Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   BBP27_RS07290 Genome accession   NZ_CP110721
Coordinates   1553539..1555218 (+) Length   559 a.a.
NCBI ID   WP_188212066.1    Uniprot ID   -
Organism   Neisseria sp. RH3002v2g     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1548539..1560218
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BBP27_RS07280 - 1551311..1552069 (+) 759 WP_188212064.1 nucleotidyltransferase domain-containing protein -
  BBP27_RS07285 - 1552084..1553145 (+) 1062 WP_188212065.1 nucleotidyltransferase domain-containing protein -
  BBP27_RS07290 pilF 1553539..1555218 (+) 1680 WP_188212066.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BBP27_RS07295 pilG 1555298..1556539 (+) 1242 WP_070615539.1 type II secretion system F family protein Machinery gene
  BBP27_RS07300 pilD 1556541..1557428 (+) 888 WP_223168952.1 A24 family peptidase Machinery gene
  BBP27_RS07305 coaE 1557428..1558048 (+) 621 WP_188212067.1 dephospho-CoA kinase -
  BBP27_RS07310 yacG 1558045..1558227 (+) 183 WP_003682475.1 DNA gyrase inhibitor YacG -
  BBP27_RS07315 glyQ 1558311..1559210 (+) 900 WP_003685919.1 glycine--tRNA ligase subunit alpha -
  BBP27_RS07320 lspA 1559359..1559865 (+) 507 WP_188212068.1 signal peptidase II -

Sequence

Protein


Download         Length: 559 a.a.        Molecular weight: 62267.47 Da        Isoelectric Point: 5.8686

>NTDB_id=756008 BBP27_RS07290 WP_188212066.1 1553539..1555218(+) (pilF) [Neisseria sp. RH3002v2g]
MSVGLLRVLVQSQTISNQQAEHYNSLLQSGKEILPDLFSDGIITPKALGELVARVFSYPLLDLHYYPRNNVVGDILTEEQ
MVQNRCVPIFKRGRKVYFAVSDPTQIQNFQKIAFSSGLSVDLVVVPDDQLSSLLEWLGQRSTTILKEINEEHEATQQSQS
LYIDNEEAEDGPIPRFIHKTLSDALNAGASDIHFEFYEQMARVRFRVDGQLREVVQPPVAVRGQLASRIKVMARLDISEK
RIPQDGRIQIAFHKHGRPIDFRVSTLPTLFGEKVVMRILNSDGTSLNIDQLGLEPFQKEMLLEAINRPYGMVLVTGPTGS
GKTVSLYTCLSILNTEDVNISTAEDPAEINLPGVNQVNVNEKQGLTFATALRSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRLLNMGVAPFNIASSVSLIMAQRLLRRLCPNCKREVERPPVPALKKAGFTDADLAQDWKL
YRPVGCDSCRGKGFKGRVGIYEVMPVSDEMQKVIMNNGTEVDIMNMAYQEGMVDLRRAGLMKVMQGLTSLEEVTAHTND

Nucleotide


Download         Length: 1680 bp        

>NTDB_id=756008 BBP27_RS07290 WP_188212066.1 1553539..1555218(+) (pilF) [Neisseria sp. RH3002v2g]
ATGAGTGTCGGATTATTGCGTGTTTTGGTTCAAAGCCAAACCATCTCAAATCAACAAGCGGAACATTACAACAGCCTTTT
ACAATCGGGCAAAGAAATCCTGCCCGACTTGTTTTCAGACGGCATCATCACCCCTAAAGCCTTGGGCGAATTGGTTGCGC
GCGTATTCAGCTATCCCCTTTTGGATTTGCACTACTATCCGCGCAATAATGTAGTCGGCGACATTCTGACGGAAGAGCAA
ATGGTGCAAAACCGTTGCGTTCCGATTTTCAAACGCGGACGCAAGGTTTATTTCGCCGTATCCGATCCGACTCAAATCCA
AAACTTCCAAAAAATCGCTTTTTCTTCAGGTTTAAGCGTTGATTTGGTGGTAGTTCCCGATGACCAACTCAGCTCCTTGC
TGGAATGGTTGGGTCAGCGTTCGACCACCATTCTGAAAGAAATCAACGAAGAACATGAAGCAACGCAGCAATCTCAGTCG
CTTTATATCGATAATGAAGAGGCTGAAGACGGCCCTATCCCCCGCTTCATCCACAAAACCTTATCCGACGCCCTAAACGC
AGGCGCATCGGACATCCACTTTGAATTCTACGAGCAAATGGCGCGCGTCCGTTTCCGCGTGGACGGACAGTTGCGTGAAG
TCGTACAGCCGCCTGTCGCCGTGCGCGGCCAGTTGGCATCGCGCATCAAAGTGATGGCGCGTTTGGATATTTCTGAAAAA
CGTATTCCGCAAGACGGCAGAATTCAAATTGCCTTCCACAAACACGGCCGTCCTATTGACTTCCGTGTCAGCACTTTGCC
GACGCTGTTTGGCGAAAAAGTGGTGATGCGTATCCTAAATTCCGATGGTACATCGCTCAATATCGACCAGCTCGGTTTAG
AGCCTTTCCAAAAAGAAATGCTGCTTGAGGCCATCAACCGCCCTTACGGCATGGTGTTGGTTACCGGCCCGACAGGCTCG
GGTAAAACCGTTTCGCTCTATACCTGCCTAAGTATTTTGAACACGGAAGATGTCAACATTTCAACAGCAGAGGATCCGGC
CGAGATTAACCTGCCGGGCGTCAACCAAGTCAACGTCAATGAAAAACAAGGCCTGACGTTTGCCACCGCACTCCGATCCT
TCTTGCGTCAAGATCCTGATATTATTATGGTCGGTGAGATCCGCGACTTGGAAACCGCCGACATCGCCATTAAAGCCGCC
CAAACAGGCCACATGGTTTTCTCTACCCTGCATACCAATAATGCACCGGCCACGCTGTCCCGCCTTTTGAACATGGGTGT
CGCGCCTTTCAACATCGCCAGCTCGGTCAGCCTGATTATGGCGCAAAGGCTGTTGCGCAGACTGTGTCCAAACTGCAAAC
GCGAAGTGGAGCGCCCGCCGGTTCCGGCATTGAAAAAAGCCGGCTTTACCGATGCGGACTTGGCTCAAGATTGGAAACTC
TACCGCCCGGTTGGTTGCGACAGCTGCCGTGGCAAAGGCTTTAAAGGCCGCGTCGGTATTTATGAAGTCATGCCGGTCAG
CGATGAAATGCAAAAAGTCATTATGAACAACGGTACAGAAGTTGATATCATGAACATGGCCTATCAGGAAGGCATGGTAG
ACCTGCGTCGCGCCGGCCTCATGAAAGTCATGCAAGGCCTGACTTCGTTGGAAGAAGTAACTGCTCATACCAATGACTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-139)

Type II/IV secretion system protein (T2SSE)

(179-447)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

77.102

100

0.771
  pilB Acinetobacter baylyi ADP1

50.524

100

0.517
  pilB Acinetobacter baumannii D1279779

50.265

100

0.508
  pilB Legionella pneumophila strain ERS1305867

47.35

100

0.479
  pilB Vibrio cholerae strain A1552

45.907

100

0.462
  pilB Vibrio campbellii strain DS40M4

44.425

100

0.449
  pilB Vibrio parahaemolyticus RIMD 2210633

46.542

95.707

0.445
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.364

98.39

0.377
  pilF Thermus thermophilus HB27

38.447

96.78

0.372