Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   KI237_RS02535 Genome accession   NZ_AP021901
Coordinates   581444..582508 (+) Length   354 a.a.
NCBI ID   WP_212798664.1    Uniprot ID   -
Organism   Pseudomonas sp. St316     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 576444..587508
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI237_RS02525 (PHLH4_05010) - 577274..578542 (+) 1269 WP_212798663.1 malic enzyme-like NAD(P)-binding protein -
  KI237_RS02530 (PHLH4_05020) - 578792..581236 (-) 2445 WP_212800538.1 penicillin-binding protein 1A -
  KI237_RS02535 (PHLH4_05030) comM 581444..582508 (+) 1065 WP_212798664.1 pilus assembly protein PilM Machinery gene
  KI237_RS02540 (PHLH4_05040) - 582508..583074 (+) 567 WP_212798665.1 PilN domain-containing protein -
  KI237_RS02545 (PHLH4_05050) pilO 583071..583694 (+) 624 WP_212798666.1 type 4a pilus biogenesis protein PilO -
  KI237_RS02550 (PHLH4_05060) - 583691..584218 (+) 528 WP_212798667.1 pilus assembly protein PilP -
  KI237_RS02555 (PHLH4_05070) pilQ 584232..586319 (+) 2088 WP_212798668.1 type IV pilus secretin PilQ Machinery gene
  KI237_RS02560 (PHLH4_05080) aroK 586324..586842 (+) 519 WP_212798669.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38166.75 Da        Isoelectric Point: 4.9155

>NTDB_id=75617 KI237_RS02535 WP_212798664.1 581444..582508(+) (comM) [Pseudomonas sp. St316]
MLRLFNKKAHALLGIDISSTSVKLLELSRQGERYRVESYAVEPLPANAVVEKNIAELEGVGQALSRLLAKARTPLRSVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVMGVSPRSNERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVIDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGLAKKQGGLPDDYPSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALGSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=75617 KI237_RS02535 WP_212798664.1 581444..582508(+) (comM) [Pseudomonas sp. St316]
GTGCTACGACTCTTCAATAAAAAAGCCCATGCGCTTCTGGGGATCGACATCAGTTCGACGTCGGTGAAGCTGCTTGAGTT
GAGCCGCCAGGGCGAGCGATACCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCAGCCAACGCCGTGGTCGAGAAGAACA
TCGCCGAGCTCGAAGGGGTGGGCCAGGCATTGTCGCGCCTGCTGGCCAAGGCCAGGACGCCCTTGCGGAGCGTGGCGGTG
GCCGTGGCGGGTTCGGCGGTGATCACCAAGACCATCGAGATGGACGCCGGGCTGTCCGACGATGAGATGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATCCCCTATCCCCTGGATGAGGTGGCCATTGATTTCGAAGTGATGGGTGTTTCGCCGC
GCAGTAACGAGCGCGTCGAAGTGTTGCTGGCGGCGTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCGTTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGATGTAGAGGCTTATGCGCTGGAACGTGCTTTCGGCCTGCTCGCCACGCAACTGGC
TGCTTCCCAGGAACGGCTGACCGTGGCGGTCATCGACATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAGCAATTGTTCGGCGGTCGACAACTGACCGAGGAGATCCAGCGTCGCTATGGCCTGACGCCC
GAGCAGGCCGGCCTGGCCAAGAAGCAGGGTGGGTTGCCGGACGATTATCCCAGTGAAGTGCTGCAGCCTTTTCGCGAGGC
CCTGGTACAGCAGGTCTCGCGGTCCTTGCAGTTCTTCTTCGCCTCGGGCCAGTACAGTGCGGTGGACCACATTCTGCTGG
CCGGAGGCACGGCTTCGGTCGCCGGCCTGGATCGGCTGATCGAGCAGCGCCTGGGCACGCCGACCCAGGTGGCAAACCCG
TTTACCAATATGGCCCTGGGCAGCAAGGTCAATGCCGGGGCGCTGGCCAGTGATGCGCCAGCGCTGATGATTGCCTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

48.023

100

0.48

Multiple sequence alignment