Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KI241_RS02005 Genome accession   NZ_AP021902
Coordinates   464990..467086 (+) Length   698 a.a.
NCBI ID   WP_042731986.1    Uniprot ID   A0A0D0PEH2
Organism   Pseudomonas sp. Cab53     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 459990..472086
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI241_RS01985 (PHLH5_03950) comM 462201..463265 (+) 1065 WP_047736134.1 pilus assembly protein PilM Machinery gene
  KI241_RS01990 (PHLH5_03960) - 463265..463831 (+) 567 WP_042731989.1 PilN domain-containing protein -
  KI241_RS01995 (PHLH5_03970) pilO 463828..464451 (+) 624 WP_042731988.1 type 4a pilus biogenesis protein PilO -
  KI241_RS02000 (PHLH5_03980) - 464448..464993 (+) 546 WP_047736133.1 pilus assembly protein PilP -
  KI241_RS02005 (PHLH5_03990) pilQ 464990..467086 (+) 2097 WP_042731986.1 type IV pilus secretin PilQ Machinery gene
  KI241_RS02010 (PHLH5_04000) aroK 467091..467609 (+) 519 WP_042731985.1 shikimate kinase AroK -
  KI241_RS02015 (PHLH5_04010) aroB 467754..468854 (+) 1101 WP_042731984.1 3-dehydroquinate synthase -
  KI241_RS02020 (PHLH5_04020) - 468865..470442 (+) 1578 WP_212803300.1 AAA family ATPase -

Sequence

Protein


Download         Length: 698 a.a.        Molecular weight: 75109.37 Da        Isoelectric Point: 5.4030

>NTDB_id=75646 KI241_RS02005 WP_042731986.1 464990..467086(+) (pilQ) [Pseudomonas sp. Cab53]
MNRIFSAFGVSLGIALLSPMVHGAGLKTLDVAALPGDRVELKLSFDGPPPTPRGYTTEQPARIALDLPGVTNQLASKSHD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYNTRIEGNNLFVVVGKGAVPAITPATAPAPAAAAPRPALASATPVKARAPA
GKAIRGVDFQRGTQGEGNVVIDLSDPAIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGTDRATIS
IEPSGTFDYSTYQTDNKLTVSVRPMTVEDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGIT
LRLQNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSV
TSAEAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKNLGVRWGGSIQNQGNWN
SSGVTSGTSTTIGTPGSTGTNQPFVDMGVSGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILK
GTEIPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQEVPPIKKNEVNAKVLVNDGETIVI
GGVFSNTQNKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2097 bp        

>NTDB_id=75646 KI241_RS02005 WP_042731986.1 464990..467086(+) (pilQ) [Pseudomonas sp. Cab53]
ATGAACAGGATTTTTTCAGCCTTTGGTGTTTCGCTAGGGATAGCGCTCTTATCACCGATGGTTCATGGGGCCGGCCTGAA
GACCCTGGACGTGGCGGCGCTGCCGGGCGACCGCGTCGAATTGAAACTCTCCTTCGACGGGCCGCCGCCGACGCCGCGGG
GCTACACCACCGAACAGCCGGCGCGGATCGCCCTGGATCTGCCGGGCGTCACCAATCAACTGGCGAGCAAGAGCCATGAC
CTGGGCGGCGGCAATGCCCGCAGCGCCACCGTGGTCGAAGCAGGGGATCGCACCCGGCTGATCATAGGGCTGACTCAGTT
GGCGCCGTATAACACCCGCATCGAGGGTAACAACCTGTTCGTGGTGGTGGGCAAGGGCGCGGTTCCTGCGATAACCCCTG
CGACAGCCCCCGCGCCGGCCGCCGCGGCGCCGCGCCCGGCGCTGGCCAGTGCGACTCCGGTGAAGGCCCGCGCGCCGGCG
GGCAAGGCGATTCGCGGGGTGGACTTCCAGCGTGGCACCCAGGGCGAGGGCAATGTCGTGATCGACTTGTCCGACCCCGC
CATCGCGCCGGATATCCAGGAGCGTGAAGGCAAGATCATCCTCAATTTCGCCCGCACGCAGCTGCCCGAACCCCTGCGGG
TGCGCCTGGATGTGAAGGATTTCGCCACCCCGGTGCAGTTCGTCAATGCCAGTGCCGGCACCGACCGGGCGACGATCAGC
ATCGAGCCCAGTGGCACCTTCGATTATTCGACCTACCAGACCGACAACAAGCTCACCGTCAGCGTCCGGCCCATGACTGT
CGAGGACTTGCAGAAGCGCAATGCCGATCGCGCCGCCTACAGTGGTGAAAAGCTTTCCCTCAACTTCCAGGACATCGAGG
TGCGCTCGGTCCTGCAACTGATTGCCGATTTCACCAACCTGAACCTGGTGGCGAGCGACACGGTGCAGGGGGGCATCACC
CTGCGGTTGCAGAATGTGCCGTGGGACCAGGCGCTGGACCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAAGGTCGG
CAACGTGCTGCTGGTGGCGCCGGCCGACGAGATTGCGGCGCGTGAGCGCCAGGAGCTCGAATCCCAGAAGCAGATTGCCG
ACCTGGCGCCGCTGCGCCGGGAGCTGCTGCAGGTCAACTATGCCAAGGCCGCGGACATCGCCAAGCTGTTCCAGTCGGTG
ACCAGCGCCGAGGCCAAGGCCGATGAGCGTGGCACGATCACGGTCGATGAGCGCACCAACAACATCATTGCCTACCAGAC
CCAGGACCGGCTCGACGAGTTGCGTCGGATCGTCGCGCAGTTGGATATCCCGGTGCGTCAGGTAATGATCGAGGCCCGGA
TCGTCGAGGCCAACGTCGACTACGACAAGAACCTGGGCGTTCGCTGGGGCGGTTCGATACAGAACCAGGGCAACTGGAAC
TCTTCCGGGGTCACGTCCGGGACTTCCACGACCATCGGCACGCCGGGGAGTACGGGCACCAACCAGCCGTTCGTCGACAT
GGGTGTGTCAGGCAACACCTCGGGGATCGGCATTGCGTTCATCACCGACAACGTCCTGCTGGACCTGGAACTGACGGCGA
TGGAGAAAACCGGCAACGGCGAAATCGTTTCCCAGCCCAAGGTCGTGACCTCCGACAAGGAGACGGCGAAGATCCTCAAG
GGCACGGAAATTCCCTACCAGGAAGCCAGCTCCAGTGGTGCTACCTCGGTGTCGTTCAAGGAGGCCTCGCTGTCGCTGGA
AGTCACGCCGCAGATCACGCCGGACAACCGCATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCTGATTACCTGAACA
AGGTGCAGGAGGTGCCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTGCTGGTCAACGACGGCGAGACCATTGTGATT
GGCGGGGTTTTCTCGAATACTCAGAACAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCT
TTTCCGGCGTGATGTGGTCTCGGAGAAAAAATCTGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAACAACCAGGCGA
TTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(37-121)

Bacterial type II and III secretion system protein (Secretin)

(533-689)

Secretin and TonB N terminus short domain (STN)

(308-354)

Bacterial type II/III secretion system short domain (Secretin_N)

(382-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0D0PEH2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.145

100

0.742
  comQ Acinetobacter baylyi ADP1

42.06

100

0.421
  pilQ Acinetobacter baumannii strain A118

38.349

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.349

100

0.413
  pilQ Legionella pneumophila strain ERS1305867

38.382

97.421

0.374
  pilQ Legionella pneumophila strain Lp02

38.382

97.421

0.374

Multiple sequence alignment