Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   PCP85_RS09405 Genome accession   NZ_CP115213
Coordinates   2010752..2012452 (-) Length   566 a.a.
NCBI ID   WP_023092873.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain F063     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2005752..2017452
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PCP85_RS09365 (PCP85_09375) - 2005923..2006348 (+) 426 WP_003094666.1 GNAT family N-acetyltransferase -
  PCP85_RS09370 (PCP85_09380) - 2006345..2006815 (+) 471 WP_003094664.1 hypothetical protein -
  PCP85_RS09375 (PCP85_09385) - 2006812..2007501 (+) 690 WP_003094662.1 energy-coupling factor ABC transporter permease -
  PCP85_RS09380 (PCP85_09390) - 2007607..2007816 (+) 210 WP_003094660.1 hypothetical protein -
  PCP85_RS09385 (PCP85_09395) yacG 2007853..2008053 (-) 201 WP_003094656.1 DNA gyrase inhibitor YacG -
  PCP85_RS09390 (PCP85_09400) coaE 2008050..2008661 (-) 612 WP_003094654.1 dephospho-CoA kinase -
  PCP85_RS09395 (PCP85_09405) pilD 2008658..2009530 (-) 873 WP_023092875.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  PCP85_RS09400 (PCP85_09410) pilC 2009531..2010748 (-) 1218 WP_023092874.1 type II secretion system F family protein Machinery gene
  PCP85_RS09405 (PCP85_09415) pilB 2010752..2012452 (-) 1701 WP_023092873.1 type IV-A pilus assembly ATPase PilB Machinery gene
  PCP85_RS09410 (PCP85_09420) - 2012683..2013147 (+) 465 WP_023092872.1 pilin -
  PCP85_RS09415 (PCP85_09425) - 2013303..2014616 (+) 1314 WP_023980542.1 O-antigen ligase family protein -
  PCP85_RS09425 (PCP85_09435) nadC 2014797..2015645 (-) 849 WP_023092870.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62343.23 Da        Isoelectric Point: 5.5798

>NTDB_id=769399 PCP85_RS09405 WP_023092873.1 2010752..2012452(-) (pilB) [Pseudomonas aeruginosa strain F063]
MNDSTPLSGLAKQLVLNELLDEKTARQAQVQAARNKQSLVSHLVQAKLVQSRALVEVAAEQFGIAYCDLNSLDRESIPKD
VISERLVRQHRVLPLWKRGNKLFIGLSDPTNHQAVTDIQFSTGLTTEALLVEDDKLSVAIEKLYEGAMDALGDLGDIDLD
GVDVGKAESASQDDGGDDAEDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKTYRVRFRTDGMLHEVAKPPIQLASRISARL
KVMAGLDISERRKPQDGRIKMRVSKNKSIDFRVNTLPTLWGEKIVMRILDSSSAQMGIDALGYEEDQKELYLAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTTDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLLNMGVPAFNLATSVNLIIAQRLARKLCSHCKKEHDVPKETLLHEGFPE
DKIGTFKLYSPVGCENCKGGYKGRVGIYEVVKNTPALQRIIMEEGNSIEIAEQARKEGFNDLRTSGLLKAMQGITSLEEV
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=769399 PCP85_RS09405 WP_023092873.1 2010752..2012452(-) (pilB) [Pseudomonas aeruginosa strain F063]
ATGAACGATTCCACGCCTCTCAGCGGCCTTGCCAAGCAACTGGTCCTGAACGAACTCCTCGACGAAAAGACCGCCCGCCA
GGCCCAGGTGCAGGCCGCGCGCAACAAGCAGTCGCTGGTCAGCCACCTGGTCCAGGCCAAGCTGGTGCAGAGCCGGGCGT
TGGTCGAGGTGGCCGCCGAGCAGTTCGGCATCGCCTATTGCGACCTGAACAGCCTGGATCGGGAAAGCATTCCGAAGGAT
GTCATCAGCGAGCGCCTGGTCCGCCAGCACCGCGTGTTGCCGCTGTGGAAACGCGGCAACAAACTGTTCATCGGTCTCTC
CGACCCGACCAACCATCAGGCGGTCACCGATATCCAATTCAGCACCGGCCTCACCACCGAAGCCCTGCTGGTAGAGGACG
ACAAGCTCAGCGTAGCCATCGAGAAGCTTTACGAGGGCGCCATGGACGCCCTCGGCGACCTCGGCGATATCGATCTCGAC
GGGGTCGATGTCGGCAAGGCGGAAAGCGCCAGCCAGGACGACGGCGGCGACGATGCCGAAGATGCCCCCGTGGTGCGCTT
CGTCAACAAGATGCTGCTCGACGCCATCCGCGGCGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTACCGGG
TGCGCTTCCGTACCGACGGCATGCTCCACGAGGTAGCCAAGCCGCCGATCCAGTTGGCCAGCCGCATTTCCGCGCGACTC
AAGGTGATGGCCGGCCTGGATATCTCCGAACGACGCAAGCCGCAGGACGGGCGGATCAAGATGCGCGTGTCGAAGAACAA
GTCGATCGACTTCCGCGTCAACACCCTGCCGACCCTGTGGGGCGAGAAGATCGTGATGCGGATCCTCGACTCCTCCAGCG
CGCAGATGGGCATCGACGCCCTCGGCTACGAAGAGGATCAGAAGGAACTCTATCTGGCTGCGCTCAAACAGCCACAGGGC
ATGATACTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTTTCGCTGTACACCGGCCTGAACATCCTCAATACCACCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACCTGGAAGGCATCAACCAGGTCAACGTCAACCCGCGCCAGG
GCATGGACTTCTCCCAGGCGCTACGCGCCTTCCTGCGCCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTG
GAGACTGCCGAGATCGCCATCAAGGCGGCGCAGACCGGGCATATGGTGATGTCCACCCTGCACACCAACAGCGCCGCCGA
GACCCTGACCCGCCTGCTGAACATGGGCGTGCCGGCATTCAACCTGGCGACCTCGGTGAACCTGATCATCGCCCAGCGCC
TTGCGCGAAAACTCTGTTCGCACTGCAAGAAAGAACACGACGTGCCGAAGGAAACCCTGCTTCACGAGGGCTTCCCGGAA
GACAAGATCGGCACCTTCAAGCTGTATTCGCCTGTAGGCTGCGAAAACTGCAAAGGCGGTTACAAAGGTCGCGTCGGTAT
TTATGAAGTGGTTAAAAACACCCCCGCCCTGCAGCGGATTATCATGGAAGAAGGCAACTCCATCGAGATCGCCGAGCAAG
CCCGCAAAGAAGGCTTCAACGATCTGCGCACCTCAGGCCTGCTGAAAGCCATGCAGGGAATCACCAGCCTGGAGGAAGTC
AACCGCGTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.544

100

0.58
  pilB Acinetobacter baylyi ADP1

56.79

100

0.569
  pilB Legionella pneumophila strain ERS1305867

54.674

100

0.548
  pilB Vibrio cholerae strain A1552

51.264

97.88

0.502
  pilF Neisseria gonorrhoeae MS11

50.177

99.647

0.5
  pilB Vibrio parahaemolyticus RIMD 2210633

52.622

94.346

0.496
  pilB Vibrio campbellii strain DS40M4

51.22

94.17

0.482
  pilB/pilB1 Synechocystis sp. PCC 6803

37.763

100

0.412
  pilF Thermus thermophilus HB27

38.664

100

0.389
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.727

97.173

0.376