Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OXH62_RS12765 Genome accession   NZ_CP113803
Coordinates   2779673..2781373 (+) Length   566 a.a.
NCBI ID   WP_375747900.1    Uniprot ID   -
Organism   Pseudomonas chlororaphis strain YJY915     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2774673..2786373
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OXH62_RS12745 (OXH62_12685) - 2774947..2777898 (+) 2952 WP_375747898.1 DUF748 domain-containing protein -
  OXH62_RS12750 (OXH62_12690) - 2777898..2778194 (+) 297 WP_375747899.1 DUF2845 domain-containing protein -
  OXH62_RS12755 (OXH62_12695) - 2778362..2778718 (-) 357 WP_085608680.1 BON domain-containing protein -
  OXH62_RS12760 (OXH62_12700) - 2779030..2779446 (-) 417 WP_011335978.1 pilin -
  OXH62_RS12765 (OXH62_12705) pilB 2779673..2781373 (+) 1701 WP_375747900.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OXH62_RS12770 (OXH62_12710) pilC 2781376..2782593 (+) 1218 WP_375747901.1 type II secretion system F family protein Machinery gene
  OXH62_RS12775 (OXH62_12715) pilD 2782595..2783467 (+) 873 WP_127799972.1 A24 family peptidase Machinery gene
  OXH62_RS12780 (OXH62_12720) coaE 2783464..2784087 (+) 624 WP_007959586.1 dephospho-CoA kinase -
  OXH62_RS12785 (OXH62_12725) yacG 2784084..2784284 (+) 201 WP_007959585.1 DNA gyrase inhibitor YacG -
  OXH62_RS12790 (OXH62_12730) - 2784291..2784506 (-) 216 WP_007959584.1 hypothetical protein -
  OXH62_RS12795 (OXH62_12735) - 2784572..2785261 (-) 690 WP_115079246.1 energy-coupling factor ABC transporter permease -
  OXH62_RS12800 (OXH62_12740) - 2785395..2786021 (+) 627 WP_007959580.1 DUF1780 domain-containing protein -

Sequence

Protein


Download         Length: 566 a.a.        Molecular weight: 62762.74 Da        Isoelectric Point: 5.9461

>NTDB_id=763138 OXH62_RS12765 WP_375747900.1 2779673..2781373(+) (pilB) [Pseudomonas chlororaphis strain YJY915]
MNDIALSGLAKQLVQAELLTEKSAQQAWQQAQRNRLSLVSYLVQNKLVKSWQVAEIASEHFGMAFLDLNCLDKETQPKGL
VSEKLVRQHHALPLWRRGNKLFVGISDPSNHQAINDIQFSTGLSTEAILVEDDKLTDAIEKFFDTHATGLEEMADVDLDG
LDIESADDGKQDTLGGIDADDAPVVRFVHKMLLDAIKSGSSDLHFEPYEKNYRVRVRTDGILREVAKPPIQLAGRIAARL
KVMASLDISERRKPQDGRIKMRLSKSKSIDFRVNTLPTLWGEKVVMRILDPSSAQIGIDALGYEPEQKDLYMAALKQPQG
MILVTGPTGSGKTVSLYTGLNILNTVDINISTAEDPVEINMEGINQVNVNPRQGLDFAQALRSFLRQDPDVIMVGEIRDL
ETAEIAIKAAQTGHLVLSTLHTNSAAETLTRLHNMGIPGFNIATSVSLIIAQRLARKLCSHCKKPIEIPRETLIKEGFPE
ERIGHFTIYEPSGCDHCNGGYKGRVGIYEVVKNTPDLQRLIMAEGNSLEIDTQMRRDGFNDLRTSGLIKAMQGITSLEEI
NRVTKD

Nucleotide


Download         Length: 1701 bp        

>NTDB_id=763138 OXH62_RS12765 WP_375747900.1 2779673..2781373(+) (pilB) [Pseudomonas chlororaphis strain YJY915]
ATGAATGACATCGCTCTGAGCGGTCTGGCCAAACAACTGGTCCAGGCCGAATTGCTCACGGAAAAGAGTGCCCAGCAAGC
CTGGCAGCAGGCGCAGCGCAATCGCCTGTCGCTGGTCAGTTACCTGGTGCAGAACAAACTGGTGAAGAGCTGGCAGGTCG
CCGAAATAGCCTCGGAGCATTTCGGCATGGCATTTCTCGACCTCAACTGTCTCGACAAGGAAACCCAACCAAAGGGCCTG
GTCAGTGAAAAACTGGTGCGTCAGCACCACGCCCTGCCCCTCTGGCGGCGCGGCAACAAACTGTTCGTGGGGATCTCCGA
CCCGAGTAATCATCAGGCGATCAATGACATTCAGTTCAGCACCGGGCTGAGCACCGAAGCCATTCTGGTCGAGGACGACA
AACTCACCGACGCCATCGAAAAATTCTTCGACACCCACGCCACCGGCCTGGAGGAGATGGCCGATGTCGACCTCGACGGT
CTGGACATCGAGTCGGCCGATGACGGCAAGCAGGACACGCTCGGCGGAATAGACGCCGACGATGCCCCGGTGGTGCGCTT
CGTGCACAAGATGCTGCTGGACGCGATCAAGAGCGGCTCGTCCGACCTGCACTTCGAACCTTACGAAAAGAATTACCGGG
TGCGGGTGCGTACCGACGGCATCCTGCGCGAAGTGGCCAAGCCGCCGATTCAGCTCGCCGGGCGCATTGCCGCGCGCCTG
AAGGTCATGGCCAGCCTCGACATTTCGGAACGGCGCAAACCCCAGGACGGGCGGATCAAGATGCGTCTGTCGAAGAGCAA
GTCGATCGATTTCCGGGTCAACACCCTGCCCACCCTCTGGGGCGAGAAAGTGGTGATGCGGATCCTCGACCCGTCCAGCG
CCCAGATCGGCATCGATGCCCTCGGCTACGAGCCGGAGCAGAAAGACCTGTACATGGCCGCCCTCAAGCAGCCCCAAGGC
ATGATTCTGGTAACCGGCCCCACCGGTTCGGGCAAAACCGTGTCGCTGTACACCGGGCTGAACATCCTCAATACCGTCGA
CATCAACATCTCCACCGCCGAAGACCCGGTGGAGATCAACATGGAAGGCATCAACCAGGTCAACGTCAATCCACGTCAGG
GGTTGGACTTTGCCCAGGCCCTGCGCTCGTTCCTGCGTCAGGACCCGGACGTGATCATGGTCGGCGAGATCCGCGACCTC
GAAACCGCCGAAATCGCGATCAAGGCCGCCCAGACCGGGCACCTCGTGTTGTCTACCCTGCACACCAACAGCGCAGCAGA
AACGCTGACCCGCCTGCACAACATGGGCATTCCCGGCTTCAACATCGCGACCTCGGTGAGCCTGATCATTGCCCAGCGCC
TGGCGCGAAAACTCTGTAGCCACTGCAAGAAACCCATCGAGATCCCCCGGGAGACCCTGATCAAGGAAGGTTTCCCCGAG
GAACGCATCGGCCATTTCACGATCTATGAGCCCTCTGGTTGCGATCACTGCAACGGCGGGTACAAGGGTCGCGTGGGAAT
TTATGAAGTGGTGAAGAACACCCCCGACCTGCAACGGCTGATCATGGCCGAGGGCAACTCGCTGGAAATCGATACCCAGA
TGCGCCGGGACGGCTTCAACGACCTGCGCACCTCGGGCCTGATCAAGGCCATGCAAGGCATCACCAGCCTTGAAGAAATC
AACCGGGTCACCAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

58.407

99.823

0.583
  pilB Acinetobacter baumannii D1279779

57.801

99.647

0.576
  pilB Legionella pneumophila strain ERS1305867

56.36

100

0.564
  pilB Vibrio cholerae strain A1552

50.794

100

0.509
  pilB Vibrio campbellii strain DS40M4

48.438

100

0.493
  pilB Vibrio parahaemolyticus RIMD 2210633

49.554

99.117

0.491
  pilF Neisseria gonorrhoeae MS11

48.845

99.47

0.486
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.434

93.64

0.369
  pilF Thermus thermophilus HB27

36.918

98.587

0.364