Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   N7E60_RS02330 Genome accession   NZ_CP114584
Coordinates   462877..464559 (-) Length   560 a.a.
NCBI ID   WP_269598012.1    Uniprot ID   -
Organism   Salinivibrio proteolyticus strain TGB10     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 457877..469559
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  N7E60_RS02300 (N7E60_02300) mutT 458704..459099 (+) 396 WP_069586607.1 8-oxo-dGTP diphosphatase MutT -
  N7E60_RS02305 (N7E60_02305) yacG 459130..459336 (-) 207 WP_069586608.1 DNA gyrase inhibitor YacG -
  N7E60_RS02310 (N7E60_02310) zapD 459341..460081 (-) 741 WP_269598008.1 cell division protein ZapD -
  N7E60_RS02315 (N7E60_02315) coaE 460105..460719 (-) 615 WP_269598009.1 dephospho-CoA kinase -
  N7E60_RS02320 (N7E60_02320) pilD 460719..461588 (-) 870 WP_269598010.1 A24 family peptidase Machinery gene
  N7E60_RS02325 (N7E60_02325) pilC 461653..462867 (-) 1215 WP_269598011.1 type II secretion system F family protein Machinery gene
  N7E60_RS02330 (N7E60_02330) pilB 462877..464559 (-) 1683 WP_269598012.1 type IV-A pilus assembly ATPase PilB Machinery gene
  N7E60_RS02335 (N7E60_02335) - 464584..464997 (-) 414 WP_269598013.1 pilin -
  N7E60_RS02340 (N7E60_02340) nadC 465248..466156 (-) 909 WP_269598015.1 carboxylating nicotinate-nucleotide diphosphorylase -
  N7E60_RS02345 (N7E60_02345) - 466319..467311 (+) 993 WP_269598017.1 ABC transporter substrate-binding protein -
  N7E60_RS02350 (N7E60_02350) - 467308..468612 (+) 1305 WP_269598018.1 diguanylate cyclase domain-containing protein -
  N7E60_RS02355 (N7E60_02355) ampD 468640..469194 (+) 555 WP_269598019.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -

Sequence

Protein


Download         Length: 560 a.a.        Molecular weight: 61821.03 Da        Isoelectric Point: 5.7358

>NTDB_id=766590 N7E60_RS02330 WP_269598012.1 462877..464559(-) (pilB) [Salinivibrio proteolyticus strain TGB10]
MIPTAQLYQAGLLTDAQRDMLGIGDNTSLNLAHILDAGVDPTALANTLATLHHLPFAESHQYPFKDTCYALDRRDLIQRH
QVLPLAQQGQELLLGVSDPDNAEAVADFRFSTGLNVRLVVLSQPDLDAALRHLYGADIDGTGDGITISDQDLAALTVTDS
PQEDSAELQSDQAPISRYIQQILVDAVRKKASDVHFEPFEHHYQIRFRQDGLLQTYAHPPAGVSRRLSTRLKILAQLNIA
ERRLPQDGRIKLTLTADHTVDMRVSTLPTIWGEKVVVRLLDNGQQQLTLHDLGLTEWQRQHYQDALDRPQGMILFTGPTG
SGKTVSLYSGLKYLNQPERNIATVEDPVEIQLEGVNQVNVNTAIGLDFATALRAFLRQDPDVVMVGEIRDLDTAEIGIKA
AQTGHLVLSTLHTNSAAESITRLSHMGIAPYNLASSLTLVVAQRLVRRLCPMCKQPHPADDPHLQHLANLYPTLTPNTLY
QAHPHGCYQCYQGYQGRTGVYEVLPVTETIASAIYRNASAIELTQLATEEGMWRLAESGIAKLNQGITSIQELTRVIQLA

Nucleotide


Download         Length: 1683 bp        

>NTDB_id=766590 N7E60_RS02330 WP_269598012.1 462877..464559(-) (pilB) [Salinivibrio proteolyticus strain TGB10]
ATGATCCCAACCGCTCAGTTGTATCAAGCCGGTTTATTGACTGATGCCCAGCGCGATATGCTGGGCATTGGCGACAATAC
TTCGCTCAATCTTGCGCATATACTCGATGCTGGGGTTGACCCCACCGCGCTCGCCAACACCCTCGCCACGCTGCACCATC
TTCCGTTCGCAGAGAGCCATCAGTACCCGTTTAAAGACACCTGCTATGCCCTCGACCGGCGTGATTTAATTCAGCGTCAC
CAAGTGTTACCGCTTGCTCAGCAAGGCCAAGAGCTATTACTGGGGGTCAGTGATCCAGATAACGCAGAAGCTGTCGCCGA
CTTTCGCTTTTCTACTGGGCTCAATGTCCGGTTAGTGGTGCTCAGTCAGCCAGATTTGGATGCTGCGTTACGCCACTTAT
ACGGGGCAGATATCGATGGCACTGGCGATGGTATCACCATCAGTGATCAGGACTTGGCGGCCCTGACCGTCACCGATAGC
CCACAAGAGGACAGTGCCGAGCTACAAAGCGATCAGGCGCCCATCAGTCGTTACATTCAACAGATCTTAGTGGATGCGGT
GCGCAAAAAAGCCTCCGACGTGCATTTCGAGCCTTTTGAGCATCACTACCAAATTCGTTTTCGCCAAGACGGTTTATTAC
AAACCTATGCTCATCCTCCGGCAGGGGTCTCGCGGCGACTGTCGACCCGGCTCAAAATCTTAGCCCAACTCAATATTGCC
GAACGACGTTTGCCACAGGACGGGCGCATCAAGCTGACCCTCACCGCCGACCATACCGTCGATATGCGCGTCTCTACCCT
GCCAACCATTTGGGGCGAGAAAGTAGTGGTTCGTCTGTTGGATAATGGCCAGCAGCAATTAACCTTGCATGATTTAGGCT
TAACCGAGTGGCAACGCCAGCACTATCAAGACGCATTAGATAGACCGCAAGGCATGATTTTGTTTACCGGCCCAACCGGA
AGCGGCAAAACCGTCTCCTTGTACAGTGGGTTAAAGTATCTCAATCAGCCCGAGCGTAATATTGCCACCGTCGAAGATCC
GGTTGAGATCCAGCTAGAAGGCGTCAACCAAGTCAACGTTAATACGGCCATTGGTTTAGATTTTGCCACCGCACTCCGTG
CATTTTTGCGCCAAGATCCCGATGTAGTCATGGTGGGGGAAATTCGCGATCTCGACACCGCTGAAATCGGGATCAAGGCC
GCGCAAACTGGTCATCTGGTGTTATCAACCCTGCATACCAATTCCGCCGCCGAAAGCATCACCCGTTTGTCCCATATGGG
CATTGCCCCCTACAACCTTGCCAGCTCCCTAACTTTGGTGGTGGCCCAACGTTTAGTCCGCCGTTTATGCCCGATGTGTA
AACAGCCGCATCCAGCTGATGATCCGCACCTTCAGCACTTAGCTAACCTCTATCCGACGTTAACCCCAAACACCTTGTAC
CAAGCTCATCCTCACGGCTGCTATCAGTGTTATCAAGGCTATCAGGGTCGCACAGGGGTGTATGAGGTGCTTCCTGTCAC
CGAGACTATTGCCAGCGCCATTTACCGCAATGCCAGTGCCATCGAACTGACCCAACTGGCGACAGAGGAAGGGATGTGGC
GATTGGCCGAATCCGGGATTGCCAAACTCAATCAAGGGATCACCAGCATTCAAGAGCTTACTCGCGTTATTCAGCTTGCC
TAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(181-448)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(9-136)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio parahaemolyticus RIMD 2210633

53.957

99.286

0.536
  pilB Vibrio campbellii strain DS40M4

52.743

100

0.532
  pilB Vibrio cholerae strain A1552

53.861

92.5

0.498
  pilB Acinetobacter baumannii D1279779

45.817

93.929

0.43
  pilB Legionella pneumophila strain ERS1305867

48.975

87.143

0.427
  pilB Acinetobacter baylyi ADP1

46.507

89.464

0.416
  pilF Neisseria gonorrhoeae MS11

42.095

93.75

0.395
  pilF Thermus thermophilus HB27

38.857

93.75

0.364