Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O4546_RS02180 Genome accession   NZ_CP114590
Coordinates   440910..442592 (-) Length   560 a.a.
NCBI ID   WP_077520856.1    Uniprot ID   -
Organism   Salinivibrio kushneri strain TGB19     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 435910..447592
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4546_RS02150 (O4546_02150) mutT 436725..437120 (+) 396 WP_077673503.1 8-oxo-dGTP diphosphatase MutT -
  O4546_RS02155 (O4546_02155) yacG 437163..437363 (-) 201 WP_069363361.1 DNA gyrase inhibitor YacG -
  O4546_RS02160 (O4546_02160) zapD 437374..438114 (-) 741 WP_077456479.1 cell division protein ZapD -
  O4546_RS02165 (O4546_02165) coaE 438138..438752 (-) 615 WP_269587358.1 dephospho-CoA kinase -
  O4546_RS02170 (O4546_02170) pilD 438752..439621 (-) 870 WP_269587359.1 A24 family peptidase Machinery gene
  O4546_RS02175 (O4546_02175) pilC 439686..440900 (-) 1215 WP_077456485.1 type II secretion system F family protein Machinery gene
  O4546_RS02180 (O4546_02180) pilB 440910..442592 (-) 1683 WP_077520856.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O4546_RS02185 (O4546_02185) - 442663..443118 (-) 456 WP_269587360.1 pilin -
  O4546_RS02190 (O4546_02190) nadC 443365..444270 (-) 906 WP_269587361.1 carboxylating nicotinate-nucleotide diphosphorylase -
  O4546_RS02195 (O4546_02195) - 444431..445426 (+) 996 WP_077659064.1 ABC transporter substrate-binding protein -
  O4546_RS02200 (O4546_02200) - 445423..446727 (+) 1305 WP_269587362.1 diguanylate cyclase domain-containing protein -
  O4546_RS02205 (O4546_02205) ampD 446752..447300 (+) 549 WP_077599647.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 61812.90 Da        Isoelectric Point: 6.1655

>NTDB_id=766680 O4546_RS02180 WP_077520856.1 440910..442592(-) (pilB) [Salinivibrio kushneri strain TGB19]
MIPTAQLYQAGLLTDAQRHALGIGDGTPLSVSRLLDAGVDPTALAHTLASQHHLPFSESHQYPFKDTCYALDRRDLIQRH
QVLPLAQQGQELLLGVSDPDNTEAVADFRFSTGLNVRLVVLSQPDLDAALRHLYGADIDGTGDGITISDQDLAALTVTDS
PQEDSAELQSDQAPISRYIQQILVDAVRKNASDVHFEPFEHHYQIRFRQDGLLHTYAHPPAGVSRRLSTRLKILAQLNIA
ERRLPQDGRIKLTLTADHTVDMRVSTLPTVWGEKVVVRLLNNGQQQLTLHDLGLTEWQRQHYQDALDRPQGMVLFTGPTG
SGKTVSLYSGLRYLNQPQRNIATVEDPVEIQLEGINQVNVNPSIGLDFATALRAFLRQDPDVVMVGEIRDLDTAEIGIKA
AQTGHLVLSTLHTNSAAESITRLSHMGIAPYNLASSLTLVVAQRLVRRLCPVCKQPHPSDDPRLQYLANLYPSLTPSTLY
QANPHGCYQCYQGYQGRTGVYEVLPVTEKITSAIYRNASAVELTQLATEEGMWRLAESGVAKLNQGITSIQELTRVIQLA

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=766680 O4546_RS02180 WP_077520856.1 440910..442592(-) (pilB) [Salinivibrio kushneri strain TGB19]
ATGATCCCAACCGCTCAGTTGTATCAAGCCGGTTTATTGACCGATGCCCAGCGTCATGCGTTGGGCATCGGCGATGGCAC
ACCGCTTAGCGTCTCCCGTTTACTCGATGCCGGGGTTGACCCCACCGCGCTCGCCCACACCCTCGCGAGTCAGCATCACC
TGCCTTTTTCGGAAAGCCATCAGTACCCGTTTAAAGACACCTGTTATGCCCTAGATAGGCGCGATTTGATTCAGCGTCAT
CAAGTGCTGCCGCTGGCCCAGCAAGGCCAAGAGCTACTGCTCGGCGTCAGTGACCCAGATAACACAGAGGCGGTCGCCGA
CTTTCGCTTTTCTACTGGGCTCAATGTCCGTTTAGTGGTGCTCAGTCAGCCGGATTTGGATGCCGCGCTGCGTCACTTGT
ACGGCGCAGATATCGATGGCACCGGCGATGGCATCACCATCAGTGATCAAGACTTAGCGGCCCTGACCGTCACCGATAGC
CCACAAGAAGACAGCGCAGAGCTACAAAGTGATCAGGCGCCGATCAGTCGTTACATTCAACAGATATTGGTAGATGCGGT
GCGTAAAAACGCCTCCGACGTACACTTCGAGCCCTTTGAACATCACTACCAAATTCGCTTTCGCCAAGACGGTTTATTGC
ACACCTATGCCCACCCTCCTGCGGGGGTCTCGCGGCGGCTGTCGACTCGACTCAAAATCTTGGCCCAACTCAATATTGCC
GAACGACGTTTACCACAAGACGGGCGCATTAAGCTGACCCTCACTGCCGATCACACCGTCGATATGCGCGTATCCACCTT
ACCGACCGTTTGGGGCGAGAAGGTGGTGGTTCGCTTGTTGAATAATGGCCAGCAGCAACTGACGCTACATGATTTAGGCT
TAACCGAGTGGCAGCGCCAGCACTATCAAGACGCATTGGATAGGCCGCAAGGGATGGTTTTGTTTACCGGCCCAACGGGA
AGCGGCAAAACCGTCTCTTTGTATAGTGGGCTCAGGTATCTCAATCAGCCGCAGCGTAATATTGCCACCGTCGAAGATCC
GGTTGAGATCCAGCTAGAAGGCATCAATCAAGTCAATGTTAATCCTAGCATCGGTTTAGATTTCGCCACCGCACTCCGTG
CCTTTTTGCGCCAAGATCCCGATGTGGTGATGGTGGGAGAAATACGCGATCTCGACACCGCTGAGATCGGGATCAAGGCC
GCGCAAACCGGTCATCTGGTGTTATCAACGCTGCATACCAATTCGGCGGCCGAAAGCATCACCCGTTTATCCCATATGGG
CATTGCGCCCTACAACCTTGCCAGCTCTCTAACTTTGGTGGTGGCTCAGCGTTTGGTACGTCGTTTATGCCCTGTTTGTA
AACAGCCGCACCCGTCTGATGACCCACGCCTTCAGTACTTAGCCAACCTCTATCCAAGCTTAACCCCAAGCACGCTCTAC
CAAGCCAATCCCCACGGCTGTTACCAGTGCTATCAGGGTTATCAAGGACGGACTGGGGTGTACGAAGTGCTCCCCGTGAC
AGAAAAGATAACCAGCGCCATTTACCGAAATGCCAGCGCGGTGGAACTCACTCAACTGGCCACCGAAGAAGGTATGTGGC
GATTGGCCGAATCTGGGGTTGCCAAACTCAACCAAGGGATCACCAGCATCCAAGAGCTTACCCGCGTTATTCAGCTTGCC
TAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(181-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(9-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

53.777

99.286

0.534
  pilB Vibrio campbellii strain DS40M4

53.058

99.286

0.527
  pilB Vibrio cholerae strain A1552

51.872

100

0.52
  pilB Acinetobacter baumannii D1279779

46.463

93.393

0.434
  pilB Legionella pneumophila strain ERS1305867

46.731

92.857

0.434
  pilB Acinetobacter baylyi ADP1

46.507

89.464

0.416
  pilF Neisseria gonorrhoeae MS11

42.748

93.571

0.4
  pilF Thermus thermophilus HB27

39.238

93.75

0.368