Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O4598_RS02090 Genome accession   NZ_CP114586
Coordinates   413340..415022 (-) Length   560 a.a.
NCBI ID   WP_102505690.1    Uniprot ID   -
Organism   Salinivibrio kushneri strain TGB4     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 408340..420022
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4598_RS02060 (O4598_02060) mutT 409155..409550 (+) 396 WP_269599977.1 8-oxo-dGTP diphosphatase MutT -
  O4598_RS02065 (O4598_02065) yacG 409593..409793 (-) 201 WP_069363361.1 DNA gyrase inhibitor YacG -
  O4598_RS02070 (O4598_02070) zapD 409804..410544 (-) 741 WP_077456479.1 cell division protein ZapD -
  O4598_RS02075 (O4598_02075) coaE 410568..411182 (-) 615 WP_116644578.1 dephospho-CoA kinase -
  O4598_RS02080 (O4598_02080) pilD 411182..412051 (-) 870 WP_269599978.1 prepilin peptidase Machinery gene
  O4598_RS02085 (O4598_02085) pilC 412116..413330 (-) 1215 WP_102505689.1 type II secretion system F family protein Machinery gene
  O4598_RS02090 (O4598_02090) pilB 413340..415022 (-) 1683 WP_102505690.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O4598_RS02095 (O4598_02095) - 415093..415503 (-) 411 WP_102505691.1 pilin -
  O4598_RS02100 (O4598_02100) nadC 415750..416655 (-) 906 WP_269599981.1 carboxylating nicotinate-nucleotide diphosphorylase -
  O4598_RS02105 (O4598_02105) - 416816..417811 (+) 996 WP_077659064.1 ABC transporter substrate-binding protein -
  O4598_RS02110 (O4598_02110) - 417808..419112 (+) 1305 WP_269599982.1 sensor domain-containing diguanylate cyclase -
  O4598_RS02115 (O4598_02115) ampD 419137..419685 (+) 549 WP_077599647.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 61752.89 Da        Isoelectric Point: 6.2533

>NTDB_id=766620 O4598_RS02090 WP_102505690.1 413340..415022(-) (pilB) [Salinivibrio kushneri strain TGB4]
MIPTAQLYQAGLLTDAQRHALGIGDGTPLSVSRLLDVGVDPTALAHTLASQHHLPFSESHQYPFKDTCYALDRRDLIQRH
QVLPLAQQGQELLLGVSDPDNTEAVADFRFSTGLNVRLVVLSQPDLDAALRHLYGADIDGTGDGITISDQDLAALTVTDS
PQEDSAELQSDQAPISRYIQQILVDAVRKNASDVHFEPFEHHYQIRFRQDGLLHTYAHPPAGVSRRLSTRLKILAQLNIA
ERRLPQDGRIKLTLTADHTVDMRVSTLPTVWGEKVVVRLLNNGQQQLTLHDLGLTEWQRQHYQDALDRPQGMVLFTGPTG
SGKTVSLYSGLRYLNQPQRNIATVEDPVEIQLEGINQVNVNPSIGLDFATALRAFLRQDPDVVMVGEIRDLDTAEIGIKA
AQTGHLVLSTLHTNSAAESITRLSHMGIAPYNLASSLTLVVAQRLVRRLCPVCKQPHPSDDPRLQYLANLYPSLTPSTLY
QANPHGCYQCYQGYQGRTGVYEVLPVTEKIASAIYRNASAVALTQLATEEGMWRLAESGVAKLNQGITSIQELTRVIQLA

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=766620 O4598_RS02090 WP_102505690.1 413340..415022(-) (pilB) [Salinivibrio kushneri strain TGB4]
ATGATCCCAACCGCTCAGTTGTATCAAGCCGGTTTATTGACCGATGCCCAGCGTCATGCGTTGGGCATTGGCGATGGCAC
ACCGCTTAGCGTCTCCCGTTTACTCGATGTCGGGGTTGACCCCACCGCGCTCGCCCACACCCTCGCGAGTCAGCATCACC
TGCCTTTTTCGGAAAGCCATCAGTACCCCTTTAAAGACACCTGTTATGCCCTAGATAGGCGCGATTTGATTCAGCGCCAT
CAAGTGTTGCCGCTGGCCCAGCAAGGCCAAGAGCTACTGCTCGGCGTCAGTGACCCAGATAACACAGAGGCGGTCGCCGA
CTTTCGCTTTTCTACTGGGCTCAATGTCCGTTTAGTGGTGCTCAGTCAGCCGGATTTGGATGCCGCGCTGCGTCACTTAT
ACGGCGCAGATATCGATGGCACCGGCGATGGCATCACCATCAGTGATCAAGACTTAGCCGCCCTGACCGTCACCGATAGC
CCACAAGAAGACAGCGCTGAGCTACAAAGCGATCAGGCGCCGATCAGTCGTTACATTCAACAGATATTGGTGGATGCGGT
GCGTAAAAACGCCTCCGACGTGCACTTCGAGCCCTTTGAACATCACTACCAAATTCGCTTTCGCCAAGACGGTTTATTGC
ACACCTATGCCCACCCTCCTGCGGGGGTCTCGCGGCGGCTGTCGACTCGACTCAAAATCCTGGCCCAACTCAATATTGCC
GAACGACGTTTACCACAAGACGGGCGCATTAAGCTGACCCTCACTGCCGATCACACCGTCGATATGCGCGTATCCACCTT
ACCGACCGTTTGGGGCGAGAAGGTGGTGGTTCGCTTGTTGAATAATGGCCAGCAGCAACTGACGCTACATGATTTAGGCT
TAACCGAGTGGCAGCGCCAGCACTATCAAGACGCATTGGATAGGCCGCAAGGGATGGTTTTGTTTACCGGCCCAACGGGA
AGCGGCAAAACCGTCTCTTTGTATAGTGGGCTTAGGTATCTCAACCAGCCGCAGCGTAATATTGCCACCGTCGAAGATCC
GGTTGAGATCCAGCTAGAAGGCATCAATCAAGTCAATGTTAATCCCAGCATCGGTTTAGATTTCGCCACCGCGCTCCGTG
CCTTTTTGCGCCAAGATCCCGATGTGGTGATGGTGGGAGAAATACGCGATCTCGACACCGCTGAAATCGGGATCAAGGCC
GCGCAAACCGGTCATCTGGTGTTATCAACGCTGCATACCAATTCGGCGGCCGAAAGCATCACCCGTTTATCCCACATGGG
CATTGCCCCCTACAATCTTGCCAGCTCACTGACCTTGGTGGTGGCTCAGCGTTTGGTACGCCGTTTATGCCCTGTTTGTA
AACAGCCGCACCCGTCTGATGACCCACGCCTTCAGTACTTAGCCAACCTCTATCCAAGCTTAACCCCAAGCACGCTCTAC
CAAGCTAATCCCCACGGCTGTTACCAGTGCTATCAGGGTTATCAAGGGCGGACTGGGGTGTACGAAGTGCTCCCCGTGAC
AGAAAAGATTGCCAGCGCCATTTACCGAAATGCCAGCGCGGTGGCACTCACTCAACTGGCCACCGAAGAAGGTATGTGGC
GATTGGCCGAGTCTGGGGTTGCCAAACTCAACCAAGGGATCACCAGCATCCAAGAGCTTACCCGCGTTATTCAGCTTGCC
TAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(9-136)

Type II/IV secretion system protein (T2SSE)

(181-448)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

53.597

99.286

0.532
  pilB Vibrio campbellii strain DS40M4

53.131

99.821

0.53
  pilB Vibrio cholerae strain A1552

54.44

92.5

0.504
  pilB Acinetobacter baumannii D1279779

46.198

93.929

0.434
  pilB Legionella pneumophila strain ERS1305867

46.731

92.857

0.434
  pilB Acinetobacter baylyi ADP1

46.307

89.464

0.414
  pilF Neisseria gonorrhoeae MS11

42.802

93.036

0.398
  pilF Thermus thermophilus HB27

39.048

93.75

0.366