Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   PG909_RS02725 Genome accession   NZ_CP115946
Coordinates   567058..567564 (+) Length   168 a.a.
NCBI ID   WP_003278953.1    Uniprot ID   -
Organism   Ralstonia solanacearum MolK2     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 562058..572564
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PG909_RS02705 sucD 563143..564024 (+) 882 WP_003278945.1 succinate--CoA ligase subunit alpha -
  PG909_RS02710 - 564151..565274 (+) 1124 WP_155402205.1 IS3 family transposase -
  PG909_RS02715 - 565387..566085 (+) 699 WP_003278949.1 TerC family protein -
  PG909_RS02720 pilA 566269..566790 (+) 522 WP_003278951.1 pilin Machinery gene
  PG909_RS02725 pilA 567058..567564 (+) 507 WP_003278953.1 pilin Machinery gene
  PG909_RS02730 - 567639..569372 (+) 1734 WP_042550425.1 PglL family O-oligosaccharyltransferase -
  PG909_RS02735 moaC 569449..569925 (-) 477 WP_013206902.1 cyclic pyranopterin monophosphate synthase MoaC -
  PG909_RS02740 - 570041..571747 (+) 1707 WP_042550426.1 tetratricopeptide repeat protein -
  PG909_RS02745 - 571766..572353 (-) 588 WP_003278960.1 DUF2946 family protein -

Sequence

Protein


Download         Length: 168 a.a.        Molecular weight: 16956.60 Da        Isoelectric Point: 9.9749

>NTDB_id=774563 PG909_RS02725 WP_003278953.1 567058..567564(+) (pilA) [Ralstonia solanacearum MolK2]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAANAQSDLSVGSSVFT
ATKNVANLTIAGTGGVPGQITVTYTTAAGGGTLALVPTAAGSALPISSAPSGPIMWTCYAQNKAAAASSVSPSGTMTLAA
KYAPAECR

Nucleotide


Download         Length: 507 bp        

>NTDB_id=774563 PG909_RS02725 WP_003278953.1 567058..567564(+) (pilA) [Ralstonia solanacearum MolK2]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGCTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCTATCGCCATTCCGGCTTATCAGGACTACACGGTTCGTGCGCGCGTGACGGAAGGTCTGTCGCTGG
CTGCGCAAGCCAAGGCACTGGTGTCGGAAAACGCTGCCAATGCACAGTCTGACCTGTCGGTGGGGTCGTCGGTGTTCACG
GCCACCAAGAACGTTGCCAACTTGACGATTGCGGGTACGGGCGGTGTTCCGGGTCAGATCACCGTCACCTATACGACGGC
AGCTGGTGGCGGCACGCTGGCGCTGGTTCCGACTGCGGCTGGTTCTGCACTGCCGATTAGCTCGGCACCGTCGGGCCCGA
TCATGTGGACCTGCTACGCTCAAAACAAGGCCGCTGCTGCCAGCAGCGTTTCGCCGAGCGGTACGATGACGCTGGCGGCC
AAGTATGCCCCGGCAGAGTGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-168)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

95.833

100

0.958
  pilA2 Legionella pneumophila str. Paris

50.932

95.833

0.488
  pilA2 Legionella pneumophila strain ERS1305867

50.311

95.833

0.482
  comP Acinetobacter baylyi ADP1

45.614

100

0.464
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

37.629

100

0.435
  comF Acinetobacter baylyi ADP1

37.805

97.619

0.369