Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OZ656_RS13765 Genome accession   NZ_CP114373
Coordinates   2947864..2949582 (+) Length   572 a.a.
NCBI ID   WP_379944639.1    Uniprot ID   -
Organism   Marinobacter sp. LM1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2942864..2954582
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OZ656_RS13730 (OZ656_13680) - 2943610..2943909 (-) 300 WP_023011844.1 CcdB family protein -
  OZ656_RS13735 (OZ656_13685) - 2943909..2944154 (-) 246 WP_081698882.1 type II toxin-antitoxin system CcdA family antitoxin -
  OZ656_RS13740 (OZ656_13690) - 2944421..2944726 (-) 306 WP_379944634.1 BrnA antitoxin family protein -
  OZ656_RS13745 - 2944710..2944910 (-) 201 WP_379944635.1 BrnT family toxin -
  OZ656_RS13750 (OZ656_13695) - 2944913..2945710 (+) 798 WP_379944636.1 transposase -
  OZ656_RS13755 (OZ656_13700) - 2946069..2946806 (-) 738 WP_379944637.1 hypothetical protein -
  OZ656_RS13760 (OZ656_13705) - 2946896..2947474 (-) 579 WP_379944638.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  OZ656_RS13765 (OZ656_13710) pilB 2947864..2949582 (+) 1719 WP_379944639.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OZ656_RS13770 (OZ656_13715) pilC 2949585..2950802 (+) 1218 WP_379944640.1 type II secretion system F family protein Machinery gene
  OZ656_RS13775 (OZ656_13720) pilD 2950886..2951761 (+) 876 WP_379944642.1 prepilin peptidase Machinery gene
  OZ656_RS13780 (OZ656_13725) coaE 2951762..2952379 (+) 618 WP_379944643.1 dephospho-CoA kinase -
  OZ656_RS13785 (OZ656_13730) - 2952468..2953457 (+) 990 WP_379944644.1 hypothetical protein -
  OZ656_RS13790 (OZ656_13735) tsaA 2953466..2954215 (+) 750 WP_114434283.1 tRNA (N6-threonylcarbamoyladenosine(37)-N6)-methyltransferase TrmO -

Sequence

Protein


Download         Length: 572 a.a.        Molecular weight: 62820.83 Da        Isoelectric Point: 4.9076

>NTDB_id=765706 OZ656_RS13765 WP_379944639.1 2947864..2949582(+) (pilB) [Marinobacter sp. LM1]
MASSNRNVTLTGLARRFVDDGLLDEETAKDAFIQASQNRIPLITYLTQNQLADSSKLAFSAAMEFGVSVLDLDSFLPEMM
PEKVVDEKLVRKHNALPLYKRGNRLFIAVSDPTNIQALDEIKFNTGLSTDAILVDDSKLRTTIDKYLESQDTTMGDLDDA
DLEGVETEGGEGEEDDGSVSATDIDDAPVVKYVNKMLLDAIRGGASDIHFEPYEKVYRVRYRTDGILKEVSRPSIKLAPK
ISARVKIMAQLDISERRVPQDGRIKMKLSKTKAIDFRVNTLPTLWGEKIVLRILDPSQAKLGIDVLGYEEDQKKLYMDAL
AQPQGMILVTGPTGSGKTVSLYTGLNILNTPGINISTAEDPAEINLEGINQVNVNNRVGLGFAEALRAFLRQDPDVIMVG
EIRDLETANIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIATSVSLIIAQRLGRRLCSSCKQPADVPKDVLLK
EGFTQEQIDTGFTLYRPKGCDKCNGGYKGRVGIYEVVKITDELASMIMEEASSIKIAKQAQAEGFRNLRQSALLKVIEGV
TSLEEANRVTKD

Nucleotide


Download         Length: 1719 bp        

>NTDB_id=765706 OZ656_RS13765 WP_379944639.1 2947864..2949582(+) (pilB) [Marinobacter sp. LM1]
ATGGCGAGCAGCAACCGAAACGTGACCTTGACAGGCTTGGCCCGTCGTTTTGTGGATGACGGCCTTTTAGATGAGGAAAC
TGCAAAAGACGCGTTCATCCAGGCATCCCAGAACCGAATTCCACTGATTACCTACCTGACTCAAAACCAACTTGCTGACA
GTTCCAAACTCGCATTTTCTGCAGCGATGGAATTCGGTGTTTCGGTGCTGGACCTGGACTCGTTTCTTCCGGAAATGATG
CCGGAGAAGGTCGTTGACGAAAAGCTGGTCCGCAAGCATAACGCGCTACCACTGTACAAACGAGGCAACCGGCTGTTCAT
TGCGGTTTCTGATCCCACTAACATTCAGGCGCTAGATGAGATCAAATTCAATACCGGCCTGAGCACAGATGCCATTCTGG
TTGATGACTCCAAGCTCCGTACGACTATTGACAAGTATCTAGAATCTCAAGACACCACCATGGGAGACTTGGACGATGCC
GATTTGGAGGGCGTCGAAACTGAGGGCGGTGAAGGTGAGGAGGATGATGGCTCCGTATCCGCGACGGATATCGATGATGC
ACCCGTCGTTAAGTATGTGAACAAGATGCTGTTGGATGCAATTCGGGGCGGGGCTTCAGATATTCACTTCGAGCCCTATG
AGAAAGTCTATCGTGTCCGCTACCGTACCGACGGCATATTGAAGGAGGTTTCACGGCCGTCCATTAAGCTGGCTCCGAAA
ATCTCTGCGCGTGTAAAGATCATGGCGCAGCTCGATATTTCTGAGCGCAGGGTACCACAGGACGGCCGGATCAAAATGAA
ACTGTCCAAAACCAAAGCCATTGATTTCCGGGTAAATACCCTGCCAACGCTTTGGGGCGAAAAGATTGTTTTGCGGATTC
TGGATCCAAGCCAGGCCAAGCTTGGCATCGACGTGCTGGGGTACGAGGAAGACCAGAAAAAGCTATATATGGATGCCCTG
GCACAGCCTCAAGGCATGATTTTGGTTACAGGACCAACCGGTTCCGGTAAAACGGTATCTCTTTACACCGGCCTAAATAT
TTTGAATACCCCAGGAATTAATATTTCAACGGCCGAAGACCCGGCGGAGATCAACCTGGAAGGCATTAACCAGGTAAACG
TGAATAACCGCGTGGGACTCGGCTTTGCGGAGGCGTTACGCGCATTCCTCCGACAGGATCCGGACGTCATCATGGTGGGT
GAGATCCGGGATCTGGAAACTGCCAACATCGCCATCAAAGCCGCGCAGACCGGGCACCTGGTTCTTTCCACCCTACACAC
GAACAGCGCAGCCGAGACTCTCACCCGCATGATGAACATGGGGGTGCCCGCTTTTAACATAGCCACATCAGTCAGCCTGA
TCATTGCCCAGCGCTTGGGCCGAAGACTCTGCAGCAGCTGCAAACAACCCGCCGATGTGCCTAAAGACGTTCTTTTAAAG
GAAGGGTTCACACAAGAACAAATTGATACAGGCTTCACGTTGTACCGCCCCAAGGGCTGTGATAAATGCAATGGTGGATA
TAAAGGCCGCGTTGGTATTTACGAGGTGGTCAAAATTACCGATGAGCTGGCAAGCATGATTATGGAAGAAGCCAGCTCCA
TCAAAATTGCAAAGCAGGCTCAGGCGGAAGGCTTCCGCAACCTCAGGCAATCGGCGCTTCTCAAGGTTATAGAGGGAGTG
ACCAGTCTTGAAGAAGCCAACCGAGTCACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(195-462)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-152)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.719

100

0.568
  pilB Acinetobacter baylyi ADP1

56.56

98.601

0.558
  pilB Legionella pneumophila strain ERS1305867

53.553

100

0.54
  pilB Vibrio parahaemolyticus RIMD 2210633

51.712

97.028

0.502
  pilB Vibrio campbellii strain DS40M4

49.912

98.776

0.493
  pilB Vibrio cholerae strain A1552

53.95

90.734

0.49
  pilF Neisseria gonorrhoeae MS11

48.325

99.126

0.479
  pilB/pilB1 Synechocystis sp. PCC 6803

37.296

100

0.4
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.417

92.133

0.372