Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   P7I93_RS29855 Genome accession   NZ_CP124658
Coordinates   6281520..6282143 (-) Length   207 a.a.
NCBI ID   WP_003103268.1    Uniprot ID   G3XD51
Organism   Pseudomonas aeruginosa strain 2022CK-00068     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6276520..6287143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I93_RS29835 (P7I93_29835) aroB 6277115..6278221 (-) 1107 WP_003107839.1 3-dehydroquinate synthase -
  P7I93_RS29840 (P7I93_29840) aroK 6278271..6278789 (-) 519 WP_003095833.1 shikimate kinase AroK -
  P7I93_RS29845 (P7I93_29845) pilQ 6278801..6280945 (-) 2145 WP_003095835.1 type 4a pilus secretin PilQ Machinery gene
  P7I93_RS29850 (P7I93_29850) comL/pilP 6280999..6281523 (-) 525 WP_003095836.1 type 4a pilus biogenesis lipoprotein PilP Machinery gene
  P7I93_RS29855 (P7I93_29855) pilO 6281520..6282143 (-) 624 WP_003103268.1 type 4a pilus biogenesis protein PilO Machinery gene
  P7I93_RS29860 (P7I93_29860) pilN 6282140..6282736 (-) 597 WP_003095839.1 type 4a pilus biogenesis protein PilN Machinery gene
  P7I93_RS29865 (P7I93_29865) pilM 6282736..6283788 (-) 1053 WP_034055307.1 type IV pilus assembly protein PilM Machinery gene
  P7I93_RS29870 (P7I93_29870) - 6283893..6285197 (+) 1305 WP_010793081.1 IS1380-like element ISPa33 family transposase -

Sequence

Protein


Download         Length: 207 a.a.        Molecular weight: 22819.26 Da        Isoelectric Point: 4.7787

>NTDB_id=760202 P7I93_RS29855 WP_003103268.1 6281520..6282143(-) (pilO) [Pseudomonas aeruginosa strain 2022CK-00068]
MSLASSLESLRKIDINDLDLNNIGSWPAAVKVIVCVLLTAAVLALGYNFHLSDMQAQLEQQAAEEETLKQQFSTKAFQAA
NLEAYKAQMKEMEESFGALLRQLPSDTEVPGLLEDITRTGLGSGLEFEEIKLLPEVAQQFYIELPIQISVVGGYHDLATF
VSGVSSLPRIVTLHDFEIKPVAPGSTSKLRMSILAKTYRYNDKGLKK

Nucleotide


Download         Length: 624 bp        

>NTDB_id=760202 P7I93_RS29855 WP_003103268.1 6281520..6282143(-) (pilO) [Pseudomonas aeruginosa strain 2022CK-00068]
ATGAGTCTGGCCAGTTCCCTGGAAAGTCTGCGCAAGATCGATATCAACGATCTCGACCTGAACAACATCGGTTCCTGGCC
GGCGGCGGTCAAGGTCATCGTCTGCGTGCTGCTGACCGCGGCGGTCCTGGCGCTGGGCTACAACTTCCATCTGAGTGACA
TGCAGGCTCAGCTCGAACAGCAGGCCGCGGAAGAGGAGACGCTCAAGCAGCAGTTCTCCACCAAGGCCTTCCAGGCCGCG
AACCTGGAAGCCTACAAGGCACAGATGAAGGAGATGGAAGAGTCCTTCGGCGCCTTGCTGCGGCAGTTGCCCAGCGACAC
CGAGGTACCCGGGCTGCTCGAGGACATCACTCGTACCGGCCTGGGCAGCGGCCTTGAGTTCGAGGAAATCAAGCTGCTTC
CCGAGGTTGCCCAGCAGTTCTACATCGAGCTGCCGATCCAGATCAGCGTGGTCGGCGGCTACCACGACCTGGCGACCTTC
GTCAGCGGCGTGTCCAGCCTGCCGCGGATCGTCACCCTGCATGACTTCGAGATCAAGCCGGTCGCGCCCGGCAGCACGTC
CAAGCTGCGCATGAGCATCCTGGCCAAGACCTATCGCTACAACGACAAGGGGCTGAAGAAATGA

Domains

Predicted by InterProScan.

Pilus assembly protein, PilO (PilO)

(58-202)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB G3XD51

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

46.114

93.237

0.43
  pilO Acinetobacter baumannii D1279779

43.979

92.271

0.406
  comO/pilO Acinetobacter baylyi ADP1

43.455

92.271

0.401