Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OKW98_RS27195 Genome accession   NZ_CP110644
Coordinates   6155221..6157299 (-) Length   692 a.a.
NCBI ID   WP_265387447.1    Uniprot ID   -
Organism   Pseudomonas sp. KU26590     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6150221..6162299
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OKW98_RS27180 (OKW98_27185) - 6151834..6153453 (-) 1620 WP_265387445.1 SPOR domain-containing protein -
  OKW98_RS27185 (OKW98_27190) aroB 6153464..6154567 (-) 1104 WP_265387446.1 3-dehydroquinate synthase -
  OKW98_RS27190 (OKW98_27195) aroK 6154698..6155216 (-) 519 WP_065986620.1 shikimate kinase AroK -
  OKW98_RS27195 (OKW98_27200) pilQ 6155221..6157299 (-) 2079 WP_265387447.1 type IV pilus secretin PilQ Machinery gene
  OKW98_RS27200 (OKW98_27205) - 6157353..6157880 (-) 528 WP_265387448.1 pilus assembly protein PilP -
  OKW98_RS27205 (OKW98_27210) pilO 6157877..6158524 (-) 648 WP_265387449.1 type 4a pilus biogenesis protein PilO -
  OKW98_RS27210 (OKW98_27215) - 6158521..6159087 (-) 567 WP_265387450.1 PilN domain-containing protein -
  OKW98_RS27215 (OKW98_27220) pilM 6159087..6160151 (-) 1065 WP_265387451.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 692 a.a.        Molecular weight: 74317.33 Da        Isoelectric Point: 5.1110

>NTDB_id=755607 OKW98_RS27195 WP_265387447.1 6155221..6157299(-) (pilQ) [Pseudomonas sp. KU26590]
MNRILSIVGLSLGMAMLSPVVQAANLKTLDVAALPGDRVELKLSFDGPVAAPRGYTTEQPARIALDLPGVTSQLAIKSRD
LGSGNAHSVVVVEAKDRTRVIINLTTLAPYSSRVDGNNLFVVVGQGAAAAQGSQPSTAMGAARVSVPAAQSSKPVARSYA
PGPRSVKNVDFQRGELGEGNVIIELSDTGIAPDIQEQGGKIRVDFAKTQLPEPLRVRLDVKDFATPVQFVNSSATGDKAS
ISIEPSGAFDYSAYQTDNKLTISVRPLTNEDQERRNSEKLVYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGG
ITLRLQNVPWDQALDLVLKTKGLDKRKIGNVLLVGPADEIAARERQELESLKQIAELAPLRRELLQVNYAKAADIAKLFQ
SVTSAESKTDERGSITVDERTNNIIAYQTQDRLDELRRIVSQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSSSKGNF
TTGGSGTVTGASATNGPYVDMGVTDATSSIGLGFLTNNTILDLELTAMEKSGNGEVVSQPKVVTSDKETAKILKGTEVPY
QEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYVNTVLGVPPIKKNEVNAKVLVADGETIVIGGVFSN
TQSKVVDKVPFLGDVPYVGRLFRRDVVSESKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2079 bp        

>NTDB_id=755607 OKW98_RS27195 WP_265387447.1 6155221..6157299(-) (pilQ) [Pseudomonas sp. KU26590]
ATGAACAGGATTCTTTCGATTGTCGGCCTGTCGCTAGGGATGGCGATGCTTTCACCGGTTGTCCAGGCGGCTAACCTCAA
GACGCTGGATGTTGCCGCATTACCGGGGGATCGAGTCGAATTGAAACTGTCCTTCGACGGTCCGGTGGCGGCGCCGCGCG
GTTACACCACCGAGCAACCGGCGCGGATTGCGCTGGACCTGCCGGGTGTGACCAGCCAGTTGGCGATCAAGAGCCGTGAC
TTGGGGTCAGGCAACGCCCACAGCGTTGTGGTCGTTGAAGCCAAGGATCGTACGCGGGTGATCATCAACCTGACCACGCT
CGCGCCGTACAGCTCGCGTGTCGACGGCAACAACCTGTTCGTGGTCGTGGGCCAGGGCGCTGCGGCGGCCCAGGGTTCAC
AGCCGAGCACCGCCATGGGCGCGGCGCGAGTGAGTGTGCCGGCCGCGCAATCGTCAAAACCTGTTGCGCGCTCCTATGCA
CCTGGCCCGCGGTCGGTAAAAAACGTCGACTTCCAGCGCGGCGAGTTGGGTGAAGGCAACGTCATCATCGAACTGAGCGA
CACCGGCATCGCGCCGGACATCCAGGAGCAGGGCGGCAAGATTCGTGTCGACTTCGCCAAAACCCAGTTGCCTGAACCCC
TGCGCGTGCGTCTGGACGTCAAGGATTTCGCGACCCCGGTGCAGTTCGTCAATTCCAGCGCCACGGGTGACAAGGCCAGT
ATTTCCATCGAACCTTCCGGTGCGTTCGACTACTCGGCCTATCAAACTGATAACAAGCTGACCATCAGCGTGCGTCCCCT
GACTAACGAAGATCAGGAGCGCCGTAACTCGGAAAAACTGGTCTACACCGGCGAGAAGCTTTCGCTGAATTTCCAGGACA
TCGACGTGCGTTCGGTGCTGCAACTGATCGCTGATTTCACCAACCTCAATCTGGTGGCGAGCGACACCGTTCAAGGCGGC
ATCACCTTGCGCCTGCAGAACGTGCCGTGGGATCAGGCGCTGGATCTGGTGCTCAAGACCAAGGGCCTGGACAAGCGCAA
GATCGGTAACGTTTTGCTGGTCGGTCCGGCTGACGAGATCGCCGCGCGGGAGCGCCAGGAGCTGGAATCACTGAAGCAGA
TTGCCGAGCTGGCACCGTTGCGTCGTGAGTTGCTGCAAGTGAACTACGCCAAGGCCGCCGACATCGCCAAGCTGTTTCAG
TCGGTGACCAGCGCTGAATCGAAAACCGACGAGCGCGGCTCGATCACCGTCGATGAGCGCACCAATAACATCATTGCCTA
CCAGACCCAGGATCGCCTGGACGAGCTGCGCCGCATCGTGTCGCAACTGGACATCCCGGTGCGCCAAGTGATGATCGAGG
CGCGCATCGTCGAGGCCAACGTCGATTACGACAAGTCACTGGGCGTGCGCTGGGGCGGTTCGAGCAGCAAGGGCAATTTC
ACTACCGGCGGCAGTGGCACTGTGACAGGCGCCTCGGCGACCAACGGTCCCTACGTAGACATGGGCGTGACTGACGCCAC
CTCGTCCATCGGCCTGGGCTTCCTGACCAACAATACGATTCTCGATCTTGAGCTGACGGCCATGGAGAAGAGCGGCAACG
GCGAAGTGGTCTCGCAGCCAAAGGTCGTCACGTCGGACAAGGAAACCGCGAAAATTCTGAAAGGTACGGAAGTGCCTTAT
CAGGAGGCGAGTTCCAGCGGCGCGACGTCGGTGTCGTTCAAAGAGGCGTCGCTGTCGCTGGAAGTCACCCCGCAGATCAC
GCCTGACAATCGCATCATCATGGAGGTCAAGGTCACGAAAGATGAGCCGGACTACGTGAACACCGTGCTGGGTGTACCGC
CGATCAAGAAAAACGAAGTCAACGCCAAGGTGCTGGTGGCCGACGGCGAGACCATCGTAATCGGTGGCGTGTTCTCGAAT
ACGCAGAGTAAAGTCGTCGACAAGGTGCCATTTTTGGGCGATGTGCCGTATGTTGGCCGCCTTTTCCGGCGCGATGTGGT
TTCGGAGTCAAAATCCGAGCTGTTGGTGTTTCTCACTCCGCGTATCATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(527-683)

AMIN domain (AMIN)

(38-122)

Secretin and TonB N terminus short domain (STN)

(310-356)

Bacterial type II/III secretion system short domain (Secretin_N)

(384-449)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

72.841

100

0.756
  comQ Acinetobacter baylyi ADP1

42.672

100

0.429
  pilQ Acinetobacter baumannii strain A118

38.723

100

0.412
  pilQ Acinetobacter baumannii D1279779

38.723

100

0.412
  pilQ Legionella pneumophila strain Lp02

39.562

98.988

0.392
  pilQ Legionella pneumophila strain ERS1305867

39.385

98.699

0.389