Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   QP018_RS08340 Genome accession   NZ_CP126975
Coordinates   1748236..1749645 (-) Length   469 a.a.
NCBI ID   WP_285092763.1    Uniprot ID   A0AAX3XCK8
Organism   Gallibacterium anatis strain BJF12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1743236..1754645
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP018_RS08305 (QP018_08305) - 1743519..1744178 (+) 660 WP_013746615.1 formate dehydrogenase subunit gamma -
  QP018_RS08310 (QP018_08310) fdhE 1744180..1745103 (+) 924 WP_039094142.1 formate dehydrogenase accessory protein FdhE -
  QP018_RS08315 (QP018_08315) - 1745169..1745441 (-) 273 WP_013746612.1 GNAT family N-acetyltransferase -
  QP018_RS08320 (QP018_08320) yacG 1745528..1745746 (-) 219 WP_013746611.1 DNA gyrase inhibitor YacG -
  QP018_RS08325 (QP018_08325) coaE 1745736..1746368 (-) 633 WP_285092761.1 dephospho-CoA kinase -
  QP018_RS08330 (QP018_08330) - 1746383..1747015 (-) 633 WP_285092762.1 prepilin peptidase -
  QP018_RS08335 (QP018_08335) pilC 1747012..1748220 (-) 1209 WP_013746607.1 type II secretion system F family protein Machinery gene
  QP018_RS08340 (QP018_08340) pilB 1748236..1749645 (-) 1410 WP_285092763.1 GspE/PulE family protein Machinery gene
  QP018_RS08345 (QP018_08345) pilA 1749662..1750123 (-) 462 WP_039148357.1 prepilin peptidase-dependent pilin Machinery gene
  QP018_RS08350 (QP018_08350) ampD 1750228..1750782 (+) 555 WP_285092764.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QP018_RS08355 (QP018_08355) - 1750869..1751204 (+) 336 WP_013746603.1 hypothetical protein -
  QP018_RS08360 (QP018_08360) - 1751313..1752707 (+) 1395 WP_285092765.1 chloride channel protein -
  QP018_RS08365 (QP018_08365) dusA 1752688..1753668 (+) 981 WP_039133907.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 469 a.a.        Molecular weight: 52906.38 Da        Isoelectric Point: 5.3117

>NTDB_id=769625 QP018_RS08340 WP_285092763.1 1748236..1749645(-) (pilB) [Gallibacterium anatis strain BJF12]
MGYCVKALDSEQLFEISELQWQKNQQQYELLLRYLAVPLQEQAQQLWLAVESFDNLAACETFAFLSGKQIEPVAIGQTAL
KQLLQQLQPGTATNQVQESQLHSYQQNDIVAKSDEIAEVDAQEPIIQLFNQLLESGLQYSVSDIHLEPLRQEFQIRFRID
GVLQRHQVLTLSIGQRLNSRLKLLAKLDINELRLPQDGRFRFKTAFGETLDIRLSSLPTQYGEKIVLRLQPNTPVFDDFT
ELGMTNEQAMLFRQALSQPQGLILVTGPTGSGKSLTLYSGLHFLNQESRHILTAEDPIELQLNGIIQTQANVAIGLTFAT
LLRSFLRQDPDVIMVGEIRDQETAEIALRAAQTGHLVLSTLHTNTAIGAISRLQQLGIAQHELENSLLLVVAQRLVRKRC
TLCQGDKSQQCDCVDGYRGRTGVYQLLHYQQQQIQMDYPTLLAAAQLKLQQRITTTAEVERVFGKTENS

Nucleotide


Download         Length: 1410 bp        

>NTDB_id=769625 QP018_RS08340 WP_285092763.1 1748236..1749645(-) (pilB) [Gallibacterium anatis strain BJF12]
ATGGGCTATTGTGTAAAGGCATTGGATTCGGAGCAGCTGTTTGAAATTTCAGAATTGCAATGGCAGAAGAATCAACAGCA
ATATGAACTGTTATTGCGTTATTTGGCGGTACCGTTGCAGGAACAGGCGCAACAGTTATGGTTAGCGGTTGAGTCTTTCG
ATAATTTAGCCGCTTGCGAGACCTTTGCTTTTCTTTCCGGTAAACAGATTGAGCCGGTTGCGATCGGACAAACTGCACTG
AAACAGTTATTACAGCAGTTGCAACCCGGAACGGCGACAAATCAAGTGCAAGAATCACAACTGCATAGTTATCAGCAAAA
TGATATTGTCGCAAAATCGGATGAAATTGCGGAAGTTGATGCACAAGAGCCGATTATTCAACTATTCAATCAATTATTGG
AATCAGGTTTGCAATATTCCGTTTCTGATATTCATCTTGAGCCGTTACGTCAGGAGTTCCAGATCCGCTTTAGAATTGAC
GGCGTATTACAACGGCATCAGGTACTGACGTTGTCAATAGGTCAACGCTTAAACTCACGCCTAAAATTGTTGGCAAAACT
GGATATTAACGAATTACGCTTGCCGCAGGATGGTCGTTTTCGTTTTAAAACCGCATTCGGTGAAACATTAGATATTCGGT
TATCTTCTCTACCGACACAATATGGTGAAAAAATCGTATTACGATTGCAACCTAACACACCGGTGTTTGATGATTTTACC
GAACTGGGAATGACAAACGAGCAGGCTATGCTGTTCCGGCAAGCGTTATCTCAACCGCAAGGATTAATCTTGGTTACCGG
ACCGACCGGAAGTGGTAAAAGTTTGACATTATATAGCGGTTTACATTTTTTAAATCAGGAAAGTCGCCACATTCTTACAG
CGGAAGATCCGATAGAACTGCAATTAAACGGCATTATTCAGACTCAAGCAAATGTCGCAATCGGTTTAACTTTTGCAACA
TTATTGCGGAGTTTTTTACGCCAAGATCCGGATGTCATTATGGTTGGGGAAATTCGAGATCAGGAAACGGCAGAGATAGC
ATTACGCGCGGCACAGACGGGGCATTTAGTGTTATCGACACTGCATACCAATACGGCAATCGGTGCAATTTCCCGATTAC
AACAGTTGGGAATTGCACAACACGAATTGGAAAATTCATTATTGTTGGTGGTGGCACAACGTTTAGTCCGCAAACGTTGC
ACCTTATGCCAAGGTGATAAATCTCAGCAATGCGATTGTGTTGACGGTTATCGCGGACGAACCGGAGTTTATCAACTGTT
ACATTATCAGCAACAACAAATTCAAATGGATTATCCGACATTATTGGCGGCAGCACAGCTTAAATTACAACAACGGATCA
CGACAACAGCAGAGGTTGAGCGCGTCTTCGGCAAGACGGAGAACAGCTAA

Domains

Predicted by InterProScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(26-91)

Type II/IV secretion system protein (T2SSE)

(131-398)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0AAX3XCK8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

59.43

97.228

0.578
  pilB Haemophilus influenzae Rd KW20

59.43

97.228

0.578
  pilB Glaesserella parasuis strain SC1401

53.632

99.787

0.535
  pilB Vibrio parahaemolyticus RIMD 2210633

38.677

100

0.412
  pilB Vibrio cholerae strain A1552

39.834

100

0.409
  pilB Vibrio campbellii strain DS40M4

39.627

100

0.407
  pilB Legionella pneumophila strain ERS1305867

36.157

100

0.373
  pilB Acinetobacter baylyi ADP1

39.503

94.456

0.373
  pilB Acinetobacter baumannii D1279779

39.13

93.177

0.365