Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QP018_RS08345 Genome accession   NZ_CP126975
Coordinates   1749662..1750123 (-) Length   153 a.a.
NCBI ID   WP_039148357.1    Uniprot ID   A0AAX3XBD0
Organism   Gallibacterium anatis strain BJF12     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1744662..1755123
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QP018_RS08315 (QP018_08315) - 1745169..1745441 (-) 273 WP_013746612.1 GNAT family N-acetyltransferase -
  QP018_RS08320 (QP018_08320) yacG 1745528..1745746 (-) 219 WP_013746611.1 DNA gyrase inhibitor YacG -
  QP018_RS08325 (QP018_08325) coaE 1745736..1746368 (-) 633 WP_285092761.1 dephospho-CoA kinase -
  QP018_RS08330 (QP018_08330) - 1746383..1747015 (-) 633 WP_285092762.1 prepilin peptidase -
  QP018_RS08335 (QP018_08335) pilC 1747012..1748220 (-) 1209 WP_013746607.1 type II secretion system F family protein Machinery gene
  QP018_RS08340 (QP018_08340) pilB 1748236..1749645 (-) 1410 WP_285092763.1 GspE/PulE family protein Machinery gene
  QP018_RS08345 (QP018_08345) pilA 1749662..1750123 (-) 462 WP_039148357.1 prepilin peptidase-dependent pilin Machinery gene
  QP018_RS08350 (QP018_08350) ampD 1750228..1750782 (+) 555 WP_285092764.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  QP018_RS08355 (QP018_08355) - 1750869..1751204 (+) 336 WP_013746603.1 hypothetical protein -
  QP018_RS08360 (QP018_08360) - 1751313..1752707 (+) 1395 WP_285092765.1 chloride channel protein -
  QP018_RS08365 (QP018_08365) dusA 1752688..1753668 (+) 981 WP_039133907.1 tRNA dihydrouridine(20/20a) synthase DusA -

Sequence

Protein


Download         Length: 153 a.a.        Molecular weight: 16374.72 Da        Isoelectric Point: 8.0868

>NTDB_id=769626 QP018_RS08345 WP_039148357.1 1749662..1750123(-) (pilA) [Gallibacterium anatis strain BJF12]
MKKREQLKANYAGFTLIELMIVIAIIAVLATIAIPSYQNYTKKAAISELIQASSPYKAEVELCIYNTKSKASCNAGQNGI
QAKPSNMDKFKYLKEVNVAAGVISVSGKSSLENISYTMTPSDGNNGELIDWSVTCSGDNGLFPAGFCSNTSAN

Nucleotide


Download         Length: 462 bp        

>NTDB_id=769626 QP018_RS08345 WP_039148357.1 1749662..1750123(-) (pilA) [Gallibacterium anatis strain BJF12]
ATGAAAAAACGTGAACAACTTAAGGCGAATTATGCCGGATTTACTTTAATCGAATTGATGATTGTCATTGCGATTATTGC
CGTGTTGGCAACCATTGCCATTCCCTCTTATCAAAATTACACCAAAAAGGCGGCAATATCAGAATTGATTCAAGCCTCTT
CACCCTATAAGGCGGAAGTTGAACTCTGTATTTATAACACCAAGAGTAAAGCAAGCTGTAACGCCGGTCAAAACGGCATT
CAGGCGAAGCCGTCTAATATGGATAAATTTAAATATCTTAAAGAGGTTAATGTGGCTGCTGGTGTTATTTCTGTTTCCGG
TAAATCCAGTCTGGAGAACATCAGTTATACGATGACACCGAGTGACGGCAATAACGGTGAATTAATTGATTGGAGTGTAA
CTTGTAGCGGTGATAATGGGCTGTTTCCGGCAGGATTTTGTAGCAATACTTCTGCAAATTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(9-33)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0AAX3XBD0

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Glaesserella parasuis strain SC1401

54

98.039

0.529
  pilA Haemophilus influenzae Rd KW20

52.667

98.039

0.516
  pilA Haemophilus influenzae 86-028NP

56.618

88.889

0.503