Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OUY36_RS03210 Genome accession   NZ_CP113258
Coordinates   710362..712479 (+) Length   705 a.a.
NCBI ID   WP_156245536.1    Uniprot ID   -
Organism   Stutzerimonas sp. R40042     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Genomic island 707528..715744 710362..712479 within 0


Gene organization within MGE regions


Location: 707528..715744
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OUY36_RS03190 (OUY36_03190) comM 707528..708592 (+) 1065 WP_161611797.1 pilus assembly protein PilM Machinery gene
  OUY36_RS03195 (OUY36_03195) - 708592..709164 (+) 573 WP_063539613.1 PilN domain-containing protein -
  OUY36_RS03200 (OUY36_03200) pilO 709161..709784 (+) 624 WP_110778197.1 type 4a pilus biogenesis protein PilO -
  OUY36_RS03205 (OUY36_03205) pilP 709781..710308 (+) 528 WP_156245535.1 type 4a pilus biogenesis lipoprotein PilP -
  OUY36_RS03210 (OUY36_03210) pilQ 710362..712479 (+) 2118 WP_156245536.1 type IV pilus secretin PilQ Machinery gene
  OUY36_RS03215 (OUY36_03215) aroK 712485..713030 (+) 546 WP_161611796.1 shikimate kinase AroK -
  OUY36_RS03220 (OUY36_03220) aroB 713053..714156 (+) 1104 WP_161611795.1 3-dehydroquinate synthase -
  OUY36_RS03225 (OUY36_03225) - 714167..715744 (+) 1578 WP_267936746.1 AAA family ATPase -

Sequence

Protein


Download         Length: 705 a.a.        Molecular weight: 75960.97 Da        Isoelectric Point: 4.7802

>NTDB_id=761333 OUY36_RS03210 WP_156245536.1 710362..712479(+) (pilQ) [Stutzerimonas sp. R40042]
MNSTLSRLGISVLVAFVSPAVLAANLNALDVAALPGDRVELKLAFDEPVAPPRGYTLEQPARIALDLPGVSNNLGAKNRE
LGVGNARSVTVVEAQGRTRLIVNLTSLVPYSTRVDGKNLFVVLGEHAGASGPAAVSAQPSAVAQPSVTTAPAKVVAVGRA
IDNIDFRRGDDGAGNVIITLSDPSISSTIEEQGGKIRVNFAKAQLPESLRVRLDVQDFATPVRFVDSASQAGGASIAIEP
TGRYDYLAYQTDNKLTISVKPLTEDEAEKRKADRFAYSGEKLSLNFQDIDVRSVLQLIADFTDLNLVASDTVSGNITLRL
QNVPWDQALDLVLKTKGLDKRQVGNVLLVAPADEIAARERQELESQRQIAELAPLRREVVQVNYAKAADIARLFQSVTDN
GGMAEERGSIAVDDRTNNIIAYQTQERLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGNLWSGDKWNVWG
KDGDLGVEDGEEGQVGRFPGIRETPLNTPFVDMGAVGATSGFGLGFVTNNAILDLQLSAMEKTGNGEVVSQPKVVTADKE
TAKILKGSEVPYQEASSSGATSTSFKEAALSLEVTPQITPDNRIIMEVKVTKDEPDFSQAATTGVPAIRKNEVNAKVLVT
DGETIVIGGVFSNTQSKSVDKVPFLGDLPFLGRLFRRDLVQDRKSELLVFITPRIMNNQAIQASR

Nucleotide


Download         Length: 2118 bp        

>NTDB_id=761333 OUY36_RS03210 WP_156245536.1 710362..712479(+) (pilQ) [Stutzerimonas sp. R40042]
ATGAACAGTACTCTCTCGCGTCTCGGCATTTCAGTGCTTGTGGCGTTCGTCTCGCCGGCTGTACTGGCAGCCAATCTCAA
TGCCTTGGATGTGGCCGCTCTGCCGGGCGATCGAGTCGAACTCAAGCTGGCTTTCGACGAGCCCGTTGCGCCGCCGCGTG
GTTACACCCTTGAGCAGCCTGCTCGTATTGCCCTGGATCTGCCGGGTGTATCGAACAACCTGGGGGCGAAGAATCGTGAG
CTCGGGGTTGGTAATGCGCGTAGCGTTACGGTCGTTGAGGCGCAAGGACGTACTCGGTTGATCGTGAACCTCACTTCGCT
GGTGCCCTATTCCACTCGAGTGGATGGCAAGAATCTGTTCGTCGTACTGGGCGAACATGCCGGCGCGAGTGGCCCAGCAG
CGGTCTCAGCGCAGCCGAGCGCAGTGGCGCAGCCATCTGTTACGACGGCTCCCGCGAAGGTGGTGGCTGTTGGTAGGGCC
ATTGACAACATTGACTTTCGGCGTGGCGATGATGGAGCCGGTAATGTGATTATTACGCTATCCGATCCATCAATCAGCTC
GACCATTGAGGAGCAGGGCGGAAAAATTCGCGTAAATTTCGCCAAGGCTCAGTTGCCGGAATCGCTGCGTGTGCGCCTGG
ATGTCCAAGACTTCGCTACGCCTGTCAGGTTCGTTGATTCCGCTTCTCAGGCAGGTGGTGCGAGCATCGCAATCGAGCCG
ACCGGGCGATATGACTATTTGGCCTACCAGACCGACAACAAGCTGACTATCAGCGTAAAACCTCTTACGGAAGACGAAGC
GGAAAAGCGTAAGGCTGATCGATTTGCTTATTCTGGCGAGAAGCTGTCGCTTAATTTCCAAGACATCGACGTTCGCTCGG
TATTGCAGCTAATCGCTGATTTCACGGATCTAAATCTGGTCGCAAGCGATACCGTTAGCGGCAATATCACGCTGCGTCTG
CAGAACGTGCCGTGGGATCAGGCTCTGGACTTGGTGCTTAAGACCAAGGGGTTGGACAAGCGCCAGGTCGGTAACGTGCT
GCTGGTGGCTCCTGCCGACGAAATTGCCGCACGTGAACGTCAGGAGCTTGAGTCTCAGCGGCAGATTGCCGAACTGGCTC
CGCTTCGCCGTGAGGTGGTGCAGGTTAACTATGCCAAGGCCGCTGACATCGCCAGGCTATTTCAGTCGGTAACGGATAAT
GGCGGGATGGCGGAAGAGCGGGGGTCTATTGCGGTTGATGACCGCACCAACAATATCATTGCCTACCAGACTCAGGAGCG
TCTCGATGAGTTGCGTCGTATCGTGGCTCAACTGGATATTCCGGTTCGGCAGGTAATGATTGAGGCGCGCATAGTTGAGG
CAAACGTCGACTACGATAAGAGCCTTGGTGTTCGTTGGGGCGGCAACTTATGGTCGGGAGATAAATGGAACGTTTGGGGT
AAAGACGGCGATCTTGGCGTAGAGGATGGAGAAGAAGGACAGGTCGGAAGGTTTCCCGGCATTCGGGAAACCCCGCTTAA
CACCCCTTTCGTCGATATGGGCGCAGTGGGAGCAACGTCTGGATTTGGCTTAGGCTTCGTTACGAATAATGCAATTTTAG
ATCTACAGCTGTCTGCCATGGAGAAAACCGGTAATGGCGAGGTTGTCTCGCAGCCTAAGGTAGTCACCGCAGACAAAGAA
ACTGCAAAGATCCTCAAGGGCTCCGAAGTTCCTTATCAGGAGGCGAGCTCCAGTGGTGCAACGAGCACGTCGTTCAAGGA
GGCAGCTCTCTCGCTGGAAGTGACTCCGCAGATCACACCGGATAATCGCATCATCATGGAGGTCAAGGTCACTAAGGATG
AGCCAGACTTCTCTCAGGCAGCGACTACCGGAGTACCGGCCATTCGCAAGAATGAGGTTAACGCAAAGGTTCTGGTAACT
GATGGTGAGACGATCGTAATCGGTGGCGTTTTCTCCAATACGCAGAGCAAGTCAGTGGACAAGGTGCCGTTCCTAGGGGA
TCTCCCGTTCTTGGGTCGCCTGTTCCGTCGTGATCTGGTGCAGGATCGAAAGTCTGAACTGCTTGTGTTCATCACGCCGA
GGATCATGAATAATCAGGCGATTCAAGCCAGTCGCTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(38-123)

Secretin and TonB N terminus short domain (STN)

(305-351)

Bacterial type II and III secretion system protein (Secretin)

(539-696)

Bacterial type II/III secretion system short domain (Secretin_N)

(378-444)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.992

100

0.755
  comQ Acinetobacter baylyi ADP1

41.16

100

0.413
  pilQ Acinetobacter baumannii strain A118

38.42

100

0.407
  pilQ Acinetobacter baumannii D1279779

38.42

100

0.407
  pilQ Legionella pneumophila strain Lp02

37.427

97.021

0.363
  pilQ Legionella pneumophila strain ERS1305867

37.611

96.17

0.362