Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OZ429_RS14255 Genome accession   NZ_CP114134
Coordinates   3030756..3032492 (-) Length   578 a.a.
NCBI ID   WP_269122565.1    Uniprot ID   -
Organism   Xanthomonas fragariae strain YLX21     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3025756..3037492
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OZ429_RS14230 (OZ429_14230) - 3026569..3028182 (+) 1614 WP_269122562.1 HAMP domain-containing sensor histidine kinase -
  OZ429_RS14235 (OZ429_14235) pilR 3028411..3029859 (+) 1449 WP_330873184.1 sigma-54 dependent transcriptional regulator Regulator
  OZ429_RS14240 (OZ429_14240) - 3029933..3030115 (+) 183 WP_269122564.1 YhfG family protein -
  OZ429_RS14245 (OZ429_14245) - 3030242..3030379 (+) 138 Protein_2681 cell filamentation protein Fic -
  OZ429_RS19650 - 3030507..3030665 (+) 159 WP_330873185.1 hypothetical protein -
  OZ429_RS14255 (OZ429_14255) pilB 3030756..3032492 (-) 1737 WP_269122565.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OZ429_RS14260 (OZ429_14260) pilA/pilAI 3032609..3033016 (-) 408 WP_208589544.1 pilin Machinery gene
  OZ429_RS14265 (OZ429_14265) pilA 3033091..3033501 (-) 411 WP_208589542.1 pilin Machinery gene
  OZ429_RS14270 (OZ429_14270) pilC 3033869..3035125 (+) 1257 WP_208591561.1 type II secretion system F family protein Machinery gene
  OZ429_RS14275 (OZ429_14275) - 3035133..3035996 (+) 864 WP_208589534.1 A24 family peptidase -
  OZ429_RS14280 (OZ429_14280) coaE 3036010..3036642 (+) 633 WP_208589532.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 578 a.a.        Molecular weight: 62561.78 Da        Isoelectric Point: 5.4429

>NTDB_id=764505 OZ429_RS14255 WP_269122565.1 3030756..3032492(-) (pilB) [Xanthomonas fragariae strain YLX21]
MSAVTSANLVGITGIARRLVQDGALEETVARVAMDQAAAAKIPLPQWFSEKKLVSASQLAAANAVEFGMPLMDVSVFDAS
QNAVKLVSEELLQKYQVLPLFKRGNRLFVGVSNPTQTRALDDIKFHTNLVVEPILVDEDQIRRTLEQWQASNAALGSSLG
DDDEGMGDLDVSAGDEDMGAGGDSGVDAKGDDTPVVKFVNKMLVDAIRRGASDIHFEPYEDDYRVRLRIDGLLKNVAKAP
VKLSQRIAARLKVMSQLDIAEKRVPQDGRIKLNLSKTKQIDFRVSTLPTLFGEKVVLRILDASAAKLGIEKLGYEADQQT
LFLDAIHKPYGMVLVTGPTGSGKTVSLYTALGILNDETRNISTAEDPVEIRLPGVNQVQQNVKRGMTFAAALRSFLRQDP
DIIMVGEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPQTIARLMNMGIAPYNITSSVTLVIAQRLARRLCNNCKRKSTLP
EHALLAEGFTAEQLAAGIELYEAVGCDECTAGYKGRTGIYQVMPMTEEIGAIVLEGGNAMQIAEAAQSIGIKDLRQSALI
KAAHGVTSLAEINRVTKD

Nucleotide


Download         Length: 1737 bp        

>NTDB_id=764505 OZ429_RS14255 WP_269122565.1 3030756..3032492(-) (pilB) [Xanthomonas fragariae strain YLX21]
ATGAGCGCGGTCACTTCTGCTAATTTGGTCGGCATTACCGGCATAGCCAGACGCCTAGTTCAGGACGGCGCCTTGGAAGA
AACGGTGGCGCGGGTTGCAATGGACCAGGCTGCGGCAGCTAAGATTCCTCTGCCACAATGGTTTTCAGAGAAAAAGTTGG
TTTCGGCTTCACAACTGGCTGCTGCCAACGCAGTCGAGTTCGGCATGCCGCTGATGGACGTGTCGGTGTTCGACGCTAGC
CAGAATGCGGTCAAGCTGGTCAGTGAGGAGTTGCTCCAGAAGTACCAGGTGCTGCCGCTGTTCAAGCGCGGCAATCGGTT
ATTCGTGGGGGTGAGCAACCCGACCCAGACCCGGGCGCTGGACGACATCAAGTTCCACACCAATCTGGTGGTGGAGCCGA
TCTTGGTCGACGAGGACCAGATCCGTCGCACCCTGGAGCAGTGGCAGGCCAGCAATGCGGCGCTTGGCTCGTCGCTCGGT
GACGACGATGAGGGGATGGGGGACCTGGACGTGTCGGCCGGGGACGAGGACATGGGCGCCGGCGGGGATTCCGGGGTCGA
TGCCAAGGGCGACGACACGCCGGTGGTGAAGTTCGTCAACAAGATGCTGGTGGATGCGATCCGGCGGGGAGCCTCCGACA
TCCATTTCGAGCCGTATGAAGACGATTACCGGGTGCGGTTGCGGATCGATGGGCTGCTGAAGAACGTGGCCAAGGCGCCG
GTGAAACTGAGCCAGCGCATCGCGGCGCGCTTGAAGGTGATGTCGCAGCTGGACATCGCCGAGAAACGGGTGCCGCAGGA
CGGGCGCATCAAGCTCAATCTGTCCAAGACCAAGCAGATCGACTTCCGTGTCAGCACCTTGCCGACCTTGTTCGGCGAGA
AGGTGGTGCTGCGTATTTTGGACGCTAGCGCGGCCAAGCTGGGCATCGAGAAGTTGGGCTATGAGGCGGATCAGCAGACG
CTGTTCCTGGATGCGATCCACAAGCCGTACGGCATGGTGCTGGTGACCGGGCCGACCGGTTCGGGCAAGACGGTGTCGTT
GTATACGGCGCTGGGCATCCTCAACGATGAGACGCGCAATATCTCCACGGCCGAGGATCCGGTGGAAATCCGGTTACCGG
GCGTGAACCAGGTGCAGCAGAACGTCAAGCGGGGCATGACGTTTGCGGCGGCGTTGCGGTCGTTTCTGCGCCAGGATCCG
GACATCATCATGGTCGGCGAAATCCGCGACCTGGAAACGGCCGAGATTGCGATCAAGGCGGCGCAGACCGGCCACATGGT
GTTGTCGACGTTGCACACCAACGATGCGCCGCAGACCATTGCGCGTCTGATGAACATGGGCATTGCGCCGTACAACATTA
CCTCATCGGTGACGTTGGTCATTGCACAGCGTCTGGCGCGGCGGTTGTGCAACAACTGCAAGCGCAAGTCGACGTTGCCC
GAGCACGCATTGCTTGCGGAAGGATTCACCGCGGAGCAGTTGGCTGCAGGTATCGAACTATATGAGGCGGTGGGCTGCGA
CGAGTGCACCGCAGGCTATAAGGGCCGTACCGGTATTTACCAGGTGATGCCGATGACCGAAGAGATCGGCGCGATCGTGC
TGGAAGGCGGCAATGCAATGCAGATCGCCGAGGCGGCGCAGAGTATCGGCATCAAAGATCTGCGGCAATCGGCGCTGATC
AAGGCGGCGCATGGGGTGACCAGCCTGGCCGAGATCAATCGGGTGACGAAGGATTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.44

100

0.555
  pilB Acinetobacter baylyi ADP1

54.922

100

0.55
  pilB Legionella pneumophila strain ERS1305867

53.076

98.443

0.522
  pilB Vibrio cholerae strain A1552

48.7

99.827

0.486
  pilF Neisseria gonorrhoeae MS11

48.768

98.27

0.479
  pilB Vibrio parahaemolyticus RIMD 2210633

46.643

97.924

0.457
  pilB Vibrio campbellii strain DS40M4

46.221

98.443

0.455