Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   OZ675_RS14210 Genome accession   NZ_CP113978
Coordinates   3065294..3065803 (-) Length   169 a.a.
NCBI ID   WP_020831292.1    Uniprot ID   A0A454TT91
Organism   Ralstonia pseudosolanacearum strain RS26     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3060294..3070803
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OZ675_RS14190 (OZ675_14200) - 3060396..3060983 (+) 588 WP_020831295.1 DUF2946 family protein -
  OZ675_RS14195 (OZ675_14205) - 3060998..3062686 (-) 1689 WP_071623449.1 M48 family metallopeptidase -
  OZ675_RS14200 (OZ675_14210) moaC 3062780..3063256 (+) 477 WP_011000519.1 cyclic pyranopterin monophosphate synthase MoaC -
  OZ675_RS14205 (OZ675_14215) - 3063484..3065217 (-) 1734 WP_089190455.1 Wzy polymerase domain-containing protein -
  OZ675_RS14210 (OZ675_14220) pilA 3065294..3065803 (-) 510 WP_020831292.1 pilin Machinery gene
  OZ675_RS14215 (OZ675_14225) pilA 3066070..3066591 (-) 522 WP_028852632.1 pilin Machinery gene
  OZ675_RS14220 (OZ675_14230) - 3066785..3067483 (-) 699 WP_089190456.1 TerC family protein -
  OZ675_RS14225 (OZ675_14235) sucD 3067633..3068514 (-) 882 WP_011000514.1 succinate--CoA ligase subunit alpha -
  OZ675_RS14230 (OZ675_14240) sucC 3068599..3069765 (-) 1167 WP_011000513.1 ADP-forming succinate--CoA ligase subunit beta -
  OZ675_RS14235 (OZ675_14245) - 3069832..3070458 (-) 627 WP_029240215.1 DUF2889 domain-containing protein -

Sequence

Protein


Download         Length: 169 a.a.        Molecular weight: 17122.83 Da        Isoelectric Point: 9.9491

>NTDB_id=763965 OZ675_RS14210 WP_020831292.1 3065294..3065803(-) (pilA) [Ralstonia pseudosolanacearum strain RS26]
MKSMRHLNKRVQKGFTLIELMIVVAIVGILAAIAIPAYQDYTVRARVTEGLSLAAQAKALVSENAANAQTDLSVGSSVFT
PTKNVAGLAIDGTTTGVPGQITITYTQAAGGGTLALVPTAAGAKLPTSSAPSGPIMWTCYAAGKAQAASSVAPNATMSLA
AKYAPAECR

Nucleotide


Download         Length: 510 bp        

>NTDB_id=763965 OZ675_RS14210 WP_020831292.1 3065294..3065803(-) (pilA) [Ralstonia pseudosolanacearum strain RS26]
ATGAAGTCGATGCGTCATCTCAACAAGCGTGTCCAGAAGGGTTTCACGCTGATCGAACTGATGATCGTGGTCGCGATCGT
CGGTATTCTGGCTGCGATTGCCATTCCGGCCTATCAGGACTACACGGTTCGTGCACGCGTGACGGAAGGTCTGTCGCTGG
CTGCGCAAGCCAAGGCGCTGGTGTCGGAAAACGCTGCCAATGCACAGACTGACCTGTCGGTGGGTTCGTCGGTGTTCACG
CCCACCAAGAACGTTGCAGGCCTTGCAATTGACGGTACTACGACTGGTGTTCCGGGCCAGATCACCATCACCTATACGCA
GGCTGCCGGCGGCGGCACGCTGGCTCTGGTCCCGACCGCGGCAGGCGCTAAGCTGCCGACGAGTTCGGCTCCGTCGGGTC
CGATCATGTGGACCTGCTACGCTGCGGGTAAGGCACAGGCTGCCAGCAGCGTTGCTCCGAACGCTACGATGTCGCTGGCA
GCGAAGTATGCTCCGGCAGAATGCCGCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(43-169)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-34)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A454TT91

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

91.124

100

0.911
  pilA2 Legionella pneumophila str. Paris

51.235

95.858

0.491
  pilA2 Legionella pneumophila strain ERS1305867

50.617

95.858

0.485
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

38.462

100

0.444
  comP Acinetobacter baylyi ADP1

43.023

100

0.438
  pilA/pilA1 Eikenella corrodens VA1

38.506

100

0.396