Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   KI235_RS02280 Genome accession   NZ_AP021900
Coordinates   519249..520313 (+) Length   354 a.a.
NCBI ID   WP_003196973.1    Uniprot ID   A0A0Q7AAJ5
Organism   Pseudomonas sp. St386     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 514249..525313
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  KI235_RS02270 (PHLH3_04410) - 515082..516350 (+) 1269 WP_003196970.1 malic enzyme-like NAD(P)-binding protein -
  KI235_RS02275 (PHLH3_04420) - 516596..519043 (-) 2448 WP_013692091.1 penicillin-binding protein 1A -
  KI235_RS02280 (PHLH3_04430) comM 519249..520313 (+) 1065 WP_003196973.1 pilus assembly protein PilM Machinery gene
  KI235_RS02285 (PHLH3_04440) - 520313..520879 (+) 567 WP_003196975.1 PilN domain-containing protein -
  KI235_RS02290 (PHLH3_04450) pilO 520876..521499 (+) 624 WP_003196977.1 type 4a pilus biogenesis protein PilO -
  KI235_RS02295 (PHLH3_04460) - 521496..522023 (+) 528 WP_003196979.1 pilus assembly protein PilP -
  KI235_RS02300 (PHLH3_04470) pilQ 522037..524124 (+) 2088 WP_212795469.1 type IV pilus secretin PilQ Machinery gene
  KI235_RS02305 (PHLH3_04480) aroK 524129..524647 (+) 519 WP_003196983.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38084.64 Da        Isoelectric Point: 4.9070

>NTDB_id=75587 KI235_RS02280 WP_003196973.1 519249..520313(+) (comM) [Pseudomonas sp. St386]
MLRLFNKKAHALLGIDISSTSVKLLELSRQGDRYRVESYAVEPLPANAVVEKNIAELEGVGQALSRLLAKAKTPLRSVAV
AVAGSAVITKTIEMDAGLSDDDMENQLKIEADQYIPYPLDEVAIDFEVMGVSPRSAERVEVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERAFGLLATQLAASQERLTVAVVDIGATMTTLSVLHNGRIIYTREQLFGGRQLTEEIQRRYGLTP
EQAGQAKKQGGLPDDYLSEVLQPFREALVQQVSRSLQFFFASGQYSAVDHILLAGGTASVAGLDRLIEQRLGTPTQVANP
FTNMALSSKVNAGALAGDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=75587 KI235_RS02280 WP_003196973.1 519249..520313(+) (comM) [Pseudomonas sp. St386]
GTGCTACGACTCTTCAATAAAAAAGCCCATGCGCTTCTGGGGATCGACATCAGTTCGACGTCGGTGAAGCTGCTGGAGTT
GAGCCGCCAGGGCGATCGATACCGCGTCGAGTCCTACGCGGTCGAACCGTTGCCAGCCAACGCTGTGGTCGAGAAGAACA
TCGCCGAGCTCGAAGGGGTGGGGCAGGCGTTGTCGCGCCTGTTGGCCAAGGCCAAGACGCCCTTGCGTAGCGTGGCGGTG
GCGGTGGCGGGTTCAGCGGTGATCACCAAGACCATCGAGATGGACGCCGGCCTGTCCGACGATGACATGGAAAACCAGCT
CAAGATCGAGGCCGATCAATACATCCCCTATCCCCTGGATGAGGTGGCCATCGATTTCGAAGTGATGGGTGTTTCGCCAC
GCAGTGCCGAGCGCGTCGAAGTGCTGCTGGCGGCGTGCCGCAAGGAAAACGTCGAGGTCCGCGAGGCCGCATTGGCGCTC
GCCGGGCTGACGGCCCGGGTGGTCGATGTGGAGGCTTATGCGCTGGAGCGTGCTTTTGGCCTGCTCGCCACGCAACTGGC
TGCCTCCCAGGAACGGCTGACTGTGGCGGTCGTCGATATCGGCGCCACCATGACCACCCTCAGCGTGCTGCACAACGGGC
GCATCATCTATACCCGCGAACAATTGTTCGGCGGCCGCCAACTGACCGAGGAGATCCAGCGCCGCTATGGCCTGACACCC
GAGCAGGCTGGCCAGGCAAAGAAGCAGGGTGGCCTGCCGGACGACTATCTCAGCGAAGTGCTGCAACCTTTTCGCGAGGC
CCTGGTACAGCAGGTCTCGCGGTCCTTGCAGTTCTTCTTCGCCTCGGGCCAGTACAGTGCGGTGGACCACATTCTGCTGG
CCGGGGGCACGGCTTCGGTCGCCGGCCTGGATCGGCTGATCGAGCAGCGCCTGGGCACGCCGACCCAGGTGGCCAACCCG
TTTACCAACATGGCCCTGAGCAGCAAGGTCAATGCCGGCGCCCTGGCCGGTGATGCGCCGGCACTGATGATTGCCTGTGG
GCTGGCCCTCAGGAGTTTTGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A0Q7AAJ5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

55.932

100

0.559
  pilM Acinetobacter baumannii D1279779

55.932

100

0.559
  comM Acinetobacter baylyi ADP1

54.802

100

0.548
  pilM Legionella pneumophila strain ERS1305867

47.458

100

0.475

Multiple sequence alignment