Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O6P39_RS04830 Genome accession   NZ_CP115669
Coordinates   1027695..1029398 (-) Length   567 a.a.
NCBI ID   WP_275610277.1    Uniprot ID   -
Organism   Pseudomonas sp. PSE14     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1022695..1034398
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O6P39_RS04790 (O6P39_04790) - 1022786..1023202 (+) 417 WP_275610270.1 GNAT family N-acetyltransferase -
  O6P39_RS04795 (O6P39_04795) - 1023205..1023672 (+) 468 WP_275610271.1 FAD/FMN-containing dehydrogenase -
  O6P39_RS04800 (O6P39_04800) - 1023669..1024358 (+) 690 WP_275610272.1 energy-coupling factor ABC transporter permease -
  O6P39_RS04805 (O6P39_04805) - 1024466..1024681 (+) 216 WP_275610273.1 hypothetical protein -
  O6P39_RS04810 (O6P39_04810) yacG 1024722..1024925 (-) 204 WP_015475735.1 DNA gyrase inhibitor YacG -
  O6P39_RS04815 (O6P39_04815) coaE 1024922..1025530 (-) 609 WP_275610274.1 dephospho-CoA kinase -
  O6P39_RS04820 (O6P39_04820) pilD 1025596..1026468 (-) 873 WP_275610275.1 A24 family peptidase Machinery gene
  O6P39_RS04825 (O6P39_04825) pilC 1026474..1027691 (-) 1218 WP_275610276.1 type II secretion system F family protein Machinery gene
  O6P39_RS04830 (O6P39_04830) pilB 1027695..1029398 (-) 1704 WP_275610277.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O6P39_RS04835 (O6P39_04835) - 1029625..1030059 (+) 435 WP_275610278.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  O6P39_RS04845 (O6P39_04845) galE 1030274..1031296 (-) 1023 WP_275610279.1 UDP-glucose 4-epimerase GalE -
  O6P39_RS04850 (O6P39_04850) nadC 1031597..1032445 (-) 849 WP_275610280.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62743.84 Da        Isoelectric Point: 5.6264

>NTDB_id=773412 O6P39_RS04830 WP_275610277.1 1027695..1029398(-) (pilB) [Pseudomonas sp. PSE14]
MNDQVVLSGLARQLVLSQLLDENTAREAQSQAQRNKLSLVTYLVQNKLVKSRPMMELAADQFGVAFCDLSSLDRDSFPKE
LVSEKLVRQHRVLPLWRRGNKLFVGLSDPTNHQAITDIQFSTGLTTEAILVEDDRLGDAIEKLFESVTGGLDDLGDVDLE
GLDIESGNAERKEENIGGDADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKVYRVRFRTDGMLQEVAKPPVQLVSRISAR
LKVMAGLDISERRKPQDGRIKMRVSKTKSIDFRVNTLPTLWGEKIVMRILDSASAQMGIDALGYEEGQKQLYLDALKKPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDINISTAEDPVEINLEGINQVNVNPKQGLDFAQALRAFLRQDPDVIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVASFNLATSTNLIIAQRLARKLCPTCKQEHDVPRETLLHEGFP
ENRVGSFKLYSPVGCENCKNGYKGRVGIYEVVKITPALQRIIMEEGNSIQIAEQARKEGFNDLRTSGLLKAMQGITSLEE
INRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=773412 O6P39_RS04830 WP_275610277.1 1027695..1029398(-) (pilB) [Pseudomonas sp. PSE14]
ATGAACGATCAAGTCGTCCTGTCCGGCCTTGCCCGGCAACTGGTGTTGTCTCAACTGCTCGACGAGAATACCGCGCGAGA
GGCCCAATCGCAGGCACAGCGCAACAAGCTGTCGCTGGTGACCTACCTGGTGCAGAACAAGCTGGTGAAAAGCCGCCCGA
TGATGGAGTTGGCCGCCGACCAGTTCGGCGTCGCCTTTTGTGATCTGAGCAGTCTGGATAGAGATAGCTTTCCCAAAGAA
TTGGTCAGCGAGAAGCTCGTTCGCCAACACCGCGTCCTCCCTCTGTGGCGCAGAGGAAACAAGCTCTTCGTCGGCCTGTC
GGACCCGACCAACCACCAAGCGATCACCGACATCCAGTTCAGCACAGGCCTAACCACCGAAGCCATCCTGGTGGAGGATG
ATCGGCTCGGCGACGCCATCGAGAAACTCTTCGAGAGCGTTACCGGTGGGCTGGACGACCTAGGCGATGTCGACCTGGAA
GGCCTGGATATCGAAAGCGGTAATGCCGAGCGCAAGGAAGAAAACATCGGCGGCGATGCCGACGACGCGCCCGTGGTGCG
CTTCGTCAACAAGATGCTGCTCGACGCCATCAAGGGCGGCTCTTCCGACTTGCACTTCGAGCCCTATGAGAAGGTCTACC
GGGTGCGTTTCCGTACCGATGGCATGCTCCAGGAAGTCGCCAAACCGCCGGTGCAACTGGTCAGCCGCATCTCCGCCCGA
CTCAAAGTGATGGCCGGTCTGGATATCTCCGAGCGCCGCAAGCCGCAGGACGGCCGCATCAAGATGCGCGTATCCAAGAC
CAAGTCCATCGACTTCCGCGTCAACACGCTGCCCACCCTTTGGGGCGAAAAGATCGTGATGCGTATTCTCGACTCGGCCA
GCGCGCAGATGGGTATCGATGCCCTGGGCTACGAGGAGGGACAGAAACAGCTGTATCTCGATGCGCTGAAAAAGCCGCAG
GGCATGATCCTGGTGACCGGCCCCACCGGGTCGGGCAAGACGGTATCCCTCTATACCGGCCTGAACATCCTCAATACGCC
AGATATCAATATCTCGACTGCGGAAGATCCAGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGAAAC
AGGGCCTGGACTTCGCCCAAGCCCTGCGCGCCTTCCTGCGTCAGGACCCCGACGTGATCATGGTAGGTGAGATCCGCGAC
CTGGAAACGGCGGAAATCGCCATCAAGGCCGCGCAAACCGGGCACATGGTGATGTCCACCCTGCACACCAACAGCGCCGC
GGAAACCCTGACGCGCCTACTGAACATGGGGGTTGCCTCCTTCAACCTCGCCACATCGACTAACCTGATCATTGCCCAGC
GTCTGGCACGCAAGCTCTGCCCAACTTGCAAGCAGGAACACGACGTACCGCGCGAAACCCTGCTTCACGAAGGCTTTCCG
GAAAACCGCGTAGGTTCGTTCAAGCTCTATTCCCCAGTCGGCTGCGAAAACTGCAAGAACGGCTACAAGGGGCGTGTGGG
TATTTATGAAGTGGTTAAAATCACGCCGGCACTACAGCGGATTATCATGGAAGAAGGCAATTCCATTCAGATCGCCGAAC
AAGCCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCGGGCCTGCTGAAAGCCATGCAGGGCATTACCAGCCTCGAGGAA
ATCAACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.699

99.647

0.575
  pilB Acinetobacter baylyi ADP1

57.801

99.471

0.575
  pilB Legionella pneumophila strain ERS1305867

54.674

100

0.547
  pilF Neisseria gonorrhoeae MS11

49.912

100

0.499
  pilB Vibrio parahaemolyticus RIMD 2210633

52.126

95.414

0.497
  pilB Vibrio campbellii strain DS40M4

52.222

95.238

0.497
  pilB Vibrio cholerae strain A1552

49.911

99.295

0.496
  pilF Thermus thermophilus HB27

38.596

100

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.983

93.651

0.365