Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OU800_RS02520 Genome accession   NZ_CP113519
Coordinates   556068..558188 (+) Length   706 a.a.
NCBI ID   WP_268180926.1    Uniprot ID   -
Organism   Pseudomonas sp. GOM7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 551068..563188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU800_RS02500 (OU800_02500) pilM 553233..554297 (+) 1065 WP_268180919.1 pilus assembly protein PilM Machinery gene
  OU800_RS02505 (OU800_02505) pilN 554297..554869 (+) 573 WP_268180921.1 type 4a pilus biogenesis protein PilN -
  OU800_RS02510 (OU800_02510) pilO 554866..555486 (+) 621 WP_268180922.1 type 4a pilus biogenesis protein PilO -
  OU800_RS02515 (OU800_02515) pilP 555486..556010 (+) 525 WP_268180924.1 type 4a pilus biogenesis lipoprotein PilP -
  OU800_RS02520 (OU800_02520) pilQ 556068..558188 (+) 2121 WP_268180926.1 type IV pilus secretin PilQ Machinery gene
  OU800_RS02525 (OU800_02525) aroK 558194..558712 (+) 519 WP_268180928.1 shikimate kinase AroK -
  OU800_RS02530 (OU800_02530) aroB 558764..559867 (+) 1104 WP_268180930.1 3-dehydroquinate synthase -
  OU800_RS02535 (OU800_02535) - 559878..561446 (+) 1569 WP_268180932.1 AAA family ATPase -

Sequence

Protein


Download         Length: 706 a.a.        Molecular weight: 76275.52 Da        Isoelectric Point: 4.7878

>NTDB_id=762669 OU800_RS02520 WP_268180926.1 556068..558188(+) (pilQ) [Pseudomonas sp. GOM7]
MNGSLSCLSAALLMAFLSSSALAANLQGMDVASLPGDRVELKLSFDEPVAVPRGYTIEQPARIALDLPGVANKLGAKNRE
LGAGNARSVTIVEAKDRTRLIVNLGTLAPYSTRVEGNDLYVLVGESTAAPASRPAVAATPVAPAPSKKVYGAPTRAVKNI
DFQRGEQGEGNVVITLSDPSVSPDIQEQGGKIRLDFAKTELPESLRVKLDVKDFATPVQFVSATGTADKTSILIEPVGQY
DYLAYQTDDKLTLSVKPLTQEDTERRSAERFVYKGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVSGNITLRLQNVP
WDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELEAQKQIAELAPLRRELIQVNYAKASDMAKLFQSVTSADGAA
DDRGSITVDDRTNSIIAYQTQERLDELRRIIAQLDIPVRQVMIEARIVEANVDYDKALGVRWGGSVRNGRWDVSGKDGVQ
AFDEDTGQRAGFVGTDEIGRTTLDDGLARLPFVDMGAQNSTSGIAIGYLGSSIVLDLQLSAMEKTGNGEIVSQPKVVTSD
KETAKILKGQEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDAPDFARAINGVPPINKNEVNAKVLV
NDGETIVIGGVFSNTQTRSVEKVPFLGDLPFIGRVFRRDVVADNKAELLVFITPRIMNNQAIAVNR

Nucleotide


Download         Length: 2121 bp        

>NTDB_id=762669 OU800_RS02520 WP_268180926.1 556068..558188(+) (pilQ) [Pseudomonas sp. GOM7]
ATGAATGGCTCTCTTTCTTGCTTGAGTGCCGCCTTATTGATGGCGTTCTTATCGTCCTCGGCTTTGGCGGCTAATCTTCA
AGGTATGGATGTCGCGAGCTTGCCGGGTGATCGGGTCGAGCTGAAATTGTCCTTCGACGAGCCTGTGGCAGTGCCGCGTG
GCTATACCATCGAGCAGCCTGCGCGTATCGCCCTGGATCTGCCAGGCGTTGCCAACAAGCTCGGCGCGAAGAACCGGGAG
CTTGGCGCTGGCAATGCCCGTAGCGTGACCATAGTCGAGGCCAAGGATCGCACGCGATTGATCGTCAATCTCGGCACCCT
GGCGCCCTACAGTACGCGTGTGGAGGGTAACGATCTCTACGTGCTGGTCGGTGAATCCACTGCTGCCCCGGCCAGCCGAC
CGGCCGTGGCGGCCACCCCGGTCGCTCCAGCTCCGAGCAAGAAGGTCTATGGCGCACCGACCAGGGCCGTGAAGAACATC
GATTTCCAGCGTGGTGAACAAGGTGAGGGAAATGTCGTCATCACGCTTTCCGATCCCTCCGTCAGTCCTGATATTCAGGA
GCAGGGTGGCAAGATTCGTCTGGACTTCGCCAAGACCGAACTGCCCGAGTCCCTGCGCGTAAAGCTCGATGTGAAGGACT
TCGCTACGCCGGTGCAGTTCGTCAGTGCGACCGGCACAGCCGACAAGACCAGCATCCTGATCGAGCCGGTGGGGCAGTAC
GATTATCTCGCCTATCAGACGGACGACAAGCTCACCCTGAGCGTCAAGCCGTTGACCCAGGAAGATACCGAGCGTCGCAG
TGCCGAGCGCTTCGTCTACAAGGGCGAGAAGCTGTCGCTGAACTTCCAGGACATCGACGTGCGCTCCGTGCTGCAGTTGA
TCGCGGACTTCACCAACTTGAACCTGGTTGCCAGTGATACCGTTTCCGGCAACATCACCCTGCGTTTGCAGAACGTGCCC
TGGGATCAGGCGTTGGATCTGGTGCTCAAGACCAAGGGGCTCGACAAGCGTCAGGTCGGCAACGTGTTGTTGGTTGCACC
GGCGGATGAAATTGCCGCGCGCGAGCGCCAGGAGCTGGAAGCGCAGAAGCAGATTGCCGAACTGGCACCGCTGCGTCGTG
AGTTGATCCAGGTGAATTATGCCAAGGCGAGCGATATGGCCAAGCTATTCCAGTCGGTGACCAGTGCCGATGGTGCAGCC
GATGATCGTGGTTCCATTACGGTCGATGACCGAACCAACAGCATCATTGCCTACCAGACTCAGGAGCGTCTGGACGAACT
GCGGCGCATCATTGCCCAGCTCGATATCCCGGTGCGTCAGGTGATGATCGAGGCGCGCATCGTCGAGGCCAATGTCGATT
ATGACAAGGCGTTGGGTGTGCGCTGGGGAGGCAGTGTGCGCAATGGCCGCTGGGATGTCAGTGGCAAGGACGGCGTGCAG
GCTTTCGATGAAGATACTGGTCAGCGGGCTGGCTTTGTCGGTACGGACGAGATTGGTCGCACGACCTTGGATGATGGCTT
GGCTCGACTTCCATTCGTTGACATGGGAGCACAGAACAGCACTTCCGGTATTGCGATAGGCTACTTGGGAAGTAGTATCG
TCCTGGATCTTCAGCTCTCGGCCATGGAGAAGACGGGGAACGGCGAGATCGTCTCTCAGCCCAAGGTAGTCACCTCCGAT
AAGGAAACGGCCAAGATTCTCAAAGGCCAGGAAGTTCCCTACCAAGAGGCCAGCTCCAGCGGTGCTACCAGCACTTCGTT
CAAGGAGGCGGCGCTTTCCCTTGAGGTCACCCCACAGATTACCCCTGATAACCGCATCATCATGGAGGTCAAGGTCACCA
AGGATGCGCCGGATTTTGCCAGGGCGATCAATGGCGTACCGCCCATCAACAAGAACGAAGTGAATGCGAAGGTTCTGGTT
AACGACGGTGAAACCATCGTGATTGGTGGGGTGTTCTCCAATACCCAGACCCGATCCGTCGAGAAAGTCCCTTTCCTGGG
GGATCTGCCTTTCATCGGCCGTGTTTTCCGGCGCGATGTCGTCGCTGACAACAAAGCCGAGTTGCTGGTTTTCATCACTC
CGCGCATCATGAACAACCAAGCCATTGCCGTGAATCGATGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(39-120)

Secretin and TonB N terminus short domain (STN)

(301-347)

Bacterial type II/III secretion system short domain (Secretin_N)

(375-440)

Bacterial type II and III secretion system protein (Secretin)

(541-697)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.028

100

0.755
  pilQ Acinetobacter baumannii strain A118

40.541

100

0.425
  pilQ Acinetobacter baumannii D1279779

40.541

100

0.425
  comQ Acinetobacter baylyi ADP1

40.909

100

0.421
  pilQ Legionella pneumophila strain Lp02

37.464

99.433

0.373
  pilQ Legionella pneumophila strain ERS1305867

37.771

97.875

0.37