Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PJW05_RS24605 Genome accession   NZ_CP116304
Coordinates   5336771..5338867 (-) Length   698 a.a.
NCBI ID   WP_271409542.1    Uniprot ID   -
Organism   Pseudomonas sp. Q1-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5331771..5343867
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJW05_RS24590 - 5333436..5335082 (-) 1647 WP_271409539.1 AAA family ATPase -
  PJW05_RS24595 aroB 5335092..5336195 (-) 1104 WP_271409540.1 3-dehydroquinate synthase -
  PJW05_RS24600 aroK 5336247..5336765 (-) 519 WP_271409541.1 shikimate kinase AroK -
  PJW05_RS24605 pilQ 5336771..5338867 (-) 2097 WP_271409542.1 type IV pilus secretin PilQ Machinery gene
  PJW05_RS24610 pilP 5338922..5339455 (-) 534 WP_271409543.1 type 4a pilus biogenesis lipoprotein PilP -
  PJW05_RS24615 pilO 5339452..5340075 (-) 624 WP_271409544.1 type 4a pilus biogenesis protein PilO -
  PJW05_RS24620 pilN 5340072..5340686 (-) 615 WP_271409545.1 type 4a pilus biogenesis protein PilN -
  PJW05_RS24625 pilM 5340686..5341750 (-) 1065 WP_271409546.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 74873.14 Da        Isoelectric Point: 4.9795

>NTDB_id=776519 PJW05_RS24605 WP_271409542.1 5336771..5338867(-) (pilQ) [Pseudomonas sp. Q1-7]
MKSSLSRLGITLLAALLSPALLAANLQALDVAALPGDRVELKLAFDEPVVAPRGYTIEQPARIALDLPGVSSKLATKNRE
LGVGNARSVTVVEAKDRTRLIINLSTLVPYSTRVEGRNLYVLVGDSVASAPRTVAAAPAPTAAPLSVSQPVKSYARAARA
IENIDFQRGDQGEGNVVITLSDPSVAPDIQEQGGRIRLDFSKTQLPESLRVRLDVKDFATPVQFVSASGSGANASISIEP
TGFYDYLAYQTDNKLTVSIKPLTQDDVEKRKAERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRL
QNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIAELAPLRRELIQVNYAKAADIAKLFQSVTSG
DPSAVGTDERGSITVDDRTNSIIAYQTQDKLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKALGVNWRGASIGDNNFV
VGGGGAGRPAAGQAGNVNLANAPFVDLGASGATSSIGIGFITDNTILDLELSAMEKTGNGEIVSQPKVVTSDKETAKILK
GAEIPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFANALNGVPPINKNEVNAKVLVNDGETIVI
GGVFSNTQTKAVDKVPFLGDLPFLGRMFRRDVVQDKKAELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=776519 PJW05_RS24605 WP_271409542.1 5336771..5338867(-) (pilQ) [Pseudomonas sp. Q1-7]
ATGAAAAGCTCGCTATCGCGCCTTGGTATCACGCTATTGGCCGCCCTGCTCTCGCCCGCCCTGCTGGCGGCGAACCTGCA
GGCACTGGATGTCGCGGCTCTCCCGGGCGACAGGGTTGAACTCAAGCTGGCGTTCGACGAGCCGGTGGTCGCCCCCCGCG
GCTACACCATCGAGCAGCCTGCGCGTATCGCCCTGGACCTGCCGGGTGTGTCCAGCAAGTTGGCGACCAAGAACCGCGAG
CTGGGCGTGGGCAATGCCCGCAGCGTGACTGTGGTCGAGGCCAAGGATCGCACCCGCCTGATCATCAACCTCTCCACCCT
CGTGCCCTACAGCACGCGCGTGGAGGGTCGTAACCTCTATGTGCTGGTAGGCGACTCGGTTGCCTCGGCGCCGCGCACCG
TGGCTGCCGCACCGGCGCCCACTGCAGCTCCCTTGAGCGTTTCGCAGCCGGTCAAGAGCTACGCCCGTGCCGCCCGTGCA
ATCGAAAACATCGATTTCCAGCGCGGCGACCAAGGTGAGGGCAATGTGGTGATCACGCTGTCCGATCCGAGCGTTGCCCC
GGACATTCAGGAGCAGGGTGGCCGGATCCGCCTGGACTTTTCCAAGACCCAGTTGCCCGAGTCCCTGCGCGTGCGACTGG
ACGTGAAGGACTTTGCCACGCCGGTGCAGTTCGTCAGCGCATCGGGGAGCGGTGCCAACGCCAGCATCAGCATCGAGCCG
ACGGGGTTCTATGACTACCTGGCCTACCAGACCGACAACAAGCTGACCGTCAGCATCAAGCCGCTGACCCAGGACGATGT
GGAGAAACGCAAGGCCGAGCGTTTTGCCTACTCCGGCGAAAAGCTGTCGCTGAACTTCCAGGACATCGACGTGCGTTCGG
TCCTGCAGCTGATCGCTGATTTCACCGATCTGAACCTGGTGGCTTCGGACACCGTGCAGGGCAACATCACGCTACGCCTG
CAGAATGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGGTCGGTAACGTGCT
GCTGGTCGCGCCGGCTGACGAGATCGCGGCCCGCGAACGCCAGGAGCTGGAGTCGCAGAAGCAGATTGCCGAACTGGCGC
CCCTGCGCCGCGAGCTGATCCAGGTGAACTACGCCAAGGCGGCGGACATCGCCAAGTTGTTCCAGTCGGTTACCAGCGGC
GATCCGAGCGCTGTCGGCACCGATGAGCGTGGTTCCATCACCGTGGATGACCGCACCAACAGCATTATCGCCTATCAGAC
TCAGGACAAGCTGGACGAGCTGCGCCGCATTGTCACCCAGCTGGATATTCCGGTGCGCCAGGTGATGATCGAAGCTCGCA
TCGTCGAAGCCAACGTCGACTACGACAAGGCGCTGGGCGTGAACTGGCGCGGTGCCAGCATTGGTGACAACAATTTCGTC
GTCGGTGGTGGCGGCGCTGGCCGCCCGGCCGCTGGCCAGGCCGGTAACGTCAACCTGGCCAATGCGCCGTTCGTCGATCT
CGGTGCCTCCGGCGCTACCTCGAGCATCGGTATCGGCTTCATTACCGACAACACGATCCTCGATCTGGAACTCTCGGCGA
TGGAGAAGACCGGTAATGGTGAAATCGTGTCCCAGCCCAAGGTGGTCACTTCGGACAAGGAAACCGCGAAGATCCTGAAA
GGCGCCGAGATCCCGTACCAGGAAGCCAGTTCCAGCGGTGCCACCAGCACGTCCTTCAAGGAGGCTGCCCTGTCCCTGGA
GGTGACGCCGCAAATCACGCCGGACAACCGCATCATCATGGAAGTCAAGGTCACCAAGGATGCGCCCGACTTCGCCAATG
CCCTGAACGGCGTACCGCCGATCAATAAAAACGAGGTCAACGCCAAGGTGCTGGTCAACGATGGTGAAACCATCGTCATA
GGTGGGGTATTCTCCAACACCCAAACCAAGGCGGTGGACAAGGTGCCCTTCCTCGGCGACCTGCCTTTCCTCGGCCGCAT
GTTCCGGCGCGACGTGGTGCAGGACAAGAAAGCCGAGCTCCTGGTATTCCTCACTCCCCGCATCATGAACAACCAGGCCA
TTGCGGTGAGTCGCTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-119)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-447)

Secretin and TonB N terminus short domain (STN)

(305-351)

Bacterial type II and III secretion system protein (Secretin)

(533-689)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

77.311

100

0.791
  comQ Acinetobacter baylyi ADP1

43.723

99.284

0.434
  pilQ Acinetobacter baumannii strain A118

41.408

100

0.421
  pilQ Acinetobacter baumannii D1279779

41.408

100

0.421
  pilQ Legionella pneumophila strain Lp02

38.496

97.135

0.374
  pilQ Legionella pneumophila strain ERS1305867

38.348

97.135

0.372