Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   O6P33_RS03375 Genome accession   NZ_CP114976
Coordinates   702848..704857 (+) Length   669 a.a.
NCBI ID   WP_269818840.1    Uniprot ID   A0AAE9VQ52
Organism   Denitrificimonas caeni strain CE14     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 697848..709857
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O6P33_RS03355 (O6P33_03355) pilM 700013..701077 (+) 1065 WP_269818836.1 pilus assembly protein PilM Machinery gene
  O6P33_RS03360 (O6P33_03360) - 701077..701652 (+) 576 WP_269818837.1 PilN domain-containing protein -
  O6P33_RS03365 (O6P33_03365) - 701649..702272 (+) 624 WP_269818838.1 type 4a pilus biogenesis protein PilO -
  O6P33_RS03370 (O6P33_03370) - 702269..702796 (+) 528 WP_269818839.1 pilus assembly protein PilP -
  O6P33_RS03375 (O6P33_03375) pilQ 702848..704857 (+) 2010 WP_269818840.1 type IV pilus secretin PilQ family protein Machinery gene
  O6P33_RS03380 (O6P33_03380) aroK 704857..705381 (+) 525 WP_269818841.1 shikimate kinase AroK -
  O6P33_RS03385 (O6P33_03385) aroB 705490..706587 (+) 1098 WP_269818842.1 3-dehydroquinate synthase -
  O6P33_RS03390 (O6P33_03390) - 706603..708141 (+) 1539 WP_269818843.1 SPOR domain-containing protein -
  O6P33_RS03395 (O6P33_03395) - 708149..708982 (+) 834 WP_269818844.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
  O6P33_RS03400 (O6P33_03400) - 709006..709641 (-) 636 WP_269818845.1 TetR family transcriptional regulator -

Sequence

Protein


Download         Length: 669 a.a.        Molecular weight: 72307.85 Da        Isoelectric Point: 4.6552

>NTDB_id=767639 O6P33_RS03375 WP_269818840.1 702848..704857(+) (pilQ) [Denitrificimonas caeni strain CE14]
MKTKISRMGITLLAAVFAPALLAANLQGLDVAALPGDKIELKLSFDEPVTAPRGYTIEQPARIALDLPGVKNKLGTKNRE
LGAGNARSVSIVEAQDRTRLIINMTNLAPYSTRADGKDLYVVVGEGVSASEPVANTPIVKPYVAKPRGISNVDFQRGEQG
EGNVVISLNDPTNTPDIQDQGGKIRLTFAKSALPEALRVRLDVKDFATPVQFVNASESNGDVSIVIEPTGIYDYLAYQTD
DQLTISVKPVTQEQIEQRNAERFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVQGNITLRLQNVPWDQALDLV
LQTQGLDKRKIGNVLLVAPADEIAARERQEMEVQKQIAELAPLRRELIQVNYAKASQIAALFQSVTSGNGDQRGSVTVDD
RTNSIIAYQTQEQLDELRRVVAQLDIPVRQVMIEARIVEANVDYSKSLGVRWGGALSTGSGNWTATGQDGAGSPFVDLGA
SNSTSGLGIGFLSNHAILDLQLSAMEKTGNGEVVSQPKVVTSDKETAKILKGAEIPYQEASSSGATSTSFKEAALSLEVT
PQITPDNRIIMDVKVTKDEPDFANALDGVPPIKKNEVNANVLVSDGETIVIGGVFSNTQTKSVDKVPFLGDLPFLGRVFR
RDTVTDQKSELLVFITPRIMNNQAITLNN

Nucleotide


Download         Length: 2010 bp        

>NTDB_id=767639 O6P33_RS03375 WP_269818840.1 702848..704857(+) (pilQ) [Denitrificimonas caeni strain CE14]
ATGAAAACAAAAATTTCTCGTATGGGTATAACTTTGCTGGCTGCTGTATTTGCCCCAGCATTGCTCGCCGCTAACCTGCA
AGGACTTGATGTTGCAGCGTTACCGGGTGATAAAATTGAACTTAAATTATCTTTCGATGAGCCAGTCACTGCCCCGCGAG
GCTATACCATTGAGCAACCTGCGCGTATCGCTTTAGATTTGCCAGGAGTGAAAAATAAGCTCGGTACTAAAAACCGTGAG
CTAGGGGCAGGTAATGCGCGTAGTGTTAGCATTGTTGAAGCGCAAGATCGTACCCGTTTAATCATTAACATGACCAACCT
TGCACCGTACAGCACCCGAGCTGATGGCAAGGACTTATATGTTGTAGTGGGTGAGGGTGTCAGCGCTTCTGAGCCGGTGG
CAAATACCCCTATTGTTAAACCATATGTGGCTAAGCCGCGTGGCATTAGCAATGTTGATTTCCAGCGTGGTGAGCAAGGT
GAAGGTAATGTAGTTATTTCACTTAACGATCCAACCAACACGCCAGATATTCAGGACCAAGGTGGTAAAATTCGTCTCAC
CTTTGCTAAGTCAGCATTGCCTGAAGCGTTACGTGTGCGTTTGGATGTGAAAGATTTTGCCACGCCAGTGCAGTTTGTAA
ATGCCAGCGAGTCCAATGGCGATGTGTCCATTGTGATTGAGCCGACCGGTATTTATGATTACTTGGCCTATCAAACAGAT
GACCAATTAACCATCAGTGTGAAGCCGGTTACGCAAGAGCAAATAGAGCAGCGCAATGCTGAACGTTTTGCTTATTCGGG
TGAGAAACTGTCGTTAAACTTCCAAGACATTGATGTGCGTTCGGTGCTGCAATTAATTGCTGACTTTACCGACTTAAACT
TAGTGGCCAGTGATACCGTGCAAGGCAATATTACTTTGCGCCTGCAGAATGTGCCTTGGGATCAGGCCTTGGATTTAGTG
CTGCAAACTCAGGGGCTCGATAAGCGTAAAATCGGTAATGTGCTTCTGGTTGCGCCGGCAGATGAAATTGCTGCCCGTGA
ACGTCAAGAAATGGAAGTACAGAAGCAAATTGCTGAGCTGGCGCCGCTACGTCGCGAGCTGATCCAAGTGAACTATGCTA
AGGCCTCGCAAATTGCTGCTTTATTCCAGTCAGTGACCAGTGGTAATGGTGATCAGCGTGGTTCAGTCACTGTGGACGAT
CGTACCAACAGCATTATTGCTTATCAGACCCAAGAGCAGCTGGATGAACTTCGCCGAGTGGTTGCTCAGCTGGATATTCC
TGTGCGTCAGGTGATGATTGAAGCGCGCATTGTCGAAGCCAACGTTGATTACAGCAAAAGCTTAGGTGTGCGCTGGGGTG
GAGCTTTAAGCACTGGTAGCGGTAACTGGACGGCAACGGGGCAAGATGGTGCCGGTAGTCCTTTTGTTGACTTAGGCGCG
AGCAATTCAACCTCTGGATTGGGTATTGGTTTCCTCTCCAATCATGCAATTTTAGACTTGCAGTTGTCGGCTATGGAAAA
AACCGGAAACGGTGAGGTAGTTTCCCAGCCTAAAGTGGTTACTTCAGATAAAGAAACTGCCAAAATCTTAAAAGGTGCTG
AGATTCCATACCAAGAAGCCAGCTCCAGTGGTGCAACCAGTACCTCCTTTAAAGAAGCTGCGCTTTCACTTGAAGTTACC
CCGCAAATCACTCCAGATAACCGCATTATCATGGATGTGAAGGTAACTAAGGATGAGCCAGACTTTGCCAATGCTTTAGA
TGGCGTACCGCCAATCAAAAAGAATGAGGTCAACGCTAACGTTTTGGTCTCCGATGGTGAAACTATTGTCATTGGTGGTG
TATTCTCTAATACGCAAACCAAATCGGTTGATAAAGTGCCTTTCTTAGGTGACTTGCCTTTCTTAGGTCGCGTGTTCCGT
CGTGATACTGTGACGGATCAAAAGTCTGAGTTATTGGTCTTTATCACTCCGCGTATTATGAATAATCAGGCCATTACACT
GAATAATTAA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(367-430)

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(504-660)

Secretin and TonB N terminus short domain (STN)

(293-339)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.249

100

0.782
  pilQ Acinetobacter baumannii strain A118

40.432

100

0.42
  pilQ Acinetobacter baumannii D1279779

40.432

100

0.42
  comQ Acinetobacter baylyi ADP1

41.056

100

0.419
  pilQ Legionella pneumophila strain Lp02

38.16

99.103

0.378
  pilQ Legionella pneumophila strain ERS1305867

38.275

98.804

0.378