Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   QJ951_RS24950 Genome accession   NZ_CP124668
Coordinates   5354518..5354982 (-) Length   154 a.a.
NCBI ID   WP_023098059.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2021CK-01445     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5349518..5359982
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  QJ951_RS24935 (QJ951_24935) nadC 5352028..5352876 (+) 849 WP_023098057.1 carboxylating nicotinate-nucleotide diphosphorylase -
  QJ951_RS24945 (QJ951_24945) - 5353057..5354436 (-) 1380 WP_226342245.1 O-antigen ligase family protein -
  QJ951_RS24950 (QJ951_24950) pilA 5354518..5354982 (-) 465 WP_023098059.1 pilin Machinery gene
  QJ951_RS24955 (QJ951_24955) pilB 5355213..5356913 (+) 1701 WP_023098060.1 type IV-A pilus assembly ATPase PilB Machinery gene
  QJ951_RS24960 (QJ951_24960) pilC 5356917..5358134 (+) 1218 WP_003161763.1 type II secretion system F family protein Machinery gene
  QJ951_RS24965 (QJ951_24965) pilD 5358135..5359007 (+) 873 WP_023084740.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  QJ951_RS24970 (QJ951_24970) coaE 5359004..5359615 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  QJ951_RS24975 (QJ951_24975) yacG 5359612..5359812 (+) 201 WP_017148551.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16106.47 Da        Isoelectric Point: 9.0145

>NTDB_id=760984 QJ951_RS24950 WP_023098059.1 5354518..5354982(-) (pilA) [Pseudomonas aeruginosa strain 2021CK-01445]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSKTPTKDQYDIGFTG
STLLKGSEKGQITVKNGTLPTVQLEATLDGSSGAAIKGAVITVSRTADGVWGCSISSTPTNWKPNYAPSNCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=760984 QJ951_RS24950 WP_023098059.1 5354518..5354982(-) (pilA) [Pseudomonas aeruginosa strain 2021CK-01445]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTCGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATACCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCTGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAAGAAATTGTTTCCAGTAAGACTCCTACCAAGGACCAATATGATATTGGATTTACCGGT
TCTACCTTGCTGAAAGGGTCGGAGAAAGGTCAGATTACTGTGAAAAATGGTACTCTGCCTACTGTTCAGTTGGAAGCCAC
TCTGGATGGTTCTTCCGGTGCCGCTATTAAAGGTGCAGTTATTACCGTTTCGCGTACCGCTGATGGGGTGTGGGGTTGCT
CGATCAGTAGCACTCCTACTAACTGGAAACCAAACTATGCCCCCTCTAACTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Pilin (bacterial filament) (Pilin)

(35-151)

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Pseudomonas aeruginosa PAK

42.857

100

0.448
  pilA/pilAI Pseudomonas stutzeri DSM 10701

47.26

94.805

0.448
  pilA Acinetobacter baumannii strain A118

45.89

94.805

0.435
  comP Acinetobacter baylyi ADP1

39.264

100

0.416
  pilA/pilAII Pseudomonas stutzeri DSM 10701

38.776

95.455

0.37
  pilA Vibrio parahaemolyticus RIMD 2210633

41.007

90.26

0.37