Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OZ430_RS16205 Genome accession   NZ_CP115039
Coordinates   3456043..3457746 (-) Length   567 a.a.
NCBI ID   WP_312915417.1    Uniprot ID   -
Organism   Stutzerimonas kunmingensis strain LP1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3451043..3462746
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OZ430_RS16175 (OZ430_16180) - 3451227..3452264 (+) 1038 WP_044315481.1 NADP(H)-dependent aldo-keto reductase -
  OZ430_RS16180 (OZ430_16185) - 3452327..3453007 (+) 681 WP_044315482.1 energy-coupling factor ABC transporter permease -
  OZ430_RS16185 (OZ430_16190) yacG 3453018..3453215 (-) 198 WP_014821358.1 DNA gyrase inhibitor YacG -
  OZ430_RS16190 (OZ430_16195) coaE 3453212..3453820 (-) 609 WP_375664777.1 dephospho-CoA kinase -
  OZ430_RS16195 (OZ430_16200) pilD 3453952..3454821 (-) 870 WP_312915418.1 A24 family peptidase Machinery gene
  OZ430_RS16200 (OZ430_16205) pilC 3454824..3456041 (-) 1218 WP_003302807.1 type II secretion system F family protein Machinery gene
  OZ430_RS16205 (OZ430_16210) pilB 3456043..3457746 (-) 1704 WP_312915417.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OZ430_RS16210 (OZ430_16215) - 3458069..3458563 (+) 495 WP_312915414.1 pilin -
  OZ430_RS16215 (OZ430_16225) - 3459624..3460064 (+) 441 WP_312244535.1 YtoQ family protein -
  OZ430_RS16220 (OZ430_16230) cysN 3460249..3462147 (-) 1899 WP_312915413.1 sulfate adenylyltransferase subunit CysN -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 61900.96 Da        Isoelectric Point: 5.6387

>NTDB_id=768013 OZ430_RS16205 WP_312915417.1 3456043..3457746(-) (pilB) [Stutzerimonas kunmingensis strain LP1]
MSDNISLSGLARQMVQTGVLDEKTARQAQLQAQRSKLSLVTYVVQNKLVKGRAVAELASDQFGVALLDLSALDKESLPKE
LVSEKLARQHRVLPLVKRGTKLYVAISDPANHQSIADIQFSTGLTIESILVEDDKLGAVIDKLYESSTSGLDDLGDVDLG
GIDVESVSDDAKGGEEGEAADDAPVVRFVNKMLLDAIRGGSSDLHFEPYEKAYRVRLRTDGVLHEVARPPIQLAPRISAR
LKVMAGLDISERRKPQDGRIKMKLSKSKAIDFRVNTLPTLWGEKIVMRILDPSSAQMGIDALGYEEDQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTVDVNISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDIIMVGEIRD
LETAEIAIKAAQTGHMVMSTLHTNSAAETLTRLRNMGVAAFNIATSVNLIIAQRLARKLCNSCKKEIELPRDALLEEGFP
ADKIGTFKIYGPVGCDNCKGGYKGRVGIYEVVKNTPALARIIMEDGNSIDISTQMRKDGFNDLRTSALVKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=768013 OZ430_RS16205 WP_312915417.1 3456043..3457746(-) (pilB) [Stutzerimonas kunmingensis strain LP1]
ATGAGCGACAATATCTCCCTTTCCGGCCTGGCCCGACAAATGGTGCAAACCGGAGTGCTAGACGAGAAAACCGCCCGGCA
GGCCCAGCTACAGGCGCAGCGCAGCAAGCTGTCGCTGGTGACCTATGTGGTGCAGAACAAGTTGGTGAAGGGGCGGGCTG
TTGCCGAGCTTGCGTCCGACCAGTTCGGGGTCGCATTGCTGGACTTGTCTGCGCTGGACAAGGAATCCCTACCCAAGGAG
CTGGTCAGTGAGAAGCTGGCGCGCCAGCATCGGGTGCTGCCGTTGGTCAAGCGTGGCACCAAGCTTTATGTCGCCATTTC
CGACCCGGCCAATCATCAGTCGATCGCTGATATCCAGTTCAGCACCGGGTTGACCATCGAGTCGATCTTGGTGGAAGACG
ACAAGCTGGGCGCCGTCATCGACAAGCTTTACGAGAGCAGCACAAGCGGCCTGGACGATCTGGGCGACGTCGATCTGGGC
GGCATCGACGTCGAGTCCGTCAGTGATGATGCCAAAGGCGGAGAGGAGGGTGAGGCCGCCGATGACGCCCCGGTAGTACG
CTTCGTCAATAAGATGCTGCTCGATGCCATACGCGGTGGCTCATCGGACCTACACTTTGAACCCTATGAAAAGGCCTACC
GGGTGCGCCTGCGTACCGATGGGGTGCTGCATGAGGTGGCGCGGCCACCGATCCAGCTGGCTCCGCGTATCTCCGCGCGG
TTGAAAGTGATGGCCGGGCTGGATATTTCCGAGCGGCGCAAGCCGCAGGATGGCCGCATCAAGATGAAGCTGTCCAAGAG
CAAGGCCATCGATTTTCGCGTCAATACGCTACCCACGCTCTGGGGAGAGAAGATCGTGATGCGAATTCTCGATCCGTCCA
GTGCGCAGATGGGCATCGATGCATTGGGTTATGAAGAGGATCAGAAAGAGCTCTATATGAATGCCCTGAAGCAACCACAG
GGCATGATTTTGGTTACCGGCCCGACCGGCTCTGGTAAGACGGTTTCTCTCTACACCGGCCTGAATATTCTCAATACGGT
GGACGTGAATATATCTACTGCCGAAGATCCGGTGGAAATCAACCTCGAGGGCATCAACCAGGTCAACGTCAATCCTCGTC
AGGGGATGGACTTCTCCCAGGCGTTGCGCGCGTTTCTACGTCAGGATCCAGACATCATCATGGTGGGTGAGATCCGCGAT
CTGGAGACCGCTGAGATCGCCATCAAGGCGGCGCAGACGGGCCACATGGTCATGTCGACGCTACACACCAACAGCGCCGC
GGAAACCCTGACCCGTCTTCGCAACATGGGGGTGGCCGCGTTCAATATTGCGACCTCGGTCAACCTGATCATCGCGCAGC
GACTGGCACGCAAGCTTTGCAACAGTTGCAAGAAGGAGATCGAACTGCCGCGCGATGCGCTGTTGGAAGAGGGCTTCCCT
GCCGACAAGATCGGGACGTTCAAAATCTATGGCCCGGTCGGCTGCGACAATTGCAAGGGTGGTTACAAAGGCCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCGGCATTGGCCAGGATTATCATGGAGGACGGCAACTCCATCGACATTTCCACCC
AGATGCGTAAAGACGGCTTCAACGATCTGCGTACTTCGGCTCTTGTAAAAGCCATGCAGGGCGTGACCAGCCTGGAAGAA
GTCAACCGGGTGACCAAGGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

60

99.647

0.598
  pilB Acinetobacter baylyi ADP1

59.22

99.471

0.589
  pilB Legionella pneumophila strain ERS1305867

55.203

100

0.552
  pilB Vibrio cholerae strain A1552

50.906

97.354

0.496
  pilB Vibrio parahaemolyticus RIMD 2210633

48.601

100

0.49
  pilF Neisseria gonorrhoeae MS11

49.201

99.295

0.489
  pilB Vibrio campbellii strain DS40M4

48.415

100

0.485
  pilB/pilB1 Synechocystis sp. PCC 6803

37.013

100

0.402
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

36.134

100

0.379