Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   NM686_RS17095 Genome accession   NZ_CP113517
Coordinates   3628779..3629225 (+) Length   148 a.a.
NCBI ID   WP_407942389.1    Uniprot ID   -
Organism   Methylomonas rapida strain MP1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3623779..3634225
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  NM686_RS17055 (NM686_017055) - 3624573..3625934 (+) 1362 WP_255189054.1 sigma-54 dependent transcriptional regulator -
  NM686_RS17060 (NM686_017060) rplM 3626014..3626442 (+) 429 WP_255189055.1 50S ribosomal protein L13 -
  NM686_RS17065 (NM686_017065) rpsI 3626458..3626847 (+) 390 WP_255189056.1 30S ribosomal protein S9 -
  NM686_RS17070 (NM686_017070) - 3626981..3627280 (+) 300 WP_255189057.1 BrnT family toxin -
  NM686_RS17075 (NM686_017075) - 3627264..3627533 (+) 270 WP_255189058.1 BrnA antitoxin family protein -
  NM686_RS17080 (NM686_017080) - 3627648..3628160 (-) 513 WP_255189059.1 tetratricopeptide repeat protein -
  NM686_RS17085 (NM686_017085) - 3628441..3628767 (+) 327 WP_255189060.1 hypothetical protein -
  NM686_RS17095 (NM686_017095) pilA 3628779..3629225 (+) 447 WP_407942389.1 pilin Machinery gene
  NM686_RS17100 (NM686_017100) - 3629307..3631136 (+) 1830 WP_255189061.1 hypothetical protein -
  NM686_RS17105 (NM686_017105) - 3631203..3632588 (-) 1386 WP_255187523.1 IS4 family transposase -
  NM686_RS17110 (NM686_017110) cobD 3632771..3633793 (-) 1023 WP_255189062.1 threonine-phosphate decarboxylase CobD -

Sequence

Protein


Download         Length: 148 a.a.        Molecular weight: 15559.01 Da        Isoelectric Point: 9.5829

>NTDB_id=762655 NM686_RS17095 WP_407942389.1 3628779..3629225(+) (pilA) [Methylomonas rapida strain MP1]
MKKVQQGFTLIELMIVVAIIGILAAIAIPAYQDYVVRAKVTEGLSLASSAKVTVSENAMNGLSDLTKGWTPPQATDKVTS
VTVNSTNGQITITYTPSAGNGTLFLTPWEGAVGTVLAAGTVPTKPIVWTCSNSTATLPNKFRPANCRF

Nucleotide


Download         Length: 447 bp        

>NTDB_id=762655 NM686_RS17095 WP_407942389.1 3628779..3629225(+) (pilA) [Methylomonas rapida strain MP1]
ATGAAAAAAGTACAACAAGGCTTTACCTTGATCGAATTGATGATCGTCGTGGCGATCATTGGTATCTTGGCAGCTATCGC
GATTCCGGCTTATCAAGATTACGTTGTGAGAGCCAAAGTCACCGAAGGTTTGAGCTTGGCTTCATCCGCCAAAGTAACCG
TATCGGAAAACGCCATGAACGGTTTATCTGATTTAACCAAAGGTTGGACGCCTCCACAAGCAACTGACAAGGTTACTTCA
GTTACTGTCAACTCGACTAATGGGCAAATCACGATTACTTATACGCCTTCCGCAGGAAACGGCACCCTGTTTTTAACTCC
CTGGGAAGGAGCTGTCGGAACTGTCTTAGCTGCCGGAACAGTTCCAACCAAGCCAATCGTTTGGACATGTTCCAACTCAA
CTGCTACTCTCCCAAACAAATTTAGACCGGCCAACTGCCGCTTCTAA

Domains

Predicted by InterproScan.

Pilin (bacterial filament) (Pilin)

(36-147)

Prokaryotic N-terminal methylation motif (N_methyl)

(3-27)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Ralstonia pseudosolanacearum GMI1000

54.321

100

0.595
  pilA2 Legionella pneumophila strain ERS1305867

55.102

99.324

0.547
  pilA2 Legionella pneumophila str. Paris

55.102

99.324

0.547
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

35.417

100

0.459
  comP Acinetobacter baylyi ADP1

41.975

100

0.459
  pilA/pilA1 Eikenella corrodens VA1

38.272

100

0.419
  pilA Acinetobacter baumannii strain A118

39.583

97.297

0.385