Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   H7K70_RS26860 Genome accession   NZ_AP022213
Coordinates   5776168..5778294 (-) Length   708 a.a.
NCBI ID   WP_182851205.1    Uniprot ID   A0A6S5RWP9
Organism   Metapseudomonas otitidis strain WP8-S17-CRE-03     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5771168..5783294
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  H7K70_RS26845 (WP8S17C03_52460) - 5772810..5774477 (-) 1668 WP_182851202.1 SPOR domain-containing protein -
  H7K70_RS26850 (WP8S17C03_52470) aroB 5774488..5775591 (-) 1104 WP_182851203.1 3-dehydroquinate synthase -
  H7K70_RS26855 (WP8S17C03_52480) aroK 5775644..5776162 (-) 519 WP_182851204.1 shikimate kinase AroK -
  H7K70_RS26860 (WP8S17C03_52490) pilQ 5776168..5778294 (-) 2127 WP_182851205.1 type IV pilus secretin PilQ Machinery gene
  H7K70_RS26865 (WP8S17C03_52500) pilP 5778349..5778879 (-) 531 WP_182852983.1 type 4a pilus biogenesis lipoprotein PilP -
  H7K70_RS26870 (WP8S17C03_52510) pilO 5778879..5779502 (-) 624 WP_182851206.1 type 4a pilus biogenesis protein PilO -
  H7K70_RS26875 (WP8S17C03_52520) pilN 5779499..5780071 (-) 573 WP_182851207.1 type 4a pilus biogenesis protein PilN -
  H7K70_RS26880 (WP8S17C03_52530) pilM 5780071..5781135 (-) 1065 WP_182851208.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 708 a.a.        Molecular weight: 76121.55 Da        Isoelectric Point: 5.2811

>NTDB_id=77163 H7K70_RS26860 WP_182851205.1 5776168..5778294(-) (pilQ) [Metapseudomonas otitidis strain WP8-S17-CRE-03]
MNSSLTRLGLSLLAAMFSPMLLAADLKALDVAALPGGKIELKLGFDGPVVQPRGYTIEQPARIALDLPGVANKLGSKNRE
LGVGNARSVTLVEAKDRTRMIINLSTLVPYSTRVEGNNLYVLVGDAAGAAQQAARTSAPVAAPSQPAAPVKAFAPAGKAI
SNIDFQRGEQGEGNVVITLSDPSVSPNIQEQGGKIRLDFSKTQIPESLRVRLDVKDFATPVQFVSASGSSGDASIVIEPT
GFYDYLAYQTENKLTVSIKPLTQEDMEKRKTERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQ
NVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQISELAPLRRELIQVNYAKAADIAKLFQSVTSGE
PAATDERGSITVDDRTNSIIAYQTQDKLDELRRIVTQLDIPVRQVMIEARIVEANVDYDKALGVRWGGAYTNGNWSSWGK
SGNIGIKDGSGVACGPYNGNCSLPTTASNNPTPFVDMGVSNSTSGIGIGFISSHAILDLQLSAMEKTGNGEIVSQPKVVT
SDKETAKILKGAEIPYQEASSSGATTTSFKEAALSLEVTPQITPDNRIIMEVKVNKDAPDFANQLNGVPPINKNEVNAKV
LVSDGETIVIGGVFSNTQTKAVDKVPFLGDLPFVGRVFRRDVVQDKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2127 bp        

>NTDB_id=77163 H7K70_RS26860 WP_182851205.1 5776168..5778294(-) (pilQ) [Metapseudomonas otitidis strain WP8-S17-CRE-03]
ATGAACAGCTCCCTAACGCGCCTTGGACTTTCTCTTCTGGCAGCCATGTTTTCACCCATGTTGCTGGCTGCTGACCTCAA
GGCTCTGGACGTCGCGGCCCTGCCGGGCGGAAAGATCGAGCTGAAACTCGGTTTCGACGGCCCCGTTGTACAGCCGAGAG
GCTACACGATTGAGCAGCCTGCAAGGATCGCATTGGACTTGCCAGGCGTTGCCAACAAACTTGGCAGCAAGAACCGCGAG
CTCGGGGTAGGTAACGCTCGGAGCGTCACTCTTGTCGAGGCGAAAGATCGCACTCGCATGATCATCAATCTGTCGACTTT
GGTGCCTTACAGCACGCGAGTTGAGGGTAACAACCTTTATGTTCTGGTTGGCGACGCTGCCGGCGCTGCTCAACAGGCTG
CACGCACCAGTGCGCCGGTAGCGGCTCCGTCACAGCCTGCTGCACCCGTCAAAGCGTTCGCGCCTGCGGGCAAGGCAATC
AGCAATATTGATTTCCAGAGAGGCGAGCAGGGCGAAGGTAACGTCGTCATTACCCTATCCGACCCCAGTGTGAGTCCCAA
CATCCAGGAGCAGGGTGGAAAGATCCGCCTGGACTTTTCCAAGACTCAAATCCCGGAGTCTCTGCGTGTTCGTCTTGATG
TAAAGGATTTCGCGACCCCGGTCCAGTTCGTCAGTGCCTCTGGCAGCTCGGGTGATGCCAGTATCGTGATCGAGCCGACG
GGCTTCTACGACTACCTGGCCTACCAGACTGAAAACAAGCTGACTGTCAGCATCAAGCCGCTTACCCAGGAAGATATGGA
GAAGCGCAAGACCGAGCGCTTTGCCTATTCTGGAGAGAAGCTCTCGCTCAATTTCCAGGATATTGATGTCCGCTCGGTAC
TGCAGCTGATCGCTGACTTCACCGATCTGAACCTGGTTGCGTCAGATACCGTACAAGGCAACATCACGCTCCGCCTGCAG
AACGTACCTTGGGATCAGGCACTTGACCTCGTCCTGAAAACCAAGGGCCTGGACAAGCGCAAGGTCGGTAATGTGCTGCT
GGTTGCACCGGCGGACGAGATCGCTGCCCGTGAACGCCAAGAACTGGAGTCTCAGAAGCAGATTTCCGAATTGGCTCCGC
TGCGCCGCGAGCTTATCCAAGTCAATTACGCTAAAGCTGCTGATATCGCCAAGCTCTTTCAATCCGTCACCAGTGGAGAG
CCTGCTGCCACAGATGAGCGTGGTTCTATTACGGTTGATGATCGGACAAATAGTATTATTGCCTATCAAACTCAAGACAA
ACTGGACGAGTTGCGCCGTATTGTTACTCAGCTCGATATTCCTGTCCGCCAAGTAATGATTGAGGCTCGGATTGTTGAGG
CGAATGTCGACTACGACAAGGCCTTAGGCGTAAGGTGGGGTGGGGCTTACACCAATGGGAATTGGAGTAGCTGGGGAAAG
AGTGGCAATATCGGTATTAAGGATGGCAGTGGAGTAGCTTGCGGACCCTACAATGGAAACTGTTCACTGCCTACTACTGC
TAGCAATAATCCAACGCCCTTTGTAGATATGGGTGTCTCCAATAGTACTTCTGGAATTGGTATAGGCTTCATTTCAAGTC
ATGCTATTCTTGATTTGCAGCTCTCTGCAATGGAAAAAACCGGAAATGGAGAGATCGTTTCCCAGCCTAAAGTAGTTACT
TCTGATAAGGAGACGGCAAAGATTCTGAAGGGTGCGGAGATTCCATACCAAGAGGCAAGTTCCAGTGGTGCAACTACCAC
ATCCTTCAAGGAGGCGGCCCTTTCATTGGAGGTAACTCCACAGATTACACCAGATAACCGAATTATCATGGAAGTAAAGG
TCAATAAAGATGCTCCGGATTTTGCTAACCAATTAAATGGCGTGCCCCCAATTAATAAGAATGAGGTTAATGCTAAGGTT
TTGGTTAGTGATGGCGAGACTATTGTAATAGGTGGGGTTTTCTCCAATACACAGACCAAAGCTGTAGACAAGGTACCTTT
CCTTGGTGATCTGCCATTTGTAGGGCGAGTGTTCCGAAGAGATGTTGTTCAAGACAAGAAATCCGAGCTGCTAGTATTCC
TGACTCCGCGTATCATGAACAACCAGGCTATCGCTGTGAGTCGCTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(40-121)

Bacterial type II and III secretion system protein (Secretin)

(543-699)

Secretin and TonB N terminus short domain (STN)

(304-350)

Bacterial type II/III secretion system short domain (Secretin_N)

(378-444)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6S5RWP9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

78.631

100

0.795
  comQ Acinetobacter baylyi ADP1

42.959

99.294

0.427
  pilQ Acinetobacter baumannii strain A118

41.69

100

0.418
  pilQ Acinetobacter baumannii D1279779

41.69

100

0.418
  pilQ Legionella pneumophila strain Lp02

38.144

95.904

0.366
  pilQ Legionella pneumophila strain ERS1305867

38.144

95.904

0.366

Multiple sequence alignment