Detailed information NTG0764797

Bioinformatically predicted

Overview

• Name: comEA
• Type: Machinery gene
• Locus tag: M1L51_RS12125
• Genome accession: NZ_CP114180
• Coordinates: 2537098..2537712 (-)
• Length: 204 a.a.
• NCBI ID: WP_053573180.1
• Uniprot ID: -
• Organism: Bacillus velezensis strain DMW1
• Function: dsDNA binding to the cell surface (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2532098..2542712

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
M1L51_RS12095	rpsT	2532398..2532664 (+)	267	WP_003152876.1	30S ribosomal protein S20	-
M1L51_RS12100	holA	2532681..2533722 (-)	1042	Protein_2337	DNA polymerase III subunit delta	-
M1L51_RS12105	-	2533762..2533916 (-)	155	Protein_2338	hypothetical protein	-
M1L51_RS12110	-	2533957..2534091 (+)	135	WP_003152870.1	YqzM family protein	-
M1L51_RS12115	comEC	2534110..2536461 (-)	2352	WP_053573181.1	DNA internalization-related competence protein ComEC/Rec2	Machinery gene
M1L51_RS12120	-	2536462..2537031 (-)	570	WP_003152868.1	ComE operon protein 2	-
M1L51_RS12125	comEA	2537098..2537712 (-)	615	WP_053573180.1	helix-hairpin-helix domain-containing protein	Machinery gene
M1L51_RS12130	comER	2537771..2538592 (+)	822	WP_015417855.1	late competence protein ComER	-
M1L51_RS12135	-	2538661..2539398 (-)	738	WP_015417856.1	class I SAM-dependent DNA methyltransferase	-
M1L51_RS12140	rsfS	2539395..2539751 (-)	357	WP_007408260.1	ribosome silencing factor	-
M1L51_RS12145	yqeK	2539769..2540329 (-)	561	WP_014418422.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) YqeK	-
M1L51_RS12150	-	2540319..2540888 (-)	570	WP_007408258.1	nicotinate-nucleotide adenylyltransferase	-
M1L51_RS12155	yhbY	2540899..2541189 (-)	291	WP_003152858.1	ribosome assembly RNA-binding protein YhbY	-
M1L51_RS12160	aroE	2541183..2542025 (-)	843	WP_039254404.1	shikimate dehydrogenase	-

Sequence

Protein

• Length: 204 a.a.
• Molecular weight: 22183.34 Da
• Isoelectric Point: 6.4888

>NTDB_id=764797 M1L51_RS12125 WP_053573180.1 2537098..2537712(-) (comEA) [Bacillus velezensis strain DMW1]
MTKAMMTERLRRNLKILGACLAAGAVFICIWLFRSEKEEPVKQQSVLGQTASASVADKKDTAADKIMIDVKGAVRNPGVY
EMKAGDRVTQAIEKAGGLKKKADELNVNLAEQLQDGTMVYIPSEGEEENRPKTAAGEKENAAVNINTASLDELQAISGVG
QKKAEAIIAYREENGRFQTAEDLMNVSGFGEKSFERIKTSITVK

Nucleotide

• Length: 615 bp

>NTDB_id=764797 M1L51_RS12125 WP_053573180.1 2537098..2537712(-) (comEA) [Bacillus velezensis strain DMW1]
ATGACGAAAGCGATGATGACGGAGCGGCTGCGGCGGAACCTGAAAATTCTGGGCGCTTGTCTTGCGGCAGGGGCGGTTTT
TATCTGCATTTGGCTGTTTCGTTCTGAAAAGGAAGAGCCGGTCAAACAGCAGTCCGTGCTCGGCCAAACAGCCTCAGCAT
CCGTTGCGGACAAAAAAGACACCGCAGCCGATAAGATTATGATTGATGTGAAAGGTGCGGTCCGAAATCCCGGCGTGTAT
GAAATGAAAGCGGGAGACAGAGTGACGCAAGCCATTGAAAAAGCCGGCGGCCTGAAGAAAAAGGCGGATGAATTGAATGT
TAATTTAGCAGAGCAGCTGCAGGACGGGACGATGGTTTATATTCCGAGTGAAGGCGAAGAGGAGAATCGCCCGAAAACAG
CGGCGGGTGAAAAGGAGAATGCAGCTGTCAATATCAATACGGCATCTCTGGACGAACTTCAGGCGATTTCCGGGGTGGGG
CAGAAAAAAGCGGAAGCGATTATCGCCTACCGAGAAGAAAACGGACGGTTTCAGACTGCTGAAGATTTGATGAATGTATC
AGGCTTTGGAGAAAAGTCATTTGAGCGGATAAAAACTTCCATTACGGTAAAGTGA

Domains

Predicted by InterproScan.

• SLBB domain (SLBB) (68-122)

• Helix-hairpin-helix motif (HHH_3) (141-202)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comEA	Bacillus subtilis subsp. subtilis str. 168	58.854	94.118	0.554
comEA	Latilactobacillus sakei subsp. sakei 23K	32.328	100	0.368
comEA/celA/cilE	Streptococcus mitis NCTC 12261	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae Rx1	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae D39	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae R6	35.545	100	0.368
comEA/celA/cilE	Streptococcus pneumoniae TIGR4	35.545	100	0.368
comEA/celA/cilE	Streptococcus mitis SK321	35.071	100	0.363