Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KI243_RS02115 Genome accession   NZ_AP021904
Coordinates   474604..476682 (+) Length   692 a.a.
NCBI ID   WP_178990542.1    Uniprot ID   -
Organism   Pseudomonas sp. Ost2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 469604..481682
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI243_RS02095 (PHLH7_04070) pilM 471818..472882 (+) 1065 WP_017126915.1 pilus assembly protein PilM Machinery gene
  KI243_RS02100 (PHLH7_04080) - 472882..473448 (+) 567 WP_178990540.1 PilN domain-containing protein -
  KI243_RS02105 (PHLH7_04090) pilO 473445..474068 (+) 624 WP_017126913.1 type 4a pilus biogenesis protein PilO -
  KI243_RS02110 (PHLH7_04100) - 474065..474592 (+) 528 WP_042935612.1 pilus assembly protein PilP -
  KI243_RS02115 (PHLH7_04110) pilQ 474604..476682 (+) 2079 WP_178990542.1 type IV pilus secretin PilQ Machinery gene
  KI243_RS02120 (PHLH7_04120) aroK 476687..477205 (+) 519 WP_042935610.1 shikimate kinase AroK -
  KI243_RS02125 (PHLH7_04130) aroB 477355..478455 (+) 1101 WP_017126909.1 3-dehydroquinate synthase -
  KI243_RS02130 (PHLH7_04140) - 478466..480064 (+) 1599 WP_212810394.1 AAA family ATPase -

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74733.93 Da        Isoelectric Point: 5.4135

>NTDB_id=75704 KI243_RS02115 WP_178990542.1 474604..476682(+) (pilQ) [Pseudomonas sp. Ost2]
MNRIISTFGISLWMALVSPMVLAANLKTLDVAALPGDRVELRLAFDSAPAAPHGYTTDQPARIALDLTGVTSQLATRTRD
LGVGNARSVTVVEAKDRTRLIINLTKLVPYSTRIEGNNLFVVVGQGAAAAVPGQASAARPVAAAVAQPARAAPKPYVPAG
KAIRNIDFQRGEQGEGNVVIELSDPSISPDIQEKGGKISVEFPRTQLPDPLRVRLDVKDFATPVQFVNATASADRASISI
EPSGAFDYSTYQTDNKLTISVRPLTVDDLQRRNAERFVYSGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITL
RLQNVPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVT
SAEAKGDERGSITVDDRTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVGYDKQLGARWGGSISGGNWKGS
GGSPTLSKLTGQSASTPFVDLGATTATSGIGIGFVTDNTILDLELSAMEKTGNGEVVSQPKVVTSDKETAKILKGTEIPY
QESSSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYQNVLNNVPPIKKNEVNAKVLVNDGETVVIGGVFSN
TQSKTEEKVPFLGDVPYVGRLFRRDIVSEAKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=75704 KI243_RS02115 WP_178990542.1 474604..476682(+) (pilQ) [Pseudomonas sp. Ost2]
ATGAACAGGATTATTTCCACCTTCGGCATTTCGCTATGGATGGCGCTGGTGTCGCCGATGGTGCTGGCCGCCAACCTCAA
GACCCTCGACGTGGCGGCGCTGCCGGGAGATCGGGTTGAGCTCAGGCTCGCCTTCGACAGCGCCCCCGCCGCGCCCCATG
GCTATACCACCGATCAGCCGGCGCGGATTGCCCTGGACCTGACCGGGGTGACCAGCCAGTTGGCGACCAGGACCCGTGAC
CTGGGCGTGGGCAATGCACGCAGCGTGACCGTGGTGGAAGCCAAGGATCGTACCCGGCTGATCATCAACCTGACCAAGCT
GGTGCCCTACAGCACCCGTATCGAGGGTAACAACCTGTTCGTGGTGGTCGGCCAGGGCGCGGCAGCCGCCGTGCCGGGCC
AGGCCAGTGCGGCCCGGCCGGTGGCTGCCGCGGTGGCGCAACCCGCGCGTGCGGCGCCCAAGCCGTACGTACCGGCGGGC
AAGGCCATTCGCAATATCGACTTCCAGCGCGGCGAGCAGGGCGAAGGCAATGTGGTCATCGAGCTCTCCGACCCGTCCAT
CAGCCCGGATATCCAGGAAAAAGGCGGCAAGATCAGCGTGGAGTTCCCTCGCACCCAACTGCCCGACCCCCTGCGTGTGC
GCCTGGACGTGAAGGATTTCGCCACGCCGGTGCAATTCGTCAATGCCACGGCGTCCGCCGACCGGGCGAGCATTTCCATC
GAGCCGAGCGGGGCCTTCGACTACTCGACGTACCAGACCGACAACAAGCTGACCATCAGTGTCCGTCCGCTGACCGTCGA
CGACCTGCAGCGGCGTAATGCCGAGCGTTTTGTCTACAGCGGCGAAAAGCTCTCGCTGAACTTCCAGGACATCGATGTGC
GTTCGGTGCTGCAACTGATCGCCGACTTCACCAACCTGAACCTGGTGGCCAGCGACACGGTGCAGGGCGGGATTACCCTG
CGCCTGCAGAACGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGATCGGCAA
CGTCCTGCTGGTGGCGCCGGCGGACGAGATCGCGGCGCGTGAGCGTCAGGAGCTGGAGTCGCAGAAGCAGATCGCCGAAC
TGGCCCCGCTGCGCCGCGAGCTGCTGCAGGTCAACTACGCGAAGGCGGCGGACATCGCCAAGCTGTTCCAGTCGGTGACC
AGCGCCGAGGCCAAGGGTGACGAGCGGGGTTCGATCACCGTGGATGACCGCACCAACAACATCATTGCCTACCAGACCCA
GGACCGCCTCGACGAGCTGCGGCGGATCGTGGCGCAACTGGATATCCCGGTGCGCCAGGTGATGATCGAGGCGCGCATCG
TCGAAGCCAACGTCGGTTATGACAAGCAACTGGGCGCCCGTTGGGGTGGCAGCATTTCCGGCGGCAACTGGAAGGGCAGC
GGCGGTTCGCCGACCCTGTCGAAGCTGACCGGCCAGAGCGCCAGTACACCGTTCGTCGACCTGGGGGCCACCACGGCGAC
CTCCGGTATCGGCATCGGCTTCGTGACCGACAACACGATCCTCGACCTCGAACTGAGCGCGATGGAGAAGACCGGTAACG
GTGAAGTCGTGTCGCAGCCCAAGGTGGTCACCTCCGACAAGGAGACCGCGAAGATCCTCAAGGGCACGGAGATTCCGTAC
CAGGAGTCCAGCTCCAGCGGGGCCACCTCGGTGTCCTTCAAGGAAGCGTCGCTGTCCCTCGAGGTCACGCCGCAGATCAC
CCCGGACAACCGCATCATCATGGAAGTGAAGGTCACCAAGGATGAGCCGGACTACCAGAACGTGCTCAACAACGTACCGC
CGATCAAGAAGAACGAAGTCAACGCCAAGGTACTGGTCAACGACGGTGAAACCGTGGTGATCGGTGGCGTGTTCTCCAAT
ACTCAGAGCAAAACCGAAGAGAAGGTGCCATTTCTTGGCGATGTGCCGTATGTTGGCCGCCTTTTCCGCCGAGACATTGT
CTCGGAAGCAAAATCCGAGCTGCTGGTATTCCTGACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(381-446)

AMIN domain (AMIN)

(37-122)

Secretin and TonB N terminus short domain (STN)

(307-353)

Bacterial type II and III secretion system protein (Secretin)

(527-683)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

74.581

100

0.772
  comQ Acinetobacter baylyi ADP1

41.53

100

0.439
  pilQ Acinetobacter baumannii D1279779

38.8

100

0.421
  pilQ Acinetobacter baumannii strain A118

38.8

100

0.421
  pilQ Legionella pneumophila strain Lp02

38.496

97.977

0.377
  pilQ Legionella pneumophila strain ERS1305867

38.496

97.977

0.377

Multiple sequence alignment