Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PG909_RS02720 Genome accession   NZ_CP115946
Coordinates   566269..566790 (+) Length   173 a.a.
NCBI ID   WP_003278951.1    Uniprot ID   -
Organism   Ralstonia solanacearum MolK2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 561269..571790
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG909_RS02700 sucC 561898..563064 (+) 1167 WP_003261636.1 ADP-forming succinate--CoA ligase subunit beta -
  PG909_RS02705 sucD 563143..564024 (+) 882 WP_003278945.1 succinate--CoA ligase subunit alpha -
  PG909_RS02710 - 564151..565274 (+) 1124 WP_155402205.1 IS3 family transposase -
  PG909_RS02715 - 565387..566085 (+) 699 WP_003278949.1 TerC family protein -
  PG909_RS02720 pilA 566269..566790 (+) 522 WP_003278951.1 pilin Machinery gene
  PG909_RS02725 pilA 567058..567564 (+) 507 WP_003278953.1 pilin Machinery gene
  PG909_RS02730 - 567639..569372 (+) 1734 WP_042550425.1 PglL family O-oligosaccharyltransferase -
  PG909_RS02735 moaC 569449..569925 (-) 477 WP_013206902.1 cyclic pyranopterin monophosphate synthase MoaC -
  PG909_RS02740 - 570041..571747 (+) 1707 WP_042550426.1 tetratricopeptide repeat protein -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17606.35 Da        Isoelectric Point: 10.0319

>NTDB_id=774562 PG909_RS02720 WP_003278951.1 566269..566790(+) (pilA) [Ralstonia solanacearum MolK2]
MASKHSRNRRRGQRGFTLIELMIVVAIIGILAAIAIPAYQDYTIRARVVEGLSLAAQAKALVVENAANAQSSLALGSASL
PASKNVSALNIDASTGEIAVVYATAVTAGGANTLVLTPYSGGSSAFANLHAGSAPPAMVLWVCAAAGKAMPVTTVTQNTT
ATLPPKYAPAECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=774562 PG909_RS02720 WP_003278951.1 566269..566790(+) (pilA) [Ralstonia solanacearum MolK2]
ATGGCATCGAAGCACAGCAGGAACAGGCGGCGCGGTCAGCGCGGCTTTACGTTGATCGAGTTGATGATCGTGGTGGCGAT
CATCGGGATACTGGCGGCCATTGCGATTCCGGCGTACCAGGACTACACGATCCGGGCGCGGGTGGTTGAGGGGCTGTCGC
TGGCCGCGCAGGCCAAGGCACTGGTGGTTGAGAATGCTGCCAACGCCCAGAGCAGCTTGGCGCTCGGCTCCGCATCATTG
CCGGCGAGCAAGAATGTCAGTGCGCTGAATATCGATGCCTCTACCGGCGAGATTGCGGTGGTCTATGCGACAGCCGTCAC
GGCAGGTGGGGCGAACACGCTGGTCTTGACTCCATACTCGGGAGGCTCGTCGGCATTTGCTAACTTGCACGCTGGCTCTG
CGCCGCCGGCAATGGTGTTGTGGGTGTGCGCTGCCGCCGGAAAGGCTATGCCGGTAACCACCGTCACCCAGAACACGACG
GCTACGTTGCCGCCGAAATATGCGCCCGCGGAATGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(45-173)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

55.682

100

0.566
  pilA2 Legionella pneumophila str. Paris

46.667

95.376

0.445
  pilA2 Legionella pneumophila strain ERS1305867

46.061

95.376

0.439
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.566

100

0.41
  comP Acinetobacter baylyi ADP1

42.857

93.064

0.399
  pilE Neisseria gonorrhoeae MS11

36.628

99.422

0.364