Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KI237_RS02555 Genome accession   NZ_AP021901
Coordinates   584232..586319 (+) Length   695 a.a.
NCBI ID   WP_212798668.1    Uniprot ID   -
Organism   Pseudomonas sp. St316     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 579232..591319
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI237_RS02535 (PHLH4_05030) comM 581444..582508 (+) 1065 WP_212798664.1 pilus assembly protein PilM Machinery gene
  KI237_RS02540 (PHLH4_05040) - 582508..583074 (+) 567 WP_212798665.1 PilN domain-containing protein -
  KI237_RS02545 (PHLH4_05050) pilO 583071..583694 (+) 624 WP_212798666.1 type 4a pilus biogenesis protein PilO -
  KI237_RS02550 (PHLH4_05060) - 583691..584218 (+) 528 WP_212798667.1 pilus assembly protein PilP -
  KI237_RS02555 (PHLH4_05070) pilQ 584232..586319 (+) 2088 WP_212798668.1 type IV pilus secretin PilQ Machinery gene
  KI237_RS02560 (PHLH4_05080) aroK 586324..586842 (+) 519 WP_212798669.1 shikimate kinase AroK -
  KI237_RS02565 (PHLH4_05090) aroB 586991..588091 (+) 1101 WP_212798670.1 3-dehydroquinate synthase -
  KI237_RS02570 (PHLH4_05100) - 588101..589681 (+) 1581 WP_212798671.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 75061.09 Da        Isoelectric Point: 5.5610

>NTDB_id=75618 KI237_RS02555 WP_212798668.1 584232..586319(+) (pilQ) [Pseudomonas sp. St316]
MNRFFSALGISLWIALLSPMVHGASLKTLDVAALPGDRVELKLTFDGPPPTPRGYTTEQPARIALDLPGVTNQLSSKRRD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRVEGNNLFVVVGKGAVSTSTAPKSSAQAPRPAVVSAAPARASAPVGKA
IRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEHEGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEP
SGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKATDIAKLFQSVTSA
EAKADERGSITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWNSSG
VTTGNSTTVGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVNDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=75618 KI237_RS02555 WP_212798668.1 584232..586319(+) (pilQ) [Pseudomonas sp. St316]
ATGAACAGGTTTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGTTGTTGTCGCCGATGGTTCATGGGGCCAGCCTGAA
GACGTTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAGCTCACCTTCGACGGGCCACCGCCGACACCCCGGG
GCTACACCACCGAGCAACCGGCGAGGATTGCACTGGACCTGCCGGGCGTGACCAATCAGCTTTCCAGCAAACGCCGCGAC
CTGGGCGGGGGCAATGCACGCAGCGCTACCGTGGTCGAGGCTGGGGATCGTACCCGGTTGATCATCGGCCTTACCCAACT
GGCGCCGTATGACACCCGGGTCGAAGGTAACAATCTGTTCGTGGTGGTTGGCAAGGGCGCCGTCTCGACGAGTACTGCCC
CGAAGAGCTCCGCCCAGGCCCCGCGCCCGGCGGTTGTCAGCGCTGCACCTGCGAGGGCCAGTGCGCCAGTGGGCAAGGCG
ATTCGTGGCGTGGATTTCCAGCGCGGGACCCAGGGCGAAGGCAATGTGGTGATCGACCTGTCTGATCCGTCCATTGCCCC
GGACATCCAGGAGCATGAAGGCAAGATCATCCTCAACTTCGCCAGGACCCAGTTGCCCGAGCCGCTGCGGGTGCGCCTGG
ACGTCAAGGACTTCGCCACCCCGGTGCAATTCGTCAATGCCAGCGCCGGCAGCGACCGGGCGACCATCAGCATCGAACCC
AGCGGCACCTTCGACTACTCGACCTACCAGACCGACAACAAACTCACCGTCAGCGTCCGGCCCATGACCGTCGACGACCT
GCAAAAACGTAACGCCGACCGCGCGGCCTACAGTGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAGGTGCGTTCGG
TCCTGCAACTGATTGCCGATTTTACCAACCTTAACCTCGTCGCCAGCGACACGGTGCAGGGTGGCATCACCCTGCGGTTG
CAGAATGTGCCCTGGGACCAGGCGCTCGACCTGGTGCTCAAGACCAAGGGGTTGGACAAGCGCAAGGTCGGCAACGTGCT
GTTGGTGGCTCCGGCCGACGAAATTGCCGCCCGTGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATTGCCGACCTGGCGC
CGCTGCGGCGTGAACTGTTGCAGGTCAACTATGCGAAGGCGACGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCC
GAGGCGAAAGCCGATGAGCGCGGCTCGATCACGGTCGATGAACGCACCAACAACATCATTGCCTACCAGACCCAGGACCG
CCTCGACGAACTGCGCCGGATCGTGGCGCAACTGGATATTCCGGTACGCCAGGTGATGATCGAGGCGCGGATTGTCGAGG
CTAACGTCGACTACGACAAAAGCCTGGGCGTGCGCTGGGGCGGCTCGGTTCAAAACAAGGGCAACTGGAACTCCTCGGGG
GTCACCACTGGCAATTCCACCACCGTTGGCACGCCGGGTAGTACCAGCACCAACCAGCCGTTTGTCGACCTGGGCGCCTC
GGGCAATACCTCGGGCATCGGCATTGCCTTCATCACCGACAACGTCTTGCTGGACCTTGAGCTGACGGCCATGGAGAAAA
CCGGCAACGGGGAGATCGTCTCCCAGCCCAAGGTGGTGACGTCCGACAAGGAGACGGCAAAGATCCTCAAGGGCACGGAA
ATCCCTTATCAGGAAGCCAGCTCCAGCGGCGCTACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTTGAGGTGACGCC
GCAGATCACTCCAGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGAATG
ATGTACCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAATGATGGCGAGACCATTGTGATTGGCGGGGTT
TTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTTTCGGAGAAAAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(305-351)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

Bacterial type II and III secretion system protein (Secretin)

(530-686)

AMIN domain (AMIN)

(38-122)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.324

100

0.755
  comQ Acinetobacter baylyi ADP1

41.151

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.389

100

0.412
  pilQ Acinetobacter baumannii strain A118

38.389

100

0.412
  pilQ Legionella pneumophila strain Lp02

38.676

97.842

0.378
  pilQ Legionella pneumophila strain ERS1305867

38.439

97.698

0.376

Multiple sequence alignment