Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KI231_RS02115 Genome accession   NZ_AP021903
Coordinates   489295..491376 (+) Length   693 a.a.
NCBI ID   WP_213027296.1    Uniprot ID   -
Organism   Pseudomonas sp. Seg1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 484295..496376
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI231_RS02095 (PHLH6_04170) pilM 486507..487571 (+) 1065 WP_213027293.1 pilus assembly protein PilM Machinery gene
  KI231_RS02100 (PHLH6_04180) - 487571..488137 (+) 567 WP_213027294.1 PilN domain-containing protein -
  KI231_RS02105 (PHLH6_04190) pilO 488134..488757 (+) 624 WP_103305450.1 type 4a pilus biogenesis protein PilO -
  KI231_RS02110 (PHLH6_04200) - 488754..489281 (+) 528 WP_213027295.1 pilus assembly protein PilP -
  KI231_RS02115 (PHLH6_04210) pilQ 489295..491376 (+) 2082 WP_213027296.1 type IV pilus secretin PilQ Machinery gene
  KI231_RS02120 (PHLH6_04220) aroK 491381..491899 (+) 519 WP_045122260.1 shikimate kinase AroK -
  KI231_RS02125 (PHLH6_04230) aroB 492053..493153 (+) 1101 WP_213027297.1 3-dehydroquinate synthase -
  KI231_RS02130 (PHLH6_04240) - 493164..494756 (+) 1593 WP_103305446.1 AAA family ATPase -

Sequence

Protein


Download         Length: 693 a.a.        Molecular weight: 75049.50 Da        Isoelectric Point: 5.5676

>NTDB_id=75675 KI231_RS02115 WP_213027296.1 489295..491376(+) (pilQ) [Pseudomonas sp. Seg1]
MNRIFSTLGFSLWIALMSPMVLAANLKTLDVAALPGDRVELKLSFDGPPPQPKGYTTDSPARIALDLLGVASQLASKNLD
LGSGNARTATVVEAKDRTRLIVNLTQPAPYNTRVEGNNLFVVVGQGAPASAPRPAAVAPVRATAAGAPAKAFVPKTKAIR
GVDFQRGTAGEGNVVIDLSDPTIAPDIQEHDGKIILSFARTQLPDKLRVRLDVKDFATPVQFVNAAATGDRAIITVEPSG
TFDYSTYQTDNKLTVSIRPMTVDDLQKRNADRQAYVGDKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIAELAPLRRELLQVNYAKAADIAKLFQSVTSAEA
KIDERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVS
NGSSTTIGTPGSTSTNSPFVDMGTVNNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTEIP
YQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGGVFS
NTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2082 bp        

>NTDB_id=75675 KI231_RS02115 WP_213027296.1 489295..491376(+) (pilQ) [Pseudomonas sp. Seg1]
ATGAACAGGATTTTCTCCACCCTCGGTTTTTCGCTATGGATAGCGCTGATGTCGCCGATGGTACTCGCGGCCAATCTGAA
GACGCTGGACGTGGCGGCGTTGCCGGGTGACCGCGTCGAACTGAAGCTGTCGTTCGATGGCCCGCCGCCGCAGCCCAAGG
GCTACACCACCGATTCACCGGCGCGAATCGCGCTGGATCTGCTCGGGGTCGCCAGTCAGTTGGCCAGCAAGAACCTCGAT
CTGGGCAGTGGCAACGCACGCACGGCCACGGTGGTTGAGGCCAAGGATCGCACGCGGCTGATCGTCAATCTTACCCAGCC
AGCGCCTTACAACACGCGAGTCGAAGGCAACAATCTGTTCGTGGTGGTCGGTCAGGGGGCGCCGGCAAGCGCTCCCCGTC
CCGCCGCTGTTGCTCCAGTGCGCGCGACTGCGGCAGGGGCTCCCGCCAAAGCGTTCGTGCCGAAAACCAAAGCCATTCGC
GGCGTGGACTTTCAGCGCGGCACCGCAGGCGAAGGCAATGTGGTCATCGACCTGTCCGACCCGACCATCGCTCCGGATAT
TCAGGAGCATGACGGCAAGATCATCCTCAGCTTCGCCCGCACGCAATTGCCCGACAAGCTGCGGGTACGCCTCGACGTCA
AGGATTTCGCCACGCCCGTGCAGTTCGTCAACGCTGCTGCAACAGGCGATCGCGCGATCATTACCGTTGAGCCCAGCGGT
ACCTTCGACTATTCCACCTACCAGACCGACAACAAGTTGACCGTCAGCATCCGTCCGATGACCGTCGACGATCTGCAAAA
GCGCAACGCCGATCGTCAGGCCTACGTCGGCGACAAGCTGTCGCTGAATTTTCAGGACATCGACGTGCGCTCGGTGCTGC
AACTGATCGCCGACTTCACCAACCTCAATCTGGTGGCCAGCGATACGGTGCAGGGCGGCATCACTCTGCGTCTGCAAAAT
GTGCCATGGGATCAGGCGCTGGATCTGGTGCTGAAAACCAAGGGGCTGGACAAGCGCAAGATCGGCAATGTGCTGCTGGT
CGCCCCGGCAGACGAAATCGCCGCCCGCGAGCGCCAGGAACTGGAGTCGCAAAAACAGATCGCCGAACTGGCGCCGCTGC
GCCGTGAACTGCTGCAGGTGAACTACGCCAAGGCTGCCGACATCGCCAAGCTGTTCCAGTCGGTGACCAGCGCCGAAGCG
AAAATCGACGAGCGCGGCTCGATCACCGTCGACGAGCGAACCAACAACATCATTGCCTACCAGACTCAGGACCGGCTCGA
CGAACTACGGCGAATCGTGGCGCAACTGGATATTCCCGTGCGTCAGGTGATGATCGAGGCGCGAATCGTCGAAGCCAACG
TCGATTACGACAAGAGCCTGGGTGTGCGCTGGGGTGGGTCGATCCAGAACAAGGGCAACTGGAACACTTCCGGCGTCAGT
AACGGCTCGTCGACCACCATCGGCACCCCGGGCAGCACCAGCACCAATTCGCCGTTCGTCGACATGGGCACCGTCAACAA
TACTTCGGGGATTGGCATTGCCTTCATCACCGACAACGTTTTGCTCGATCTTGAGCTGACTGCCATGGAGAAAACCGGCA
ATGGCGAAATCGTCTCGCAGCCCAAGGTGGTGACTTCCGACAAGGAGACCGCGAAGATCCTCAAGGGCACCGAAATTCCT
TATCAGGAGGCCAGCTCAAGTGGCGCCACTTCGGTGTCGTTCAAGGAAGCTTCGCTGTCGCTGGAGGTGACGCCGCAGAT
CACCCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGACGAGCCTGATTATCTGAACAAAGTGCAGGATGTAC
CGCCGATCAAAAAGAACGAGGTCAACGCCAAAGTGCTGGTAAACGACGGCGAGACCATCGTGATTGGGGGCGTTTTCTCA
AATACTCAAAGCAAGGTCGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGTGATGT
GGTTTCGGAGAAAAAATCCGAGCTGCTGGTATTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTT
GA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(303-349)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)

Bacterial type II and III secretion system protein (Secretin)

(528-684)

AMIN domain (AMIN)

(37-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.943

100

0.755
  comQ Acinetobacter baylyi ADP1

41.499

100

0.416
  pilQ Acinetobacter baumannii D1279779

37.871

100

0.405
  pilQ Acinetobacter baumannii strain A118

37.871

100

0.405
  pilQ Legionella pneumophila strain Lp02

37.758

97.835

0.369
  pilQ Legionella pneumophila strain ERS1305867

37.722

97.547

0.368

Multiple sequence alignment