Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KI243_RS02095 Genome accession   NZ_AP021904
Coordinates   471818..472882 (+) Length   354 a.a.
NCBI ID   WP_017126915.1    Uniprot ID   -
Organism   Pseudomonas sp. Ost2     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 466818..477882
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI243_RS02080 (PHLH7_04040) - 466846..467634 (+) 789 WP_212810393.1 thermonuclease family protein -
  KI243_RS02085 (PHLH7_04050) - 467818..469086 (+) 1269 WP_017126917.1 malic enzyme-like NAD(P)-binding protein -
  KI243_RS02090 (PHLH7_04060) - 469179..471614 (-) 2436 WP_178990538.1 penicillin-binding protein 1A -
  KI243_RS02095 (PHLH7_04070) pilM 471818..472882 (+) 1065 WP_017126915.1 pilus assembly protein PilM Machinery gene
  KI243_RS02100 (PHLH7_04080) - 472882..473448 (+) 567 WP_178990540.1 PilN domain-containing protein -
  KI243_RS02105 (PHLH7_04090) pilO 473445..474068 (+) 624 WP_017126913.1 type 4a pilus biogenesis protein PilO -
  KI243_RS02110 (PHLH7_04100) - 474065..474592 (+) 528 WP_042935612.1 pilus assembly protein PilP -
  KI243_RS02115 (PHLH7_04110) pilQ 474604..476682 (+) 2079 WP_178990542.1 type IV pilus secretin PilQ Machinery gene
  KI243_RS02120 (PHLH7_04120) aroK 476687..477205 (+) 519 WP_042935610.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38004.36 Da        Isoelectric Point: 4.6926

>NTDB_id=75703 KI243_RS02095 WP_017126915.1 471818..472882(+) (pilM) [Pseudomonas sp. Ost2]
MLGLFGKKAHSLLGIDISSTSVKLLELSRSGNRYRVESYAVEPLPPNAVVEKNIAELEGVGQALARVLLKAKTSARHVAV
AVAGSAVITKTIEMDAGLSDDDMESQLKIEADQYIPYPLEEVAIDFEVQGYSARNPERVEVLLAACRKENVEVREAALAL
AGLDAKVVDVEAYALERSFGLLAAQLASDHDRLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLSN
EEAGLAKKQGGLPDDYVAEVLQPFKDAVVQQVSRSLQFFFASGQYNDVDYILLAGGTASIAGLDHLIQQRLGTPTLVANP
FADMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=75703 KI243_RS02095 WP_017126915.1 471818..472882(+) (pilM) [Pseudomonas sp. Ost2]
GTGCTTGGACTCTTTGGCAAAAAAGCGCATTCGCTCCTGGGGATCGACATCAGTTCCACCTCGGTAAAACTCCTCGAATT
GAGCCGTTCAGGCAATCGATATCGGGTCGAGTCCTACGCCGTGGAACCGCTGCCGCCTAACGCCGTGGTCGAGAAGAACA
TCGCCGAGCTCGAAGGCGTCGGGCAGGCCCTGGCACGGGTGCTGCTCAAGGCCAAGACCAGCGCGCGCCACGTCGCGGTC
GCCGTGGCCGGTTCCGCGGTGATCACCAAGACCATCGAGATGGATGCCGGGCTTTCCGATGACGACATGGAAAGCCAACT
GAAGATAGAAGCCGACCAGTACATTCCCTATCCCCTCGAAGAAGTGGCCATCGACTTTGAAGTCCAGGGTTATTCGGCGC
GCAATCCCGAGCGCGTCGAAGTACTGCTGGCCGCCTGTCGGAAAGAAAACGTCGAGGTGCGCGAGGCGGCCCTGGCCCTG
GCCGGGCTCGATGCCAAGGTGGTGGATGTCGAGGCCTATGCCCTGGAGCGCTCCTTTGGCCTGCTCGCGGCACAGCTGGC
CAGCGATCACGACCGCTTGACGGTGGCGGTGGTCGACATCGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCC
GGATCATCTACACCCGCGAACAATTGTTCGGCGGTCGCCAGCTCACCGAGGAAATCCAGCGGCGCTACGGCCTGTCCAAC
GAAGAGGCGGGCCTGGCGAAGAAGCAGGGCGGCCTGCCCGACGACTATGTCGCCGAGGTCCTGCAGCCCTTCAAGGATGC
CGTGGTCCAGCAGGTGTCGCGTTCCCTGCAGTTCTTCTTCGCCTCGGGCCAGTACAACGATGTCGACTACATCCTGCTTG
CCGGTGGCACCGCCTCGATTGCCGGCCTCGATCACCTGATCCAGCAGCGGCTCGGTACGCCGACCCTGGTGGCCAACCCT
TTCGCCGACATGGCGTTGAGCAGCAAGGTCAATGCCGGGGCCCTGGCCAGTGACGCGCCGGCCCTGATGATCGCCTGCGG
TCTGGCGTTGAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

57.91

100

0.579
  comM Acinetobacter nosocomialis M2

57.627

100

0.576
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

48.169

100

0.483

Multiple sequence alignment