Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   KI231_RS02095 Genome accession   NZ_AP021903
Coordinates   486507..487571 (+) Length   354 a.a.
NCBI ID   WP_213027293.1    Uniprot ID   -
Organism   Pseudomonas sp. Seg1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 481507..492571
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI231_RS02085 (PHLH6_04150) - 482311..483579 (+) 1269 WP_103305454.1 malic enzyme-like NAD(P)-binding protein -
  KI231_RS02090 (PHLH6_04160) - 483844..486291 (-) 2448 WP_103305453.1 penicillin-binding protein 1A -
  KI231_RS02095 (PHLH6_04170) pilM 486507..487571 (+) 1065 WP_213027293.1 pilus assembly protein PilM Machinery gene
  KI231_RS02100 (PHLH6_04180) - 487571..488137 (+) 567 WP_213027294.1 PilN domain-containing protein -
  KI231_RS02105 (PHLH6_04190) pilO 488134..488757 (+) 624 WP_103305450.1 type 4a pilus biogenesis protein PilO -
  KI231_RS02110 (PHLH6_04200) - 488754..489281 (+) 528 WP_213027295.1 pilus assembly protein PilP -
  KI231_RS02115 (PHLH6_04210) pilQ 489295..491376 (+) 2082 WP_213027296.1 type IV pilus secretin PilQ Machinery gene
  KI231_RS02120 (PHLH6_04220) aroK 491381..491899 (+) 519 WP_045122260.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38041.58 Da        Isoelectric Point: 4.8018

>NTDB_id=75674 KI231_RS02095 WP_213027293.1 486507..487571(+) (pilM) [Pseudomonas sp. Seg1]
MLGLFNKKTNTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPANAVVEKNIAELEGVGQALTRVLVKARTGVKSVAV
AVAGSAVITKIIEMDAGLSDDELENQLKIEADQYIPYPLDEVAIDFEVQGVSARNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSFGLLATQLAASQQRLTVAVVDIGATMTTLSVLHNGKIIYTREQLFGGRQLTEEIQRRYGLTI
EQAGLAKKQGGLPDDYVSDVLQPFREALVQQVSRSLQFFFASGQYNAVDHILLAGGTASVPGLDRLIEQRLDTPTQVANP
FSAMALSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=75674 KI231_RS02095 WP_213027293.1 486507..487571(+) (pilM) [Pseudomonas sp. Seg1]
GTGCTGGGACTCTTCAATAAAAAGACCAATACGTTACTGGGGATCGACATCAGCTCCACGTCAGTGAAGCTGCTGGAACT
GAGCCGTCAGGGTGATCGCTACCGGGTCGAGGCGTATGCGGTGGAGCCATTGCCAGCGAACGCCGTGGTCGAAAAGAACA
TCGCCGAACTCGAAGGCGTTGGCCAGGCTCTGACCCGGGTGCTGGTCAAGGCACGCACCGGGGTCAAGAGCGTCGCGGTG
GCGGTGGCCGGTTCTGCCGTGATCACCAAGATCATCGAAATGGACGCCGGGCTGTCCGACGACGAGCTGGAAAATCAGCT
CAAGATCGAAGCCGACCAATACATTCCCTATCCGCTGGACGAGGTCGCCATCGACTTTGAAGTCCAGGGCGTTTCTGCGC
GCAACCCCGAGCGAGTCAACGTACTGCTCGCCGCCTGCCGCAAGGAAAACGTCGAAGTCCGTGAAGCGGCGCTGGCACTC
GCCGGGCTGACCGCGCGCGTGGTCGATGTCGAGGCTTATGCGCTGGAGCGCTCGTTCGGCTTGCTCGCAACGCAACTGGC
CGCGTCGCAACAGCGCCTGACGGTGGCGGTGGTCGACATAGGCGCGACCATGACCACCCTCAGCGTCCTGCACAACGGCA
AGATCATCTACACCCGCGAGCAGTTGTTCGGCGGCCGGCAACTGACCGAGGAAATCCAGCGTCGTTATGGCCTGACAATC
GAGCAGGCGGGGCTGGCGAAAAAGCAGGGCGGCCTGCCTGACGACTACGTCAGCGATGTGCTGCAGCCGTTTCGCGAAGC
ACTGGTGCAACAGGTGTCGCGTTCATTGCAATTCTTCTTCGCTTCCGGGCAGTACAACGCGGTCGATCACATTCTGCTCG
CCGGTGGCACGGCGTCGGTGCCGGGGCTGGATCGCTTGATCGAGCAGCGCCTGGACACGCCGACGCAAGTGGCCAATCCA
TTTTCCGCCATGGCCTTGAGCAGCAAGGTCAACGCCGGAGCCCTGGCCAGTGACGCGCCGGCCCTGATGATTGCCTGCGG
GCTCGCGCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

56.215

100

0.562
  comM Acinetobacter nosocomialis M2

56.215

100

0.562
  comM Acinetobacter baylyi ADP1

55.085

100

0.551
  pilM Legionella pneumophila strain ERS1305867

48.023

100

0.48

Multiple sequence alignment