Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OU800_RS04085 Genome accession   NZ_CP113519
Coordinates   900054..901757 (-) Length   567 a.a.
NCBI ID   WP_268181385.1    Uniprot ID   -
Organism   Pseudomonas sp. GOM7     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 895054..906757
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU800_RS04065 (OU800_04065) - 895454..896266 (+) 813 WP_268181377.1 YdcF family protein -
  OU800_RS04070 (OU800_04070) lnt 896345..897868 (-) 1524 WP_268181379.1 apolipoprotein N-acyltransferase -
  OU800_RS04075 (OU800_04075) pilD 897964..898833 (-) 870 WP_268181381.1 A24 family peptidase Machinery gene
  OU800_RS04080 (OU800_04080) pilC 898834..900051 (-) 1218 WP_268181383.1 type II secretion system F family protein Machinery gene
  OU800_RS04085 (OU800_04085) pilB 900054..901757 (-) 1704 WP_268181385.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OU800_RS04090 (OU800_04090) - 901980..902474 (+) 495 WP_268181386.1 pilin -
  OU800_RS04095 (OU800_04095) tfpZ 902628..903359 (+) 732 WP_268181387.1 TfpX/TfpZ family type IV pilin accessory protein -
  OU800_RS04100 (OU800_04100) nadC 903679..904527 (-) 849 WP_268181388.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 567 a.a.        Molecular weight: 62677.54 Da        Isoelectric Point: 5.6176

>NTDB_id=762674 OU800_RS04085 WP_268181385.1 900054..901757(-) (pilB) [Pseudomonas sp. GOM7]
MNDSISLSGLARQLAQTGLMDEKAAQQAQQQAQRNKLSLVTYLVQNRLVKSRALAELASEQFGVSFFDLNAIDKESQPRD
LVSEKLVRQHRVLPLWRRGNKLFVGLSDPTNHQAVTDIQFSTGLNTEAILVEDDKLGDAIDKFFESSNSGMEDLADVDLD
GLDVEAVNDDKPVGETGADADDAPVVRFVNKMLLDAIKGGSSDLHFEPYEKTYRVRFRTDGILHEVARPPIQLAPRISAR
LKVMAALDISERRKPQDGRIKMRVSKNKAIDFRVNTLPTLWGEKIVMRILDPTSAQMGIDALGYEEEQKELYMNALKQPQ
GMILVTGPTGSGKTVSLYTGLNILNTPDVNISTAEDPVEINLEGINQVNVNPKQGMDFTQALRAFLRQDPDIIMVGEIRD
LDTASIAVKAAQTGHMVMSTLHTNSAAETLTRLRNMGVPSFNIATSVNLIIAQRLARKLCNHCKKEVDVPREALLAEGFP
EDKIGTFKIYGPVGCENCKGGYKGRVGIYEVVKNTPNLQRIIMEEGNSIDIATQMRKDGFNDLRTSALLKAMQGVTSLEE
VNRVTKD

Nucleotide


Download         Length: 1704 bp        

>NTDB_id=762674 OU800_RS04085 WP_268181385.1 900054..901757(-) (pilB) [Pseudomonas sp. GOM7]
ATGAACGACAGCATTTCCCTTTCCGGCCTGGCCAGGCAGTTGGCGCAAACCGGGTTGATGGACGAAAAGGCCGCCCAGCA
AGCGCAACAGCAAGCCCAGCGCAACAAGCTGTCGCTGGTGACCTATCTGGTGCAGAACAGGCTGGTCAAGAGCCGGGCCC
TGGCCGAGCTGGCCTCGGAGCAGTTCGGCGTATCCTTCTTCGACCTCAACGCCATCGACAAGGAAAGTCAGCCACGTGAC
CTGGTCAGCGAAAAGCTGGTACGCCAGCATCGAGTGCTGCCGTTGTGGCGGCGGGGCAATAAGTTGTTCGTCGGGCTTTC
CGACCCCACCAACCACCAGGCCGTCACCGACATCCAGTTCAGTACAGGCCTCAACACCGAAGCCATTCTGGTCGAGGATG
ACAAGCTCGGCGATGCCATCGACAAGTTCTTCGAGTCATCCAATAGCGGCATGGAGGATCTGGCCGATGTAGATCTGGAT
GGCCTGGATGTGGAGGCGGTCAATGATGACAAGCCGGTAGGTGAAACTGGCGCCGATGCCGACGACGCGCCAGTGGTGCG
CTTCGTCAACAAGATGCTGCTGGATGCCATCAAGGGAGGCTCTTCCGACCTGCACTTCGAGCCCTACGAAAAGACCTACC
GCGTGCGCTTTCGTACCGACGGTATCCTGCATGAAGTAGCCCGCCCACCCATCCAGTTGGCGCCACGCATTTCCGCGCGC
CTCAAGGTGATGGCCGCACTGGATATTTCCGAGCGGCGCAAGCCGCAGGATGGCCGGATCAAGATGCGCGTATCAAAGAA
CAAGGCCATCGACTTTCGCGTCAACACACTGCCCACCCTGTGGGGCGAGAAGATCGTTATGCGGATTCTCGACCCCACCA
GCGCACAGATGGGTATCGATGCCCTCGGCTACGAAGAAGAGCAGAAAGAGCTCTATATGAATGCCCTCAAGCAACCCCAG
GGCATGATCCTGGTCACCGGCCCCACCGGCTCGGGCAAGACGGTGTCGCTCTATACCGGCCTGAATATTCTCAATACGCC
GGACGTGAACATTTCCACCGCCGAAGACCCGGTGGAAATCAACCTCGAAGGTATCAACCAGGTCAACGTCAATCCCAAGC
AGGGTATGGACTTCACCCAGGCGCTACGCGCCTTCCTGCGTCAGGATCCGGACATCATCATGGTGGGTGAGATCCGCGAT
CTGGATACCGCCTCCATCGCCGTCAAGGCCGCGCAGACCGGGCACATGGTAATGTCCACCCTGCATACCAACAGCGCCGC
GGAAACCCTCACGCGCCTGCGCAACATGGGTGTGCCCTCCTTCAATATCGCCACCTCGGTGAACCTGATCATCGCCCAGC
GCCTGGCGCGCAAGCTGTGCAACCACTGTAAGAAGGAAGTGGACGTACCGCGCGAAGCGCTGCTGGCTGAAGGCTTCCCC
GAGGACAAGATCGGCACCTTCAAGATCTACGGCCCGGTTGGCTGCGAAAATTGCAAGGGCGGTTACAAGGGTCGTGTCGG
TATTTATGAAGTGGTTAAAAACACGCCCAACCTGCAGCGCATTATCATGGAAGAAGGCAACTCCATCGATATCGCCACAC
AGATGCGTAAAGACGGCTTCAATGATCTGCGCACCTCCGCTTTGCTCAAGGCCATGCAAGGGGTTACCAGCCTTGAGGAA
GTCAACCGCGTAACCAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(43-147)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

58.885

100

0.596
  pilB Acinetobacter baylyi ADP1

58.437

99.295

0.58
  pilB Legionella pneumophila strain ERS1305867

54.513

99.647

0.543
  pilB Vibrio parahaemolyticus RIMD 2210633

49.033

100

0.492
  pilB Vibrio cholerae strain A1552

49.643

98.765

0.49
  pilF Neisseria gonorrhoeae MS11

48.668

99.295

0.483
  pilB Vibrio campbellii strain DS40M4

47.895

100

0.481
  pilF Thermus thermophilus HB27

37.478

99.295

0.372
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

39.36

93.651

0.369