS05280
• Genome accession: NZ_CP116708
• Coordinates: 1005289..1006131 (-)
• Length: 280 a.a.
• NCBI ID: WP_001015249.1
• Uniprot ID: -
• Organism: Streptococcus agalactiae strain CNCTC 10/84 sCas9
• Function: ssDNA binding; loading RecA onto ssDNA (predicted from homology) DNA processing

Genomic Context

Location: 1000289..1011131

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
POF63_RS05270 (POF63_05270)	-	1002236..1002937 (-)	702	WP_001146344.1	ABC transporter ATP-binding protein	-
POF63_RS05275 (POF63_05275)	topA	1003074..1005194 (-)	2121	WP_000246611.1	type I DNA topoisomerase	-
POF63_RS05280 (POF63_05280)	dprA	1005289..1006131 (-)	843	WP_001015249.1	DNA-processing protein DprA	Machinery gene
POF63_RS05285 (POF63_05285)	-	1006270..1007298 (-)	1029	WP_000162771.1	siderophore ABC transporter substrate-binding protein	-
POF63_RS05290 (POF63_05290)	-	1007360..1008121 (-)	762	WP_000614750.1	ATP-binding cassette domain-containing protein	-
POF63_RS05295 (POF63_05295)	-	1008118..1009092 (-)	975	WP_000588581.1	iron chelate uptake ABC transporter family permease subunit	-
POF63_RS05300 (POF63_05300)	ceuB	1009089..1010051 (-)	963	WP_000735583.1	iron chelate uptake ABC transporter family permease subunit	Machinery gene
POF63_RS05305 (POF63_05305)	-	1010291..1010839 (-)	549	WP_000136503.1	sugar O-acetyltransferase	-

Sequence

Protein

• Length: 280 a.a.
• Molecular weight: 31257.30 Da
• Isoelectric Point: 9.3800

>NTDB_id=779327 POF63_RS05280 WP_001015249.1 1005289..1006131(-) (dprA) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
MNHFELFKLKKAGLTNLNINNIINYLKKNSLTSLSVRNMAVVSKCKNPTFFIENYKQLDLKKLRQEFKKFPVLSILDSNY
PLELKEIYNPPVLLFYQGNIELLSKPKLAVVGARQASQIGCQSVKKIIKETNNQFVIVSGLARGIDTAAHVSALKNGGSS
IAVIGSGLDVYYPTENKKLQEYMSYNHLVLSEYFSGEQPLKFHFPERNRIIAGLCQGIVVAEAKMRSGSLITCERALEEG
REVFAIPGNIIDGKSDGCHHLIQEGAKCIISGKDILSEYQ

Nucleotide

• Length: 843 bp

>NTDB_id=779327 POF63_RS05280 WP_001015249.1 1005289..1006131(-) (dprA) [Streptococcus agalactiae strain CNCTC 10/84 sCas9]
ATGAATCATTTTGAACTATTTAAGCTTAAAAAAGCTGGACTAACAAATCTTAATATCAACAATATTATCAACTACCTTAA
AAAGAATAGTTTAACTTCTCTATCTGTTCGCAATATGGCCGTAGTGTCAAAATGTAAAAACCCTACTTTCTTTATAGAAA
ATTATAAACAGCTAGACCTTAAAAAACTTCGACAAGAATTCAAAAAATTTCCAGTTCTATCGATTTTGGATTCTAACTAT
CCTTTAGAGTTAAAAGAAATATATAATCCACCAGTTCTACTTTTTTATCAGGGTAATATTGAACTTCTATCTAAACCTAA
ATTAGCTGTAGTGGGAGCAAGACAGGCATCTCAGATAGGTTGTCAGTCTGTTAAAAAGATTATCAAGGAGACTAACAATC
AATTTGTTATCGTAAGTGGTTTAGCGCGTGGCATTGATACAGCAGCACATGTTAGTGCTTTAAAAAATGGAGGCAGTAGT
ATAGCTGTTATCGGGAGTGGTTTAGATGTTTATTATCCAACGGAGAATAAGAAACTTCAAGAATATATGTCATATAATCA
TCTCGTATTATCAGAATATTTTTCCGGAGAACAACCCTTGAAATTTCATTTCCCCGAACGTAACCGTATTATTGCAGGGC
TGTGTCAAGGTATTGTGGTTGCCGAAGCTAAGATGAGATCTGGAAGTTTAATTACCTGTGAAAGAGCATTAGAAGAGGGA
CGAGAAGTTTTTGCTATTCCCGGAAATATTATCGATGGCAAATCAGATGGATGCCACCATCTTATCCAAGAAGGAGCTAA
ATGCATTATTTCAGGAAAAGATATCCTTTCTGAATATCAGTAG

Domains

Predicted by InterproScan.

• DNA recombination-mediator protein A (DNA_processg_A) (69-270)

• DNA processing protein A sterile alpha motif domain (SAM_DprA) (1-63)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
dprA	Streptococcus mutans UA159	70.251	99.643	0.7
dprA/cilB/dalA	Streptococcus mitis SK321	62.5	100	0.625
dprA/cilB/dalA	Streptococcus pneumoniae Rx1	61.786	100	0.618
dprA/cilB/dalA	Streptococcus pneumoniae D39	61.786	100	0.618
dprA/cilB/dalA	Streptococcus pneumoniae R6	61.786	100	0.618
dprA/cilB/dalA	Streptococcus pneumoniae TIGR4	61.786	100	0.618
dprA/cilB/dalA	Streptococcus mitis NCTC 12261	61.429	100	0.614
dprA	Lactococcus lactis subsp. cremoris KW2	61.29	99.643	0.611
dprA	Latilactobacillus sakei subsp. sakei 23K	42.231	89.643	0.379
dprA	Haemophilus influenzae Rd KW20	37.132	97.143	0.361