Detailed information NTG0760204

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: P7I93_RS29865
• Genome accession: NZ_CP124658
• Coordinates: 6282736..6283788 (-)
• Length: 350 a.a.
• NCBI ID: WP_034055307.1
• Uniprot ID: -
• Organism: Pseudomonas aeruginosa strain 2022CK-00068
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 6277736..6288788

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
P7I93_RS29840 (P7I93_29840)	aroK	6278271..6278789 (-)	519	WP_003095833.1	shikimate kinase AroK	-
P7I93_RS29845 (P7I93_29845)	pilQ	6278801..6280945 (-)	2145	WP_003095835.1	type 4a pilus secretin PilQ	Machinery gene
P7I93_RS29850 (P7I93_29850)	comL/pilP	6280999..6281523 (-)	525	WP_003095836.1	type 4a pilus biogenesis lipoprotein PilP	Machinery gene
P7I93_RS29855 (P7I93_29855)	pilO	6281520..6282143 (-)	624	WP_003103268.1	type 4a pilus biogenesis protein PilO	Machinery gene
P7I93_RS29860 (P7I93_29860)	pilN	6282140..6282736 (-)	597	WP_003095839.1	type 4a pilus biogenesis protein PilN	Machinery gene
P7I93_RS29865 (P7I93_29865)	pilM	6282736..6283788 (-)	1053	WP_034055307.1	type IV pilus assembly protein PilM	Machinery gene
P7I93_RS29870 (P7I93_29870)	-	6283893..6285197 (+)	1305	WP_010793081.1	IS1380-like element ISPa33 family transposase	-
P7I93_RS29875 (P7I93_29875)	-	6285550..6288021 (+)	2472	WP_023092706.1	penicillin-binding protein 1A	-

Sequence

Protein

• Length: 350 a.a.
• Molecular weight: 37586.95 Da
• Isoelectric Point: 4.5405

>NTDB_id=760204 P7I93_RS29865 WP_034055307.1 6282736..6283788(-) (pilM) [Pseudomonas aeruginosa strain 2022CK-00068]
MKKKANTLLGIDISSTSVKLLELSRSGGRYKVEAYAVEPLPPNAVVEKNIVELEGVGQALSRVLVKAKTNLKSAVVAVAG
SAVITKTIEMEAGLSEDELENQLKIEADQYIPYPLEEVAIDFEVQGLSARNPERVDVLLAACRKENVEVREAALALAGLT
AKVVDVEAYALERSYALLSSQLGADTDQLTVAVVDIGATMTTLSVLHNGRTIYTREQLFGGRQLTEEIQRRYGLSVEEAG
LAKKQGGLPDDYDSEVLRPFKDAVVQQVSRSLQFFFAAGQFNDVDYIVLAGGTASIQDLDRLIQQKIGTPTLVANPFADM
ALNGKVNAGALASDAPALMIACGLALRSFD

Nucleotide

• Length: 1053 bp

>NTDB_id=760204 P7I93_RS29865 WP_034055307.1 6282736..6283788(-) (pilM) [Pseudomonas aeruginosa strain 2022CK-00068]
ATAAAGAAGAAAGCGAACACCTTGCTGGGGATCGACATCAGCTCGACCTCGGTAAAGCTCCTTGAGTTGAGCCGCTCCGG
AGGCCGCTACAAAGTGGAGGCCTATGCCGTCGAGCCGCTTCCGCCGAATGCAGTCGTGGAAAAGAACATCGTCGAGCTGG
AAGGGGTCGGCCAGGCGTTGTCACGGGTGCTGGTCAAGGCGAAAACCAACCTGAAGTCGGCCGTGGTCGCCGTCGCCGGT
TCGGCGGTGATCACCAAGACCATCGAGATGGAGGCCGGGCTTTCCGAGGATGAACTGGAAAACCAGCTGAAGATCGAGGC
CGACCAGTACATCCCCTATCCGCTGGAGGAAGTCGCCATCGACTTCGAGGTCCAGGGACTCTCCGCGCGCAATCCCGAGC
GCGTGGACGTACTCCTGGCCGCCTGTCGGAAAGAGAACGTCGAGGTTCGCGAAGCGGCCCTGGCGCTGGCCGGCCTGACC
GCCAAGGTGGTCGACGTCGAGGCCTACGCGCTGGAGCGCTCCTATGCGCTGCTGAGCAGCCAGCTGGGTGCCGACACCGA
CCAGTTGACCGTGGCGGTAGTGGACATCGGCGCGACCATGACCACCCTGAGCGTCCTGCACAACGGACGCACCATCTATA
CCCGCGAACAGCTGTTCGGCGGTCGCCAGCTCACCGAGGAGATCCAGCGGCGTTACGGACTCTCGGTGGAGGAAGCCGGT
CTCGCCAAGAAGCAGGGCGGTCTTCCGGATGACTACGACAGCGAAGTTCTGCGTCCGTTCAAGGACGCCGTGGTGCAGCA
GGTTTCCCGCTCGCTGCAGTTCTTCTTCGCCGCCGGACAGTTCAACGACGTCGACTACATCGTCCTGGCGGGCGGTACGG
CGTCTATCCAGGATCTCGATCGGTTGATCCAGCAGAAGATCGGCACCCCGACCCTGGTCGCCAACCCGTTCGCCGACATG
GCGCTGAACGGCAAGGTGAATGCCGGCGCCCTGGCCAGCGACGCTCCGGCGTTGATGATCGCCTGCGGCCTGGCGTTGAG
GAGTTTCGACTGA

Domains

Predicted by InterProScan.

• Type IV pilus assembly protein PilM; (PilM_2) (10-349)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	55.84	100	0.56
comM	Acinetobacter nosocomialis M2	55.84	100	0.56
comM/pilM	Acinetobacter baylyi ADP1	51.862	99.714	0.517
pilM	Legionella pneumophila strain ERS1305867	46.286	100	0.463