Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PJW05_RS05525 Genome accession   NZ_CP116304
Coordinates   1196066..1197772 (-) Length   568 a.a.
NCBI ID   WP_271410729.1    Uniprot ID   -
Organism   Pseudomonas sp. Q1-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1191066..1202772
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PJW05_RS05490 - 1191237..1191659 (+) 423 WP_271410723.1 GNAT family N-acetyltransferase -
  PJW05_RS05495 - 1191664..1192131 (+) 468 WP_271410724.1 FAD/FMN-containing dehydrogenase -
  PJW05_RS05500 - 1192128..1192817 (+) 690 WP_271410725.1 energy-coupling factor ABC transporter permease -
  PJW05_RS05505 yacG 1193166..1193366 (-) 201 WP_151131890.1 DNA gyrase inhibitor YacG -
  PJW05_RS05510 coaE 1193363..1193974 (-) 612 WP_271410726.1 dephospho-CoA kinase -
  PJW05_RS05515 pilD 1193971..1194843 (-) 873 WP_271410727.1 A24 family peptidase Machinery gene
  PJW05_RS05520 pilC 1194846..1196063 (-) 1218 WP_271410728.1 type II secretion system F family protein Machinery gene
  PJW05_RS05525 pilB 1196066..1197772 (-) 1707 WP_271410729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PJW05_RS05530 - 1198059..1198535 (+) 477 WP_271410730.1 pilin -
  PJW05_RS05535 - 1198606..1200270 (+) 1665 WP_271410731.1 O-antigen ligase family protein -
  PJW05_RS05545 - 1200447..1200674 (-) 228 WP_271410732.1 hypothetical protein -
  PJW05_RS05550 - 1201124..1201414 (+) 291 WP_271410733.1 type II toxin-antitoxin system RelE/ParE family toxin -
  PJW05_RS05555 - 1201424..1201735 (+) 312 WP_271410734.1 addiction module antidote protein -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62616.52 Da        Isoelectric Point: 5.3300

>NTDB_id=776505 PJW05_RS05525 WP_271410729.1 1196066..1197772(-) (pilB) [Pseudomonas sp. Q1-7]
MTETASTLSGLAKQLVLTNLLDERSALQAHTQAQRNKIPLITYLVQNKLLKSRDLAEMAADQFGVAFLDLAGIDKESQPR
DLVSEKLVRQHRALPLWRRGNKLFVAISDPTNHQAVTDIQFSTGLNTEAILVEDDKLGDAIEKFFDSAGSGMEDLGDVDL
DGIDVEAVDDDKNDGPGGADADDAPVVRFVNKMLLDAIRGGSSDLHFEPYERAYRVRFRTDGMLHEVAKPPIQLASRISA
RLKVMAALDISERRKPQDGRIKMKISKTKSIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYLTALKQP
QGMILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPKQGMDFAQALRAFLRQDPDVIMVGEIR
DLETAEIAIKAAQTGHMVLSTLHTNSAAETLTRLRNMGVPAFNIATSVNLIIAQRLARKLCGHCKKEHEVPRETLLKEGF
PEDRIGSFTLYAPVGCENCNQGYKGRVGIYEVVKITPAMQQLIMEEGNSIQIAQQARRDGFNDLRTSGLVKAMNGITSLE
EVNRVTKD

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=776505 PJW05_RS05525 WP_271410729.1 1196066..1197772(-) (pilB) [Pseudomonas sp. Q1-7]
ATGACCGAGACCGCATCCACTCTTTCTGGCCTGGCCAAACAACTGGTACTGACCAATCTCCTCGATGAGCGGAGCGCCCT
GCAGGCCCATACCCAGGCGCAGCGTAACAAGATTCCGCTGATCACCTACCTGGTGCAGAACAAGCTGCTGAAAAGCCGCG
ACCTGGCGGAGATGGCCGCGGACCAGTTCGGCGTCGCCTTTCTCGACCTCGCCGGCATAGACAAAGAATCCCAACCCAGG
GACCTGGTGAGCGAGAAACTGGTGCGCCAGCACCGCGCCCTGCCCCTGTGGCGCCGCGGCAACAAGCTGTTCGTCGCCAT
CTCCGACCCCACCAATCATCAGGCGGTGACGGATATCCAGTTCAGCACCGGCCTGAATACTGAAGCCATCCTGGTCGAAG
ACGACAAACTGGGCGACGCCATCGAAAAGTTCTTCGACTCCGCCGGCAGCGGCATGGAGGACCTGGGAGACGTCGACCTG
GACGGCATCGACGTCGAGGCGGTGGATGACGACAAGAACGATGGCCCCGGTGGCGCGGATGCCGATGACGCGCCGGTGGT
GCGTTTCGTCAACAAGATGCTGCTGGATGCCATCCGCGGCGGCTCGTCGGACCTGCACTTCGAGCCTTATGAGCGCGCCT
ACCGGGTCCGCTTCCGCACCGATGGCATGCTGCATGAGGTGGCCAAGCCGCCGATCCAATTGGCCAGCCGTATCTCGGCC
CGCCTCAAGGTGATGGCCGCCCTGGATATTTCCGAACGCCGCAAACCCCAGGACGGCCGGATCAAGATGAAGATTTCCAA
GACGAAATCCATCGACTTCCGCGTCAACACCCTGCCCACCCTCTGGGGCGAGAAGATCGTGATGCGGATTCTCGACCCCA
CCAGCGCGCAGATGGGTATCGATGCCCTGGGTTACGAGGAAGAACAGAAGGAGCTGTACCTCACGGCCCTGAAGCAGCCC
CAGGGGATGATCCTGGTCACCGGCCCCACCGGTTCGGGCAAGACCGTCTCCCTTTATACGGGCCTGAACATCCTCAACAC
GGTGGATATCAACATCTCCACGGCGGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTGAACCCCA
AGCAGGGCATGGATTTCGCCCAGGCCCTGCGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGC
GACCTGGAAACCGCCGAGATCGCCATCAAGGCTGCCCAGACCGGCCACATGGTGCTATCCACCCTGCACACCAATAGCGC
CGCCGAGACCTTGACCCGCCTGCGCAACATGGGCGTACCGGCGTTCAATATCGCCACCTCGGTCAACCTGATCATCGCCC
AGCGCCTGGCACGCAAATTGTGTGGCCATTGCAAGAAGGAGCATGAGGTGCCGCGGGAGACCCTGCTCAAGGAAGGCTTT
CCCGAAGACCGGATCGGCAGCTTCACGCTCTATGCCCCGGTCGGCTGCGAGAACTGCAACCAGGGCTACAAGGGCCGAGT
GGGTATTTATGAAGTGGTTAAAATCACGCCGGCCATGCAGCAGCTTATTATGGAAGAAGGCAACTCCATCCAGATCGCCC
AGCAGGCGCGCCGCGATGGCTTCAATGACCTGCGAACCTCGGGTCTGGTGAAAGCCATGAACGGCATCACCAGCCTGGAG
GAAGTCAACCGCGTGACTAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(44-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.848

99.824

0.577
  pilB Acinetobacter baumannii D1279779

57.57

100

0.576
  pilB Legionella pneumophila strain ERS1305867

57.143

99.824

0.57
  pilB Vibrio parahaemolyticus RIMD 2210633

50.265

99.824

0.502
  pilF Neisseria gonorrhoeae MS11

49.378

99.12

0.489
  pilB Vibrio campbellii strain DS40M4

48.582

99.296

0.482
  pilB Vibrio cholerae strain A1552

52.29

92.254

0.482
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.698

93.134

0.37
  pilF Thermus thermophilus HB27

39.171

93.486

0.366