Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   OU800_RS02500 Genome accession   NZ_CP113519
Coordinates   553233..554297 (+) Length   354 a.a.
NCBI ID   WP_268180919.1    Uniprot ID   -
Organism   Pseudomonas sp. GOM7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 548233..559297
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OU800_RS02490 (OU800_02490) - 549311..550579 (+) 1269 WP_268180915.1 malic enzyme-like NAD(P)-binding protein -
  OU800_RS02495 (OU800_02495) - 550640..553051 (-) 2412 WP_268180917.1 penicillin-binding protein 1A -
  OU800_RS02500 (OU800_02500) pilM 553233..554297 (+) 1065 WP_268180919.1 pilus assembly protein PilM Machinery gene
  OU800_RS02505 (OU800_02505) pilN 554297..554869 (+) 573 WP_268180921.1 type 4a pilus biogenesis protein PilN -
  OU800_RS02510 (OU800_02510) pilO 554866..555486 (+) 621 WP_268180922.1 type 4a pilus biogenesis protein PilO -
  OU800_RS02515 (OU800_02515) pilP 555486..556010 (+) 525 WP_268180924.1 type 4a pilus biogenesis lipoprotein PilP -
  OU800_RS02520 (OU800_02520) pilQ 556068..558188 (+) 2121 WP_268180926.1 type IV pilus secretin PilQ Machinery gene
  OU800_RS02525 (OU800_02525) aroK 558194..558712 (+) 519 WP_268180928.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 37772.12 Da        Isoelectric Point: 4.4647

>NTDB_id=762668 OU800_RS02500 WP_268180919.1 553233..554297(+) (pilM) [Pseudomonas sp. GOM7]
MLGLFTKKANTLLGIDISSTSVKLLELSRSGSRYKVEAYAVEPLPPNAVVEKNIAELEGVGQALSRVLSKAKTSVKSAAV
AVAGSAVITKSIEMEAGLSDDELENQLKIEADQYIPYPLEEVAIDFEVQGPAARNPERVEVLLAACRKENVEVREAALAL
AGLTAKVVDVEAYALERAYALLESQLGAGHDELTVAIVDIGATMTTLSVLHNGRTIYTREQLFGGKQLTEEIQRRYGLSV
EEAGLAKKQGGLPDDYDAEVLQPFKEAVVQQVSRSLQFFFAAGQFSDVDYVLLAGGTASIPDLDRLIQQKIGTQTLVANP
FADMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=762668 OU800_RS02500 WP_268180919.1 553233..554297(+) (pilM) [Pseudomonas sp. GOM7]
GTGCTAGGGCTCTTCACTAAGAAAGCGAATACGCTGCTGGGGATCGATATCAGCTCGACCTCGGTCAAGCTCCTCGAACT
GAGTCGCTCGGGAAGCCGCTACAAGGTAGAGGCTTACGCAGTCGAGCCGCTCCCGCCAAATGCTGTCGTGGAAAAGAACA
TCGCCGAGCTGGAGGGGGTGGGGCAGGCGCTGTCGCGAGTGTTGAGCAAGGCCAAGACGAGCGTCAAATCAGCCGCGGTG
GCCGTTGCCGGCTCCGCCGTGATCACCAAGAGCATCGAGATGGAGGCCGGGCTGTCGGACGACGAGCTGGAGAACCAGCT
CAAGATCGAAGCCGACCAGTACATTCCTTATCCGCTCGAGGAAGTGGCGATCGATTTCGAGGTTCAGGGCCCGGCTGCAC
GCAATCCCGAGCGGGTCGAAGTGCTGCTGGCCGCCTGCCGCAAGGAGAACGTCGAAGTGCGTGAGGCCGCCCTCGCCCTG
GCAGGACTGACCGCCAAGGTGGTGGATGTCGAAGCCTATGCCCTCGAGCGTGCTTACGCGCTGCTGGAGAGTCAGTTGGG
CGCCGGTCATGACGAGCTCACCGTGGCCATCGTCGATATCGGTGCCACCATGACCACGCTGAGCGTGCTGCACAACGGTC
GCACCATCTATACCCGCGAGCAGTTGTTCGGTGGCAAGCAGCTCACCGAAGAAATTCAGCGACGCTACGGGCTGTCCGTC
GAGGAAGCCGGGTTGGCCAAGAAGCAGGGTGGCCTGCCGGACGACTACGATGCCGAAGTCCTGCAGCCATTCAAGGAAGC
CGTGGTGCAGCAGGTGTCGCGCTCGCTGCAGTTCTTCTTCGCTGCCGGTCAGTTCAGTGATGTCGATTACGTCCTGCTGG
CAGGCGGAACGGCTTCCATTCCCGACCTTGACCGTCTCATCCAGCAGAAAATCGGTACGCAAACTCTAGTGGCCAACCCT
TTTGCCGATATGGCTCTGGGTAGCAAGGTCAACGCAGGTGCGTTGGCCAGTGATGCGCCGGCACTGATGATTGCCTGCGG
TCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter nosocomialis M2

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

53.672

100

0.537
  pilM Legionella pneumophila strain ERS1305867

46.328

100

0.463