Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N8M53_RS02040 Genome accession   NZ_CP114588
Coordinates   403160..404842 (-) Length   560 a.a.
NCBI ID   WP_269579292.1    Uniprot ID   A0AA47LRR3
Organism   Salinivibrio kushneri strain TGB11     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 398160..409842
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N8M53_RS02010 (N8M53_02010) mutT 398968..399363 (+) 396 WP_269579286.1 8-oxo-dGTP diphosphatase MutT -
  N8M53_RS02015 (N8M53_02015) yacG 399412..399612 (-) 201 WP_269579287.1 DNA gyrase inhibitor YacG -
  N8M53_RS02020 (N8M53_02020) zapD 399623..400363 (-) 741 WP_269579288.1 cell division protein ZapD -
  N8M53_RS02025 (N8M53_02025) coaE 400388..401002 (-) 615 WP_269579289.1 dephospho-CoA kinase -
  N8M53_RS02030 (N8M53_02030) pilD 401002..401871 (-) 870 WP_269579290.1 prepilin peptidase Machinery gene
  N8M53_RS02035 (N8M53_02035) pilC 401936..403150 (-) 1215 WP_269579291.1 type II secretion system F family protein Machinery gene
  N8M53_RS02040 (N8M53_02040) pilB 403160..404842 (-) 1683 WP_269579292.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N8M53_RS02045 (N8M53_02045) - 404850..405299 (-) 450 WP_269579293.1 pilin -
  N8M53_RS02050 (N8M53_02050) nadC 405547..406452 (-) 906 WP_269579294.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N8M53_RS02055 (N8M53_02055) - 406612..407607 (+) 996 WP_269579295.1 ABC transporter substrate-binding protein -
  N8M53_RS02060 (N8M53_02060) - 407604..408908 (+) 1305 WP_269579296.1 diguanylate cyclase domain-containing protein -
  N8M53_RS02065 (N8M53_02065) ampD 408933..409481 (+) 549 WP_269579297.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 61949.18 Da        Isoelectric Point: 5.9123

>NTDB_id=766649 N8M53_RS02040 WP_269579292.1 403160..404842(-) (pilB) [Salinivibrio kushneri strain TGB11]
MIPTAPLYQAGLLTDAQRDMLGIGDNTSLSLAHILDAGVDPTALAHTLASQHHLPFSESHQYPYKDTCYALDRRDLIQRH
QVLPLAQQGQELLLGVSDPDNQEAVADFRFSTGLNVRLVVLSQPDLDAALRHLYGADIDGTGDGMTISDQDLAALTVTDS
PQEDSVELQSDQAPISRYIQQILVDAVRKKASDVHFEPFEHHYRIRFRQDGLLHTYAHPPAGVSRRLSTRLKILAQLNIA
ERRLPQDGRIKLTLTADHTVDMRVSTLPTIWGEKVVVRLLDNGQQQLTLHDLGLTEWQRQRYQDALDRPQGMVLFTGPTG
SGKTVSLYSGLRYLNQPERNIATVEDPVEIQLEGINQVNVNPNIGLDFATALRAFLRQDPDVVMVGEIRDLDTAEIGIKA
AQTGHLVLSTLHTNSAAESITRLAHMGIAPYNLASSLTLVVAQRLVRCLCPICKRPHPSDDPRLQYLANLYPSLTPSTLY
QANSQGCYQCYQGYQGRTGVYEVLPVTEKVASAIYRNASAIELTQLATEEGMWRLADSGVAKLNQGITSIQELTRVIQLA

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=766649 N8M53_RS02040 WP_269579292.1 403160..404842(-) (pilB) [Salinivibrio kushneri strain TGB11]
ATGATCCCAACCGCTCCGTTGTATCAAGCCGGTTTATTGACTGATGCCCAGCGCGATATGCTGGGCATTGGCGACAATAC
CTCGCTCAGTCTTGCGCATATACTCGATGCTGGGGTTGACCCCACCGCGCTCGCCCACACCCTTGCGAGTCAGCATCACC
TGCCTTTTTCGGAAAGCCATCAGTACCCGTATAAAGACACCTGTTATGCCCTAGATAGGCGCGATTTGATTCAGCGTCAC
CAAGTGTTGCCGCTTGCCCAGCAAGGCCAAGAGCTATTACTGGGGGTCAGTGATCCAGATAACCAAGAAGCGGTCGCCGA
CTTTCGCTTTTCTACTGGGCTCAATGTCCGGTTAGTGGTGCTTAGTCAGCCGGATTTGGATGCCGCTTTGCGTCATTTAT
ATGGTGCAGATATCGATGGCACGGGCGATGGCATGACCATCAGTGATCAGGACTTAGCGGCCCTAACCGTCACCGATAGC
CCACAAGAAGACAGTGTCGAGTTACAAAGCGATCAGGCGCCCATCAGTCGTTATATTCAACAGATCTTGGTGGATGCAGT
GCGCAAAAAAGCCTCCGACGTGCACTTCGAGCCCTTTGAGCATCACTATCGAATTCGCTTTCGCCAAGACGGTTTATTAC
ACACCTATGCTCACCCTCCAGCGGGGGTCTCGCGGCGGCTGTCGACCCGACTCAAAATCTTAGCCCAACTCAATATTGCC
GAACGACGTTTGCCACAAGACGGGCGCATTAAGCTGACCCTCACCGCCGATCATACGGTCGATATGCGCGTCTCCACCTT
GCCAACCATTTGGGGTGAGAAAGTAGTCGTTCGTCTGTTAGATAATGGTCAGCAGCAATTGACGCTGCATGATTTAGGCT
TAACTGAGTGGCAACGCCAGCGCTATCAAGACGCATTGGATAGGCCGCAAGGGATGGTTTTATTTACCGGCCCGACGGGA
AGTGGCAAAACCGTTTCTTTGTATAGTGGGCTACGGTACCTCAATCAACCTGAGCGCAATATTGCCACCGTCGAAGATCC
GGTCGAGATCCAGCTAGAAGGCATCAATCAAGTCAATGTTAATCCTAACATCGGTTTAGATTTCGCCACCGCACTCCGCG
CTTTTTTGCGCCAAGATCCCGATGTGGTGATGGTAGGAGAAATACGCGACCTCGACACCGCTGAAATCGGGATCAAGGCC
GCGCAAACCGGTCACCTAGTATTATCGACGCTGCATACTAATTCTGCGGCCGAAAGCATCACCCGTTTAGCCCATATGGG
CATTGCGCCCTACAATCTTGCCAGTTCACTGACCTTGGTGGTGGCTCAGCGCTTGGTACGCTGTTTATGCCCGATTTGTA
AACGGCCGCACCCGTCTGATGACCCACGTCTTCAGTACTTAGCCAACCTCTACCCAAGCTTAACCCCAAGCACCCTGTAC
CAAGCTAACTCTCAAGGCTGTTACCAGTGCTATCAAGGCTATCAAGGGCGTACTGGTGTGTACGAAGTGCTCCCCGTAAC
AGAAAAGGTTGCCAGCGCCATTTACCGAAATGCCAGCGCGATTGAGCTAACCCAACTGGCTACCGAAGAAGGCATGTGGC
GATTGGCCGACTCTGGGGTTGCCAAACTCAACCAAGGGATCACCAGCATCCAAGAGCTTACCCGCGTTATTCAGCTTGCC
TAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(9-136)

Type II/IV secretion system protein (T2SSE)

(181-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

54.137

99.286

0.538
  pilB Vibrio campbellii strain DS40M4

53.237

99.286

0.529
  pilB Vibrio cholerae strain A1552

54.335

92.679

0.504
  pilB Legionella pneumophila strain ERS1305867

47.308

92.857

0.439
  pilB Acinetobacter baumannii D1279779

46.023

94.286

0.434
  pilB Acinetobacter baylyi ADP1

47.433

86.964

0.412
  pilF Neisseria gonorrhoeae MS11

42.453

94.643

0.402
  pilB/pilB1 Synechocystis sp. PCC 6803

38.846

92.857

0.361
  pilF Thermus thermophilus HB27

38.476

93.75

0.361