Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   PJW05_RS24625 Genome accession   NZ_CP116304
Coordinates   5340686..5341750 (-) Length   354 a.a.
NCBI ID   WP_271409546.1    Uniprot ID   -
Organism   Pseudomonas sp. Q1-7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5335686..5346750
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJW05_RS24600 aroK 5336247..5336765 (-) 519 WP_271409541.1 shikimate kinase AroK -
  PJW05_RS24605 pilQ 5336771..5338867 (-) 2097 WP_271409542.1 type IV pilus secretin PilQ Machinery gene
  PJW05_RS24610 pilP 5338922..5339455 (-) 534 WP_271409543.1 type 4a pilus biogenesis lipoprotein PilP -
  PJW05_RS24615 pilO 5339452..5340075 (-) 624 WP_271409544.1 type 4a pilus biogenesis protein PilO -
  PJW05_RS24620 pilN 5340072..5340686 (-) 615 WP_271409545.1 type 4a pilus biogenesis protein PilN -
  PJW05_RS24625 pilM 5340686..5341750 (-) 1065 WP_271409546.1 pilus assembly protein PilM Machinery gene
  PJW05_RS24630 - 5341935..5344370 (+) 2436 WP_271409547.1 penicillin-binding protein 1A -
  PJW05_RS24635 - 5344422..5345690 (-) 1269 WP_271409548.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37819.08 Da        Isoelectric Point: 4.4486

>NTDB_id=776520 PJW05_RS24625 WP_271409546.1 5340686..5341750(-) (pilM) [Pseudomonas sp. Q1-7]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGSRFKVESYAVEPLPPNAVVEKNIAELEGVGQALSRVVTKARTGVKAAAV
AVAGSAVITKTIEMEAGLSDDDLENQLKIEADQYIPYPLEEVAIDFEVQGASARNPERVDVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYSLLSAQLGDAHDELTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKDAVVQQVSRSLQFFFAAGQFNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLAMRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=776520 PJW05_RS24625 WP_271409546.1 5340686..5341750(-) (pilM) [Pseudomonas sp. Q1-7]
GTGCTAGGGCTCTTCAATAAGAAAGCGAATTCGCTGTTGGGGATCGACATCAGCTCGACCTCAGTCAAGCTCCTTGAATT
GAGTCGCTCGGGAAGCCGCTTCAAAGTGGAGTCCTATGCCGTCGAGCCACTCCCGCCGAACGCGGTTGTCGAAAAGAACA
TCGCGGAGCTGGAGGGTGTCGGGCAGGCGCTTTCCCGTGTGGTCACCAAGGCGCGAACCGGTGTCAAGGCTGCCGCGGTA
GCCGTGGCAGGGTCGGCCGTGATCACCAAAACCATCGAGATGGAAGCTGGGCTCTCCGATGACGATCTGGAGAACCAGCT
GAAGATCGAGGCTGACCAGTACATTCCTTATCCATTGGAAGAGGTCGCCATCGACTTCGAGGTGCAAGGCGCCTCCGCGC
GCAATCCTGAACGGGTCGACGTGCTGCTGGCGGCCTGCCGCAAGGAAAACGTCGAAGTGCGCGAGGCCGCGCTGGCTCTG
GCTGGGCTCACCGCCAAGGTGGTCGACGTCGAGGCCTATGCGCTGGAGCGTGCGTACTCTTTGCTCTCCGCCCAGCTCGG
CGATGCCCATGACGAGCTGACCGTGGCCGTCGTCGACATCGGTGCCACGATGACCACCCTCAGCGTGCTGCACAACGGTC
GCACCATCTATACCCGCGAGCAGTTGTTCGGCGGTCGCCAGCTGACCGAGGAGATTCAGCGCCGCTATGGGCTTTCGGTC
GAGGAGGCGGGTCTGGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAGGTCCTTCAGCCGTTCAAGGATGC
GGTGGTCCAGCAGGTCTCCCGCTCGCTGCAGTTCTTCTTCGCGGCCGGCCAGTTCAATGATGTGGACTACATCCTCCTGG
CGGGTGGCACGGCTTCGATTCCTGATCTGGATCGCCTCATCCAGCAGAAGATCGGCACTCCGACCCTGGTCGCCAATCCC
TTCGCCGACATGGCGCTGAGCAGCAAGGTCAATGCAGGTGCGCTCGCCAGTGATGCGCCCGCGCTGATGATCGCTTGCGG
CTTGGCGATGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.78

100

0.568
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  comM Acinetobacter baylyi ADP1

53.107

100

0.531
  pilM Legionella pneumophila strain ERS1305867

46.61

100

0.466