Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   OZ675_RS14215 Genome accession   NZ_CP113978
Coordinates   3066070..3066591 (-) Length   173 a.a.
NCBI ID   WP_028852632.1    Uniprot ID   A0A454TT80
Organism   Ralstonia pseudosolanacearum strain RS26     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3061070..3071591
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OZ675_RS14200 (OZ675_14210) moaC 3062780..3063256 (+) 477 WP_011000519.1 cyclic pyranopterin monophosphate synthase MoaC -
  OZ675_RS14205 (OZ675_14215) - 3063484..3065217 (-) 1734 WP_089190455.1 Wzy polymerase domain-containing protein -
  OZ675_RS14210 (OZ675_14220) pilA 3065294..3065803 (-) 510 WP_020831292.1 pilin Machinery gene
  OZ675_RS14215 (OZ675_14225) pilA 3066070..3066591 (-) 522 WP_028852632.1 pilin Machinery gene
  OZ675_RS14220 (OZ675_14230) - 3066785..3067483 (-) 699 WP_089190456.1 TerC family protein -
  OZ675_RS14225 (OZ675_14235) sucD 3067633..3068514 (-) 882 WP_011000514.1 succinate--CoA ligase subunit alpha -
  OZ675_RS14230 (OZ675_14240) sucC 3068599..3069765 (-) 1167 WP_011000513.1 ADP-forming succinate--CoA ligase subunit beta -
  OZ675_RS14235 (OZ675_14245) - 3069832..3070458 (-) 627 WP_029240215.1 DUF2889 domain-containing protein -
  OZ675_RS14240 (OZ675_14250) recX 3070733..3071212 (-) 480 WP_020831287.1 recombination regulator RecX -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17776.47 Da        Isoelectric Point: 10.2418

>NTDB_id=763966 OZ675_RS14215 WP_028852632.1 3066070..3066591(-) (pilA) [Ralstonia pseudosolanacearum strain RS26]
MASKHSRNRRRGQRGFTLIELMIVVAIIGILAALAIPAYQDYTIRSRVIEGLALAGQAKALVVENAANAQSSLSLGSASL
PASKNVSALNIDSSTGEIAVVYASAVTGAGANTLVLTPYSGSSSVFGNLQAGSTPPAMVLWVCAAAGKSMPVNTVTQRTT
ATLPPKYAPSECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=763966 OZ675_RS14215 WP_028852632.1 3066070..3066591(-) (pilA) [Ralstonia pseudosolanacearum strain RS26]
ATGGCATCGAAGCACAGCAGGAACAGGCGGCGCGGCCAGCGCGGCTTTACATTGATCGAGTTGATGATCGTGGTGGCGAT
CATCGGGATTTTGGCGGCGCTGGCGATTCCGGCGTATCAGGACTACACGATCCGGTCGCGTGTGATCGAGGGGCTGGCAC
TGGCAGGGCAAGCCAAGGCGCTGGTGGTTGAAAATGCCGCCAACGCACAGAGTAGCCTGTCACTCGGTTCCGCATCGTTG
CCGGCCAGCAAGAACGTCAGTGCTCTGAATATTGATTCGTCCACCGGCGAAATCGCGGTGGTCTATGCATCCGCTGTTAC
GGGAGCGGGGGCGAATACGTTGGTATTGACCCCATACTCCGGCAGTTCGTCAGTATTTGGCAATTTGCAGGCAGGTTCAA
CGCCACCGGCAATGGTGCTGTGGGTGTGTGCCGCCGCCGGCAAGTCGATGCCGGTCAATACCGTTACCCAGAGGACGACG
GCTACGTTGCCACCGAAATACGCGCCCTCGGAGTGCCGTTGA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(45-173)

Prokaryotic N-terminal methylation motif (N_methyl)

(10-35)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A454TT80

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

51.705

100

0.526
  pilA2 Legionella pneumophila str. Paris

44.848

95.376

0.428
  pilA2 Legionella pneumophila strain ERS1305867

44.242

95.376

0.422
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.263

100

0.387
  comP Acinetobacter baylyi ADP1

40.741

93.642

0.382