Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   KI235_RS02300 Genome accession   NZ_AP021900
Coordinates   522037..524124 (+) Length   695 a.a.
NCBI ID   WP_212795469.1    Uniprot ID   -
Organism   Pseudomonas sp. St386     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 517037..529124
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI235_RS02280 (PHLH3_04430) comM 519249..520313 (+) 1065 WP_003196973.1 pilus assembly protein PilM Machinery gene
  KI235_RS02285 (PHLH3_04440) - 520313..520879 (+) 567 WP_003196975.1 PilN domain-containing protein -
  KI235_RS02290 (PHLH3_04450) pilO 520876..521499 (+) 624 WP_003196977.1 type 4a pilus biogenesis protein PilO -
  KI235_RS02295 (PHLH3_04460) - 521496..522023 (+) 528 WP_003196979.1 pilus assembly protein PilP -
  KI235_RS02300 (PHLH3_04470) pilQ 522037..524124 (+) 2088 WP_212795469.1 type IV pilus secretin PilQ Machinery gene
  KI235_RS02305 (PHLH3_04480) aroK 524129..524647 (+) 519 WP_003196983.1 shikimate kinase AroK -
  KI235_RS02310 (PHLH3_04490) aroB 524796..525896 (+) 1101 WP_003196985.1 3-dehydroquinate synthase -
  KI235_RS02315 (PHLH3_04500) - 525906..527492 (+) 1587 WP_212795470.1 AAA family ATPase -

Sequence

Protein


Download         Length: 695 a.a.        Molecular weight: 74955.99 Da        Isoelectric Point: 5.2961

>NTDB_id=75588 KI235_RS02300 WP_212795469.1 522037..524124(+) (pilQ) [Pseudomonas sp. St386]
MNRFFSALGISLWIALLSPMVLGASLKTLDVAALPGDRVELKLSFDGPPPSPRGYTTEQPARIALDLPGVTNQLSSKRRD
LGGGNARSATVVEAGDRTRLIIGLTQLAPYDTRIEGNNLFVVVGKGAAPKSTAPKSSAQAPRPAVASAAPARASAPVGKA
IRGVDFQRGTQGEGNVVIDLSDPSIAPDIQEREGKIILNFARTQLPEPLRVRLDVKDFATPVQFVNASAGSDRATISIEP
SGTFDYSTYQTDNKLTVSVRPMTVDDLQKRNADRAAYSGEKLSLNFQDIEVRSVLQLIADFTNLNLVASDTVQGGITLRL
QNVPWDQALDLVLKTKGLDKRKVGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKATDIAKLFQSVTSA
EAKADERGTITVDERTNNIIAYQTQDRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSVQNKGNWSSSG
VTTGDSTTVGTPGSTSTNQPFVDLGASGNTSGIGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGTE
IPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVNDVPPIKKNEVNAKVLVNDGETIVIGGV
FSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSDSKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2088 bp        

>NTDB_id=75588 KI235_RS02300 WP_212795469.1 522037..524124(+) (pilQ) [Pseudomonas sp. St386]
ATGAACAGGTTTTTTTCAGCCCTCGGTATTTCGCTATGGATAGCGCTGTTGTCGCCGATGGTTCTCGGGGCCAGCCTGAA
GACGTTGGATGTGGCGGCGTTGCCGGGGGACCGTGTCGAGTTGAAGCTCTCCTTCGACGGGCCGCCGCCGTCACCCCGGG
GCTACACCACCGAACAACCGGCGCGGATTGCGCTGGACCTGCCGGGCGTGACCAATCAACTTTCCAGCAAGCGCCGCGAC
CTGGGCGGGGGCAATGCACGCAGCGCCACTGTGGTCGAGGCTGGGGATCGTACTCGGTTGATCATCGGCCTTACCCAGCT
GGCGCCGTATGACACCCGGATCGAAGGTAACAACCTGTTCGTGGTGGTTGGCAAGGGCGCCGCCCCGAAGAGCACTGCCC
CGAAGAGCTCCGCCCAGGCCCCGCGCCCGGCGGTTGCCAGCGCTGCGCCTGCGAGGGCCAGTGCGCCGGTGGGCAAGGCG
ATCCGTGGGGTGGACTTCCAGCGCGGGACCCAGGGCGAAGGCAACGTGGTGATCGACCTGTCTGATCCGTCCATTGCCCC
GGACATCCAGGAGCGCGAAGGCAAGATCATCCTCAATTTCGCCAGGACCCAGTTGCCCGAGCCGCTGCGGGTGCGCCTGG
ACGTCAAGGACTTCGCCACCCCGGTGCAATTCGTCAATGCCAGCGCCGGCAGCGACCGGGCGACCATCAGCATCGAACCC
AGCGGCACCTTCGACTACTCGACCTACCAGACCGACAACAAGCTCACCGTCAGCGTCCGGCCCATGACCGTCGACGACCT
GCAAAAACGCAACGCCGACCGCGCGGCCTACAGTGGCGAGAAGCTCTCGCTCAACTTCCAGGACATCGAGGTGCGTTCGG
TCCTGCAACTGATTGCCGATTTTACCAACCTGAACCTCGTGGCCAGCGACACGGTGCAGGGTGGCATCACCCTGCGGTTG
CAGAATGTGCCCTGGGACCAGGCGCTCGACCTGGTGCTCAAGACCAAGGGGTTGGACAAGCGCAAGGTCGGCAACGTGCT
GTTGGTGGCTCCGGCCGATGAAATTGCCGCTCGTGAGCGCCAGGAACTGGAGTCGCAGAAGCAGATTGCCGACCTGGCGC
CGCTGCGGCGTGAGCTGTTGCAGGTCAACTATGCGAAGGCGACGGACATCGCCAAGCTGTTCCAGTCGGTGACCAGTGCC
GAGGCCAAAGCCGATGAGCGCGGCACGATCACGGTCGATGAGCGGACCAACAACATCATTGCCTACCAGACCCAGGACCG
TCTCGACGAACTGCGCCGGATCGTGGCGCAACTGGATATTCCGGTGCGCCAGGTGATGATCGAGGCGCGGATTGTCGAAG
CCAACGTCGACTACGACAAAAGCCTGGGTGTGCGCTGGGGCGGTTCGGTTCAAAACAAGGGCAACTGGAGCTCCTCGGGG
GTTACCACGGGCGATTCCACCACCGTTGGCACGCCGGGTAGCACCAGCACCAACCAGCCGTTCGTCGACCTGGGCGCCTC
GGGCAATACCTCGGGCATCGGCATTGCCTTCATCACCGACAACGTATTGCTGGACCTTGAGCTGACGGCCATGGAGAAAA
CCGGCAACGGGGAGATCGTCTCCCAGCCCAAGGTGGTGACGTCCGACAAGGAGACGGCAAAGATCCTCAAGGGCACGGAA
ATTCCTTATCAGGAAGCCAGTTCCAGCGGCGCTACGTCGGTGTCGTTCAAGGAGGCTTCGCTGTCGCTGGAGGTGACCCC
GCAGATCACTCCAGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGATGAGCCGGACTACCTGAACAAGGTGAATG
ATGTACCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAATGATGGCGAGACCATTGTGATTGGCGGGGTT
TTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTTGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCG
TGATGTGGTTTCGGATAGCAAATCCGAGCTGCTGGTGTTCCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGA
GTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(530-686)

Bacterial type II/III secretion system short domain (Secretin_N)

(379-444)

Secretin and TonB N terminus short domain (STN)

(305-351)

AMIN domain (AMIN)

(37-121)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.464

100

0.757
  pilQ Acinetobacter baumannii strain A118

38.627

100

0.413
  pilQ Acinetobacter baumannii D1279779

38.627

100

0.413
  comQ Acinetobacter baylyi ADP1

40.863

100

0.409
  pilQ Legionella pneumophila strain Lp02

37.941

97.842

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.722

97.266

0.367

Multiple sequence alignment