Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   O4444_RS10570 Genome accession   NZ_CP114549
Coordinates   2314187..2315920 (+) Length   577 a.a.
NCBI ID   WP_010894965.1    Uniprot ID   -
Organism   Xylella fastidiosa subsp. pauca strain ALM4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2309187..2320920
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  O4444_RS10550 (O4444_10550) pilC 2309530..2310732 (-) 1203 WP_010894959.1 type II secretion system F family protein Machinery gene
  O4444_RS10555 (O4444_10555) - 2311175..2311621 (+) 447 WP_282459591.1 pilin -
  O4444_RS10560 (O4444_10560) - 2312925..2313371 (+) 447 WP_282459592.1 pilin -
  O4444_RS10565 (O4444_10565) - 2313805..2314035 (-) 231 WP_282459593.1 hypothetical protein -
  O4444_RS10570 (O4444_10570) pilB 2314187..2315920 (+) 1734 WP_010894965.1 type IV-A pilus assembly ATPase PilB Machinery gene
  O4444_RS10575 (O4444_10575) pilR 2316014..2317426 (-) 1413 WP_031338065.1 sigma-54 dependent transcriptional regulator Regulator
  O4444_RS10580 (O4444_10580) - 2317612..2319222 (-) 1611 WP_023907063.1 ATP-binding protein -
  O4444_RS10585 (O4444_10585) sucC 2319472..2320635 (+) 1164 WP_010894968.1 ADP-forming succinate--CoA ligase subunit beta -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62964.01 Da        Isoelectric Point: 5.9154

>NTDB_id=766344 O4444_RS10570 WP_010894965.1 2314187..2315920(+) (pilB) [Xylella fastidiosa subsp. pauca strain ALM4]
MNTPHSANLVGITGIARRLVQDGSIEEATARTAQNQAMAAKVPLPQWLIDKKLVSTAKLAAANALEFGMPLLDVSAFDHN
HNAMSLVDEELLQKHRVLPLFKRGNRLFVGISNPTQTQALDDIKFHTNLVVEPILVDEDQIRRTLEQWQSRSGALSSALE
EEDNGIEGLDIGGGDDDGASGDTGIDAKSDDTPVVKFVNKVLIDAIRRGASDIHFEPYEEDYRVRFRIDGLLKGVAKAPN
KLNQRIAARLKVMSQLDIAEKRVPQDGRIKLNVSKTKQVDFRVSTLPTLFGEKVVLRILDGSAAKLGIDKLGYEADQQTL
FMEAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNNKRGMTFAAALRSFLRQDPD
VIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNKCKRPVQLPT
NALLAEGFTQEQIDAGLELYEPVGCDECTEGYKGRTGIYQVMPITEGISSIILAGGNALQIAEEAQRIGINDLRQSALLK
VAQGITGLAEINRVTKD

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=766344 O4444_RS10570 WP_010894965.1 2314187..2315920(+) (pilB) [Xylella fastidiosa subsp. pauca strain ALM4]
ATGAACACACCTCATTCAGCCAATCTTGTCGGCATCACCGGTATTGCCCGTCGCCTGGTCCAGGATGGCAGCATTGAGGA
AGCCACTGCACGCACTGCTCAAAATCAGGCAATGGCCGCAAAAGTGCCTTTACCACAGTGGTTGATCGATAAAAAACTAG
TAAGTACAGCAAAGTTGGCCGCAGCCAATGCACTTGAGTTCGGGATGCCGCTGCTGGATGTCTCCGCATTCGACCACAAT
CACAATGCAATGAGCTTGGTCGACGAAGAATTACTGCAAAAGCATCGGGTGTTGCCATTATTTAAACGCGGTAATCGACT
GTTCGTCGGGATCAGCAATCCAACCCAAACACAGGCCCTGGACGACATCAAATTCCACACCAATCTGGTCGTGGAACCGA
TTCTGGTCGACGAAGACCAGATACGGCGCACACTTGAACAATGGCAATCTAGGAGCGGCGCGCTCAGCTCCGCATTGGAG
GAAGAGGATAACGGCATTGAAGGGCTGGATATCGGCGGCGGAGACGACGACGGGGCCAGTGGCGATACCGGGATTGATGC
CAAGAGCGATGACACACCTGTCGTCAAATTCGTCAACAAAGTGCTGATTGATGCCATCCGACGCGGCGCATCAGACATCC
ACTTTGAACCATACGAAGAAGATTACCGCGTCCGTTTCCGGATTGATGGTCTGCTGAAGGGTGTGGCAAAAGCACCGAAC
AAGTTGAATCAGCGTATCGCCGCACGCTTGAAAGTCATGTCGCAACTGGATATCGCGGAAAAACGCGTCCCTCAAGACGG
ACGTATCAAGCTTAATGTGTCGAAAACCAAGCAAGTCGATTTCCGCGTCAGCACCTTGCCAACGCTGTTCGGCGAGAAAG
TGGTGCTGCGTATCTTGGATGGCAGCGCGGCAAAGCTGGGTATCGACAAACTCGGTTACGAAGCTGACCAACAAACGCTT
TTTATGGAAGCAATCCACAAACCCTATGGAATGGTATTGGTCACTGGTCCGACCGGTTCGGGCAAGACAGTGTCACTGTA
CACTGCACTAGGCATCCTCAATGACGAAACACGCAATATCTCCACAGCCGAAGATCCTGTAGAAATCCGGCTGCCGGGCG
TCAATCAAGTACAACAAAACAACAAACGCGGTATGACGTTTGCCGCAGCATTGCGTTCGTTTCTGCGCCAAGACCCGGAT
GTCATCATGGTCGGCGAAATCCGAGACCTGGAAACCGCTGAAATCGCCATCAAAGCCGCACAGACCGGCCACATGGTGCT
CTCCACACTACATACCAACGATGCACCGCAAACCATCGCACGCCTCATGAACATGGGGATTGCACCATACAACATCACTT
CATCCGTGACCTTAGTGATTGCACAGCGATTGGCACGGAGATTGTGCAATAAGTGCAAGCGCCCGGTTCAATTGCCGACA
AACGCATTACTGGCCGAAGGATTCACTCAAGAACAAATTGATGCGGGGCTGGAATTATACGAGCCTGTCGGTTGCGACGA
ATGCACCGAAGGCTACAAAGGCCGCACAGGTATTTATCAAGTGATGCCAATTACCGAAGGGATTTCCTCGATCATTTTAG
CCGGCGGTAATGCCTTACAAATCGCCGAAGAGGCGCAGCGCATTGGCATCAACGACCTACGCCAATCGGCGCTACTCAAA
GTCGCTCAGGGCATCACCGGGCTGGCTGAAATTAATCGAGTCACCAAAGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(200-467)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(48-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

57.143

98.267

0.562
  pilB Acinetobacter baumannii D1279779

56.261

98.267

0.553
  pilB Legionella pneumophila strain ERS1305867

51.761

98.44

0.51
  pilF Neisseria gonorrhoeae MS11

50.528

98.44

0.497
  pilB Vibrio cholerae strain A1552

49.54

94.107

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

47.5

97.054

0.461
  pilB Vibrio campbellii strain DS40M4

46.561

98.267

0.458
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.993

92.894

0.362
  pilF Thermus thermophilus HB27

40.077

89.948

0.36