Detailed information NTG0077164

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: H7K70_RS26880
• Genome accession: NZ_AP022213
• Coordinates: 5780071..5781135 (-)
• Length: 354 a.a.
• NCBI ID: WP_182851208.1
• Uniprot ID: A0A6S5S2F7
• Organism: Metapseudomonas otitidis strain WP8-S17-CRE-03
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 5775071..5786135

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
H7K70_RS26855 (WP8S17C03_52480)	aroK	5775644..5776162 (-)	519	WP_182851204.1	shikimate kinase AroK	-
H7K70_RS26860 (WP8S17C03_52490)	pilQ	5776168..5778294 (-)	2127	WP_182851205.1	type IV pilus secretin PilQ	Machinery gene
H7K70_RS26865 (WP8S17C03_52500)	pilP	5778349..5778879 (-)	531	WP_182852983.1	type 4a pilus biogenesis lipoprotein PilP	-
H7K70_RS26870 (WP8S17C03_52510)	pilO	5778879..5779502 (-)	624	WP_182851206.1	type 4a pilus biogenesis protein PilO	-
H7K70_RS26875 (WP8S17C03_52520)	pilN	5779499..5780071 (-)	573	WP_182851207.1	type 4a pilus biogenesis protein PilN	-
H7K70_RS26880 (WP8S17C03_52530)	pilM	5780071..5781135 (-)	1065	WP_182851208.1	pilus assembly protein PilM	Machinery gene
H7K70_RS26885 (WP8S17C03_52540)	-	5781317..5783740 (+)	2424	WP_394354674.1	penicillin-binding protein 1A	-
H7K70_RS26890 (WP8S17C03_52550)	-	5783846..5785114 (-)	1269	WP_074973678.1	malic enzyme-like NAD(P)-binding protein	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 37720.99 Da
• Isoelectric Point: 4.3980

>NTDB_id=77164 H7K70_RS26880 WP_182851208.1 5780071..5781135(-) (pilM) [Metapseudomonas otitidis strain WP8-S17-CRE-03]
MLGLFNKKANSLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVVAKAKAGSKLAAV
AVAGSAVITKTIEMDAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGVSARNSERMDVLLAACRRENVEVREAALAL
AGLTAKVVDVEAYALERAYGLLASQLGNGSEDLTVAIVDIGATMTTLSVLNNGRTIYSREQLFGGRQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDSEVLQPFKEAVVQQVSRSLQFFFATGQYNDVDYILLAGGTASIPDLDRLIQQKIGTPTLVANP
FADMALSGKVNAGALASDAPALMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=77164 H7K70_RS26880 WP_182851208.1 5780071..5781135(-) (pilM) [Metapseudomonas otitidis strain WP8-S17-CRE-03]
GTGCTAGGGCTTTTCAACAAGAAAGCGAATTCGCTGCTGGGGATCGACATAAGCTCGACCTCGGTCAAGCTCCTCGAATT
GAGTCGCTCGGGTGGCCGCTACAAGGTAGAGGCCTACGCCGTCGAGCCGCTCCCGCCAAATGCTGTCGTCGAAAAGAACA
TTGCTGAGCTGGAAGGCGTCGGCCAGGCACTGTCGCGCGTGGTCGCCAAGGCGAAGGCAGGCTCCAAGCTGGCAGCCGTT
GCTGTGGCGGGTTCAGCCGTCATTACCAAGACGATCGAGATGGATGCAGGGCTGTCCGATGACGAGCTGGAGAACCAGCT
CAAGATCGAAGCTGATCAGTACATCCCTTATCCCCTTGAAGAAGTAGCCATCGATTTCGAGGTTCAGGGCGTTTCTGCAC
GAAACTCCGAGCGAATGGATGTGCTGCTGGCAGCCTGCCGTCGCGAGAACGTTGAAGTGCGCGAGGCAGCGCTTGCTCTC
GCCGGGCTCACCGCGAAAGTCGTGGATGTCGAGGCCTATGCGCTTGAGCGCGCCTATGGGCTCCTTGCTTCCCAGTTGGG
GAATGGATCGGAGGATCTGACGGTAGCGATCGTGGACATCGGCGCGACGATGACCACCCTGAGCGTCTTGAATAATGGTC
GCACCATATACAGTCGCGAGCAGTTGTTCGGAGGTCGCCAGTTGACCGAAGAAATTCAGCGCCGCTATGGCCTGTCTGTC
GAGGAAGCGGGCCTGGCCAAGAAGCAGGGCGGTCTTCCGGACGACTACGACAGCGAGGTTTTGCAACCGTTCAAAGAGGC
AGTTGTCCAGCAGGTCTCGCGGTCGCTGCAGTTCTTTTTTGCAACGGGTCAGTACAACGATGTCGACTACATCCTCCTGG
CTGGCGGGACGGCCTCTATCCCTGATCTGGATCGCCTGATCCAGCAGAAGATCGGAACGCCGACGCTGGTCGCAAACCCC
TTCGCCGACATGGCGCTGAGCGGCAAAGTCAACGCAGGTGCGCTGGCAAGTGATGCGCCGGCCTTGATGATCGCTTGTGG
CTTGGCAATGAGGAGCTTCGACTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A6S5S2F7

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.78	100	0.568
comM	Acinetobacter nosocomialis M2	56.78	100	0.568
comM	Acinetobacter baylyi ADP1	53.955	100	0.54
pilM	Legionella pneumophila strain ERS1305867	48.588	100	0.486