Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OM794_RS09855 Genome accession   NZ_CP110535
Coordinates   2163334..2165091 (-) Length   585 a.a.
NCBI ID   WP_226249424.1    Uniprot ID   -
Organism   Halomonas sp. BDJS001     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2158334..2170091
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OM794_RS09825 (OM794_09825) - 2158940..2159281 (+) 342 WP_226249429.1 hypothetical protein -
  OM794_RS09830 (OM794_09830) - 2159324..2160211 (-) 888 WP_226249428.1 DNA replication terminus site-binding protein -
  OM794_RS09835 (OM794_09835) yacG 2160251..2160484 (-) 234 WP_022523595.1 DNA gyrase inhibitor YacG -
  OM794_RS09840 (OM794_09840) coaE 2160519..2161121 (-) 603 WP_226249427.1 dephospho-CoA kinase -
  OM794_RS09845 (OM794_09845) pilD 2161204..2162049 (-) 846 WP_226249426.1 prepilin peptidase Machinery gene
  OM794_RS09850 (OM794_09850) pilC 2162098..2163330 (-) 1233 WP_226249425.1 type II secretion system F family protein Machinery gene
  OM794_RS09855 (OM794_09855) pilB 2163334..2165091 (-) 1758 WP_226249424.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OM794_RS09865 (OM794_09865) - 2165333..2165812 (+) 480 WP_319001073.1 pilin -
  OM794_RS09870 (OM794_09870) - 2165929..2166363 (+) 435 WP_226249423.1 anti-virulence regulator CigR family protein -
  OM794_RS09875 (OM794_09875) - 2166458..2167279 (+) 822 WP_226249422.1 inositol monophosphatase family protein -
  OM794_RS09885 (OM794_09885) - 2167893..2168522 (-) 630 WP_226249421.1 LysE family translocator -
  OM794_RS09890 (OM794_09890) - 2168653..2169471 (-) 819 WP_226249610.1 alpha/beta fold hydrolase -
  OM794_RS09895 (OM794_09895) - 2169635..2170063 (-) 429 WP_226249420.1 organic hydroperoxide resistance protein -

Sequence

Protein


Download         Length: 585 a.a.        Molecular weight: 64090.48 Da        Isoelectric Point: 6.5620

>NTDB_id=755265 OM794_RS09855 WP_226249424.1 2163334..2165091(-) (pilB) [Halomonas sp. BDJS001]
MSQPPFESTLSHAAARGGLRGIAQRLVKHGLLTDAQAATAETTASNLGISLLQHVIESGLVDPNKATLAAGWEYGLPVID
LEAVRLSALPPASDYPIKVLQSLNVLPLARHGHRLTVAVPYPATLTQLDELQFATGLSVEGILAPVDQITVALNSYLAQN
GRSMLDQLDELDDAVNSLSVVQNNDGDEVPLEESSQNSEDAPIVKFVNKILLDAIRRGASDIHFEPYETLYRVRFRIDGI
LIEVARPPFAMRNRIAARLKIMARLDISERRLPQDGSIKLKLSRTRSLDFRVNSLPTVYGEKLVLRILDPAAAQLGIEQL
GFTDEQRGHYEHALKQPQGMILVTGPTGSGKTVSLYTGINILNKVERNICTAEDPVEIKVAGINQVNVLPKIGLDFASAL
RAFLRQDPDVVMVGEIRDLETAEIAVKAAQTGHLVLSTVHTNSAAETLTRLANMGVSSFNIASAVSLIIAQRLARKLCQH
CKEPIDIPHEALRKEGFSSEEIHQATIYRSVGCKQCTHGYKGRIGIYEVVPITEAMRQLIMRDANALDIDQQRREEGYPS
LHRSGLLKVMQGITSLEEVNRVSKE

Nucleotide


Download         Length: 1758 bp        

>NTDB_id=755265 OM794_RS09855 WP_226249424.1 2163334..2165091(-) (pilB) [Halomonas sp. BDJS001]
ATGAGCCAGCCCCCTTTTGAGTCGACGTTAAGCCACGCCGCCGCGCGTGGTGGCCTGCGCGGCATCGCCCAGCGCTTGGT
TAAGCACGGCTTGTTGACCGATGCCCAAGCCGCCACGGCGGAAACCACCGCGTCGAACCTGGGAATATCGTTACTTCAGC
ATGTGATCGAAAGTGGCCTGGTCGACCCTAACAAAGCGACACTGGCGGCTGGCTGGGAGTATGGGCTGCCGGTCATAGAT
CTAGAAGCAGTTCGTCTGTCCGCGCTGCCTCCCGCTTCTGACTACCCTATCAAAGTGCTGCAAAGCCTTAACGTTCTGCC
ATTAGCCCGTCATGGCCATCGCCTAACGGTTGCGGTTCCCTACCCCGCCACTTTGACGCAACTGGATGAGTTGCAATTTG
CGACCGGCCTGAGTGTGGAAGGTATCCTCGCTCCCGTTGATCAAATCACAGTCGCACTCAATAGCTACCTTGCTCAAAAC
GGGCGTAGCATGCTTGATCAGCTGGATGAGTTAGATGATGCGGTGAATTCACTGAGCGTTGTGCAGAACAACGATGGTGA
CGAAGTTCCTCTGGAAGAGTCCTCTCAAAATAGTGAAGACGCGCCTATTGTTAAGTTCGTCAACAAAATACTCTTAGACG
CGATTCGCCGGGGCGCCTCTGATATCCATTTTGAGCCTTACGAAACCCTCTACCGGGTACGCTTTCGTATTGATGGAATT
CTCATAGAAGTCGCGCGACCACCCTTTGCCATGCGCAATCGCATCGCAGCCCGCCTAAAAATTATGGCCCGACTGGATAT
ATCGGAACGTCGCTTACCCCAGGATGGCTCGATAAAGTTAAAGCTTTCACGCACCCGCTCTCTCGATTTCCGGGTTAACT
CACTGCCAACAGTCTATGGCGAAAAGTTAGTGCTGCGAATATTAGATCCGGCCGCAGCGCAACTGGGTATTGAGCAACTG
GGTTTTACCGACGAACAACGGGGCCATTATGAACATGCGTTAAAACAACCCCAGGGCATGATTTTGGTCACCGGTCCTAC
CGGCAGTGGTAAAACTGTTTCGTTATATACCGGCATTAATATCCTTAATAAAGTGGAACGTAATATTTGTACCGCTGAAG
ATCCGGTTGAGATCAAAGTCGCCGGTATCAATCAAGTGAACGTATTACCTAAGATCGGCCTCGATTTTGCCAGTGCACTA
AGGGCGTTTTTACGCCAAGACCCGGATGTGGTCATGGTAGGCGAAATTCGTGATCTTGAAACCGCCGAAATTGCCGTTAA
AGCAGCCCAGACAGGCCACCTGGTGCTCTCTACGGTGCATACCAACTCGGCAGCCGAAACGCTAACTCGACTGGCGAATA
TGGGCGTCTCTTCGTTTAACATCGCCAGCGCCGTTAGTTTGATTATTGCCCAGCGGCTGGCCCGCAAACTGTGTCAACAC
TGCAAAGAGCCCATTGACATTCCCCATGAGGCACTGCGCAAAGAGGGCTTTAGCAGCGAAGAAATCCATCAGGCGACTAT
CTACAGGTCGGTAGGTTGCAAGCAGTGTACCCACGGCTATAAAGGGCGAATTGGCATTTATGAGGTAGTGCCGATTACCG
AAGCGATGCGTCAGTTGATTATGCGCGATGCTAACGCATTAGATATCGATCAGCAGAGGCGCGAGGAGGGCTACCCAAGC
CTGCACCGCAGTGGGCTACTGAAAGTCATGCAGGGTATTACCAGCCTGGAAGAGGTTAACCGAGTGAGCAAGGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(56-159)

Type II/IV secretion system protein (T2SSE)

(209-476)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

51.237

96.752

0.496
  pilB Acinetobacter baylyi ADP1

50.615

97.265

0.492
  pilB Acinetobacter baumannii D1279779

50.441

96.923

0.489
  pilB Vibrio campbellii strain DS40M4

49.209

97.265

0.479
  pilB Vibrio cholerae strain A1552

49.91

94.872

0.474
  pilB Vibrio parahaemolyticus RIMD 2210633

48.582

96.41

0.468
  pilF Neisseria gonorrhoeae MS11

45.423

97.094

0.441
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.372

100

0.374
  pilF Thermus thermophilus HB27

37.478

96.239

0.361