Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OYW20_RS22350 Genome accession   NZ_CP113949
Coordinates   4955964..4957658 (+) Length   564 a.a.
NCBI ID   WP_268798070.1    Uniprot ID   -
Organism   Pseudomonas huanghezhanensis strain BSw22131     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 4950964..4962658
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OYW20_RS22330 - 4951460..4953706 (-) 2247 WP_268798067.1 DUF1631 domain-containing protein -
  OYW20_RS22335 nadC 4954078..4954926 (+) 849 WP_268798068.1 carboxylating nicotinate-nucleotide diphosphorylase -
  OYW20_RS22345 - 4955304..4955738 (-) 435 WP_268798069.1 pilin -
  OYW20_RS22350 pilB 4955964..4957658 (+) 1695 WP_268798070.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OYW20_RS22355 pilC 4957661..4958878 (+) 1218 WP_268798071.1 type II secretion system F family protein Machinery gene
  OYW20_RS22360 pilD 4958882..4959754 (+) 873 WP_268798072.1 A24 family peptidase Machinery gene
  OYW20_RS22365 coaE 4959751..4960374 (+) 624 WP_268798073.1 dephospho-CoA kinase -
  OYW20_RS22370 yacG 4960371..4960574 (+) 204 WP_268798074.1 DNA gyrase inhibitor YacG -
  OYW20_RS22375 - 4960628..4961089 (-) 462 WP_268798075.1 hypothetical protein -
  OYW20_RS22380 - 4961233..4961862 (+) 630 WP_268798076.1 DUF1780 domain-containing protein -
  OYW20_RS22385 - 4961859..4962398 (+) 540 WP_268798077.1 MOSC domain-containing protein -
  OYW20_RS22390 - 4962440..4962553 (+) 114 WP_408005529.1 hypothetical protein -

Sequence

Protein


Download         Length: 564 a.a.        Molecular weight: 62333.74 Da        Isoelectric Point: 6.0119

>NTDB_id=763765 OYW20_RS22350 WP_268798070.1 4955964..4957658(+) (pilB) [Pseudomonas huanghezhanensis strain BSw22131]
MTDVVLTGLAKQLVVAELLNEKAAQQAYQQARRDKVSLVHHLVQSKLVKSLTLAEVASDQFGIPFLDLNMLDKESQPKGL
VSEKLVRQHCALPLWRRGNKLFVGISDPTNHQAITDIQFSTGLTTEAILVEDDKLTIAIDKFFDSNGGMGDLADVDLGLE
IETVDESKESSIGGQDADDAPVVRFVNKMLMDAIRLGSSDLHFEPYEKMFRVRLRTDGILHEVARPPIHLAGRIAARLKV
MASLDISERRKPQDGRIKLRISKNRAIDFRVNTLPTLWGEKIVMRILDPTTAQMGIDALGYEPEQKALYLEALRQPQGMI
LVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINLEGINQVNVNPRQGMDFSQALRAFLRQDPDVIMVGEIRDLET
AEIAIKASQTGHMVLSTLHTNSAAETLTRLHHMGVAAFNIATAINLIIAQRLARKLCPYCKKEVDIPRETLIAEGFPEAK
IGSFKLYSPVGCDQCNGGYKGRVGIYEVVKSTPALERIIMEEGNSIEISEQMRKDGFNDLRTSGLMKAMQGVTSLEEINR
VTKD

Nucleotide


Download         Length: 1695 bp        

>NTDB_id=763765 OYW20_RS22350 WP_268798070.1 4955964..4957658(+) (pilB) [Pseudomonas huanghezhanensis strain BSw22131]
ATGACTGATGTCGTCTTAACCGGTTTGGCGAAGCAACTCGTTGTTGCCGAACTGCTCAATGAAAAAGCTGCGCAGCAGGC
TTACCAACAGGCTCGCCGTGACAAGGTGTCGCTGGTTCATCACCTGGTACAAAGCAAGCTGGTCAAAAGCCTGACGCTGG
CTGAAGTGGCATCTGACCAGTTCGGCATTCCTTTTCTTGATCTGAATATGCTGGACAAGGAAAGCCAGCCCAAAGGGTTG
GTCAGCGAAAAGCTGGTTCGTCAGCATTGCGCGCTCCCTCTGTGGCGGCGTGGCAATAAGTTGTTCGTGGGTATTTCGGA
CCCGACAAATCACCAAGCCATTACCGACATCCAGTTCAGCACCGGCCTGACAACCGAAGCAATTCTCGTCGAAGACGACA
AGCTCACCATCGCCATCGATAAATTTTTTGACAGCAATGGCGGAATGGGCGATCTGGCCGACGTCGATCTCGGCCTTGAA
ATCGAAACTGTCGACGAGAGCAAAGAGAGCTCGATTGGCGGTCAGGATGCGGACGACGCGCCGGTCGTGCGTTTCGTTAA
CAAGATGCTGATGGACGCCATCCGCCTGGGCTCCTCCGACCTTCATTTCGAACCTTATGAAAAGATGTTCCGCGTGCGTC
TGCGTACCGACGGGATCTTGCACGAGGTGGCGAGGCCGCCCATTCATCTGGCCGGTCGAATTGCTGCACGCTTGAAGGTG
ATGGCGAGTCTGGACATTTCAGAACGACGCAAACCGCAAGACGGCCGCATCAAGCTGCGCATCTCAAAAAACCGTGCGAT
CGATTTCCGGGTTAACACGCTGCCGACACTGTGGGGCGAGAAAATCGTGATGCGGATTCTCGACCCGACCACGGCGCAGA
TGGGCATCGATGCGCTGGGTTATGAGCCGGAGCAAAAGGCACTTTATCTGGAAGCCTTGCGCCAGCCGCAGGGCATGATC
CTGGTGACAGGCCCAACCGGTTCCGGCAAGACCGTTTCGCTTTATACCGGCCTCAACATTCTCAACACGGTAGACATCAA
CATCTCCACCGCCGAAGACCCGGTTGAAATCAACCTGGAAGGCATCAATCAGGTCAACGTCAACCCGCGTCAGGGCATGG
ACTTCTCGCAGGCGCTGCGCGCGTTTCTGCGGCAAGACCCGGACGTGATCATGGTCGGGGAGATTCGTGACCTGGAGACG
GCCGAGATCGCGATCAAGGCCTCACAAACCGGTCACATGGTGCTGTCGACGCTGCACACCAACAGTGCTGCAGAAACCCT
GACCCGCCTGCACCACATGGGCGTCGCAGCGTTCAACATCGCGACCGCGATCAACCTGATCATCGCCCAGCGGCTGGCAC
GCAAATTGTGCCCCTACTGCAAGAAGGAAGTGGATATCCCCCGCGAGACTTTGATTGCGGAAGGTTTCCCCGAGGCAAAA
ATCGGCAGTTTTAAGCTTTACTCCCCAGTGGGCTGCGACCAGTGCAACGGCGGCTACAAAGGCCGCGTCGGGATTTACGA
AGTGGTTAAAAGCACGCCGGCGCTGGAACGCATCATTATGGAAGAAGGCAACTCCATCGAGATTTCCGAGCAGATGCGCA
AGGACGGTTTCAACGACCTGCGCACCTCCGGCCTGATGAAGGCCATGCAGGGCGTGACCAGCCTTGAGGAAATCAACCGG
GTGACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

57.624

100

0.576
  pilB Acinetobacter baylyi ADP1

56.762

99.645

0.566
  pilB Legionella pneumophila strain ERS1305867

54.417

100

0.546
  pilB Vibrio cholerae strain A1552

50.532

100

0.505
  pilB Vibrio campbellii strain DS40M4

49.736

100

0.502
  pilB Vibrio parahaemolyticus RIMD 2210633

50.364

97.518

0.491
  pilF Neisseria gonorrhoeae MS11

49.288

99.645

0.491
  pilF Thermus thermophilus HB27

38.129

98.582

0.376
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.313

92.908

0.365