Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PE066_RS15385 Genome accession   NZ_CP116009
Coordinates   3226334..3228073 (-) Length   579 a.a.
NCBI ID   WP_271233405.1    Uniprot ID   -
Organism   Ramlibacter tataouinensis strain DMF-7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3221334..3233073
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PE066_RS15355 (PE066_15355) - 3222146..3222619 (+) 474 WP_271233399.1 NUDIX domain-containing protein -
  PE066_RS15360 (PE066_15360) - 3222623..3222838 (-) 216 WP_271233400.1 DNA gyrase inhibitor YacG -
  PE066_RS15365 (PE066_15365) zapD 3222841..3223596 (-) 756 WP_271233401.1 cell division protein ZapD -
  PE066_RS15370 (PE066_15370) coaE 3223634..3224242 (-) 609 WP_271233402.1 dephospho-CoA kinase -
  PE066_RS15375 (PE066_15375) pilD 3224245..3225084 (-) 840 WP_271233403.1 A24 family peptidase Machinery gene
  PE066_RS15380 (PE066_15380) pilC 3225084..3226301 (-) 1218 WP_271233404.1 type II secretion system F family protein Machinery gene
  PE066_RS15385 (PE066_15385) pilB 3226334..3228073 (-) 1740 WP_271233405.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PE066_RS15395 (PE066_15395) - 3228346..3229275 (-) 930 WP_271236593.1 polyprenyl synthetase family protein -
  PE066_RS15400 (PE066_15400) rplU 3229469..3229780 (+) 312 WP_271233406.1 50S ribosomal protein L21 -
  PE066_RS15405 (PE066_15405) rpmA 3229800..3230057 (+) 258 WP_271233407.1 50S ribosomal protein L27 -
  PE066_RS15410 (PE066_15410) cgtA 3230139..3231221 (+) 1083 WP_271233408.1 Obg family GTPase CgtA -
  PE066_RS15415 (PE066_15415) proB 3231218..3232372 (+) 1155 WP_271233409.1 glutamate 5-kinase -
  PE066_RS15420 (PE066_15420) - 3232383..3232898 (-) 516 WP_271233410.1 CNP1-like family protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 63012.04 Da        Isoelectric Point: 5.2331

>NTDB_id=774922 PE066_RS15385 WP_271233405.1 3226334..3228073(-) (pilB) [Ramlibacter tataouinensis strain DMF-7]
MAADPALSDAPSLALPGLARALVAAGKLGQKSAEELYRKAQSNRSSFIAELTGSGAVSPSDLAHTMSLSYGAPLLDLDAV
DVQWLPKSLLDPKLCQAFRVVVLSKRNNRLTVATADPSDQQAADKIKFATQMGVDWVIAEYDKLTKMVEAHATSATQAME
SIVGDDFEFDESTMDDSVVDDEDKGSAGSEVDDAPVVKFLHKMLLDAFSMRASDLHFEPYEHTYRVRFRIDGELREIASP
PIAIKDKLASRVKVISRMDISEKRVPQDGRMKLKVGPDRVIDFRVSTLPTLFGEKIVIRILDPSSAKLGIEALGYEAEEK
ERLLNAIGRPYGMVLVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPSEINLPGVNQVNVNEKAGLTFAAALKSFLRQD
PDVIMVGEIRDLETADIAIKAAQTGHMVMSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLARRLCANCKQPADI
PHETLIEAGFTEEEVDGTWTPYRPVGCGACNNGYKGRVGIYQVMPVSEEIQRIILRDGSAMEIAEQSRREGVKSLRQSGL
HKVRLGVTSLEEVLGCTNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=774922 PE066_RS15385 WP_271233405.1 3226334..3228073(-) (pilB) [Ramlibacter tataouinensis strain DMF-7]
ATGGCCGCCGACCCTGCACTCTCCGACGCTCCCTCGCTGGCACTGCCCGGGCTGGCGCGGGCCCTGGTGGCGGCCGGCAA
GCTCGGCCAGAAATCGGCCGAGGAGCTGTATCGCAAGGCGCAGTCCAACCGCAGCAGTTTCATTGCCGAGCTGACCGGCT
CCGGCGCGGTGTCGCCGTCCGACCTGGCGCACACCATGTCGCTCTCCTACGGCGCGCCGCTGCTCGACCTGGACGCAGTC
GACGTGCAGTGGCTGCCGAAGAGCCTGCTGGACCCCAAGCTGTGCCAGGCGTTCCGGGTGGTGGTGCTGTCCAAGCGCAA
CAACCGGCTGACCGTCGCCACCGCCGACCCCTCCGACCAGCAGGCCGCCGACAAGATCAAGTTCGCCACCCAGATGGGCG
TGGACTGGGTCATCGCCGAGTACGACAAGCTGACCAAGATGGTCGAGGCCCACGCCACCAGCGCCACCCAGGCGATGGAG
AGCATCGTCGGCGACGATTTCGAGTTCGACGAGTCGACCATGGACGACTCGGTGGTCGACGACGAGGACAAGGGCTCGGC
CGGCTCGGAGGTCGACGACGCGCCGGTCGTCAAGTTCCTGCACAAGATGCTGCTCGACGCGTTCAGCATGCGCGCGTCCG
ACCTGCACTTCGAGCCCTACGAGCACACCTACCGGGTCCGCTTCCGCATCGACGGCGAACTGCGCGAGATCGCCTCGCCG
CCCATCGCCATCAAGGACAAGCTGGCCTCGCGGGTCAAGGTCATCTCGCGGATGGACATCTCCGAGAAGCGGGTGCCGCA
GGACGGCCGCATGAAGCTCAAGGTCGGACCCGACCGGGTGATCGACTTCCGCGTCAGCACGCTGCCCACGCTGTTCGGCG
AGAAGATCGTGATCCGGATCCTGGATCCGAGCAGCGCCAAGCTGGGCATTGAGGCTCTCGGCTACGAAGCCGAGGAGAAG
GAGCGCCTGCTCAACGCCATCGGCCGGCCCTACGGCATGGTGCTGGTCACCGGCCCCACCGGTTCGGGCAAGACCGTGTC
CCTCTACACCTGCCTGAACCTGCTGAACAAGCCGGGGGTGAACATCTCGACGGCCGAAGACCCGTCCGAAATCAACCTGC
CCGGCGTCAACCAGGTCAACGTCAACGAGAAGGCCGGGCTGACCTTCGCCGCAGCGCTCAAGTCGTTCCTGCGCCAGGAT
CCCGACGTCATCATGGTCGGCGAGATCCGCGACCTGGAGACCGCCGACATCGCGATCAAGGCCGCGCAGACCGGCCACAT
GGTGATGTCCACGCTGCACACCAACGACGCGCCCACCACGCTCACGCGCATGCGCAACATGGGCATCGCGCCGTTCAACA
TCGCCTCCAGCGTGATCCTGATCACCGCGCAGCGGCTGGCGCGGCGCCTGTGCGCCAACTGCAAGCAGCCGGCCGACATC
CCGCACGAGACGCTGATCGAAGCGGGCTTCACCGAGGAGGAAGTCGATGGCACCTGGACCCCGTACCGCCCGGTCGGCTG
CGGCGCCTGCAACAACGGCTACAAGGGCCGGGTCGGCATCTACCAGGTGATGCCCGTCAGCGAGGAGATTCAGCGCATCA
TCCTGCGCGACGGCAGCGCCATGGAGATCGCCGAGCAGTCCCGCCGCGAAGGCGTCAAGAGCCTGCGGCAATCGGGCCTG
CACAAGGTCCGGCTGGGCGTCACTTCGCTCGAGGAAGTGCTCGGCTGCACCAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(202-469)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(52-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.13

99.309

0.547
  pilB Acinetobacter baylyi ADP1

53.541

100

0.535
  pilF Neisseria gonorrhoeae MS11

51.943

97.755

0.508
  pilB Legionella pneumophila strain ERS1305867

49.378

97.237

0.48
  pilB Vibrio campbellii strain DS40M4

44.938

97.237

0.437
  pilB Vibrio parahaemolyticus RIMD 2210633

44.964

96.028

0.432
  pilB Vibrio cholerae strain A1552

46.947

90.501

0.425
  pilF Thermus thermophilus HB27

39.753

97.755

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.149

92.919

0.373