Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilA   Type   Machinery gene
Locus tag   P7I81_RS05790 Genome accession   NZ_CP124648
Coordinates   1219636..1220100 (-) Length   154 a.a.
NCBI ID   WP_124089607.1    Uniprot ID   -
Organism   Pseudomonas aeruginosa strain 2020CK-00220     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1214636..1225100
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  P7I81_RS05775 (P7I81_05775) nadC 1217156..1218004 (+) 849 WP_124089605.1 carboxylating nicotinate-nucleotide diphosphorylase -
  P7I81_RS05785 (P7I81_05785) - 1218185..1219570 (-) 1386 WP_074222679.1 O-antigen ligase family protein -
  P7I81_RS05790 (P7I81_05790) pilA 1219636..1220100 (-) 465 WP_124089607.1 pilin Machinery gene
  P7I81_RS05795 (P7I81_05795) pilB 1220331..1222031 (+) 1701 WP_003122078.1 type IV-A pilus assembly ATPase PilB Machinery gene
  P7I81_RS05800 (P7I81_05800) pilC 1222035..1223252 (+) 1218 WP_003107299.1 type II secretion system F family protein Machinery gene
  P7I81_RS05805 (P7I81_05805) pilD 1223253..1224125 (+) 873 WP_023084852.1 type IV prepilin peptidase/methyltransferase PilD Machinery gene
  P7I81_RS05810 (P7I81_05810) coaE 1224122..1224733 (+) 612 WP_003094654.1 dephospho-CoA kinase -
  P7I81_RS05815 (P7I81_05815) yacG 1224730..1224930 (+) 201 WP_003094656.1 DNA gyrase inhibitor YacG -

Sequence

Protein


Download         Length: 154 a.a.        Molecular weight: 16253.62 Da        Isoelectric Point: 9.0066

>NTDB_id=759433 P7I81_RS05790 WP_124089607.1 1219636..1220100(-) (pilA) [Pseudomonas aeruginosa strain 2020CK-00220]
MKAQKGFTLIELMIVVAIIGILAAIAIPQYQDYTARTQVTRAVSEVSALKTAAESAILEGKEIVSSTTPNNTQYDIGFTE
STLLDGSGKSQIKVTDNKDGTVEMVATLGKSSGSAIKGAVITVSRDAQGVWNCKISKTPTAWKPNYAPANCPKS

Nucleotide


Download         Length: 465 bp        

>NTDB_id=759433 P7I81_RS05790 WP_124089607.1 1219636..1220100(-) (pilA) [Pseudomonas aeruginosa strain 2020CK-00220]
ATGAAAGCTCAGAAGGGTTTTACTCTGATCGAACTGATGATCGTGGTTGCGATCATCGGCATCCTGGCCGCCATTGCCAT
CCCGCAATATCAGGACTACACCGCCCGTACCCAGGTGACCCGTGCCGTGAGTGAAGTCAGCGCGCTGAAGACCGCTGCGG
AGTCGGCGATTCTGGAAGGGAAGGAGATTGTTTCCAGCACGACTCCTAACAATACCCAGTATGACATTGGCTTCACCGAG
TCTACTTTGCTAGATGGTTCTGGTAAGAGTCAGATCAAGGTAACGGACAATAAAGATGGCACCGTTGAGATGGTCGCTAC
CTTGGGTAAATCTTCTGGTTCCGCCATCAAAGGGGCTGTCATCACTGTTTCGCGTGACGCTCAAGGAGTCTGGAACTGCA
AAATCTCCAAAACTCCTACAGCTTGGAAGCCCAACTACGCTCCGGCTAATTGCCCGAAATCCTAA

Domains

Predicted by InterProScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(1-26)

Pilin (bacterial filament) (Pilin)

(35-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilA Acinetobacter baumannii strain A118

44.079

98.701

0.435
  pilA Pseudomonas aeruginosa PAK

42.308

100

0.429
  pilA/pilAI Pseudomonas stutzeri DSM 10701

41.83

99.351

0.416
  pilA Vibrio parahaemolyticus RIMD 2210633

38.961

100

0.39
  pilA Vibrio cholerae strain A1552

36.364

100

0.364
  pilA Vibrio cholerae C6706

36.364

100

0.364
  pilA Vibrio cholerae O1 biovar El Tor strain E7946

36.364

100

0.364