Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   ONR73_RS02115 Genome accession   NZ_CP110364
Coordinates   425092..426798 (-) Length   568 a.a.
NCBI ID   WP_005336134.1    Uniprot ID   -
Organism   Aeromonas veronii strain C4     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 420092..431798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ONR73_RS02085 (ONR73_02085) mutT 420604..421008 (+) 405 WP_042053534.1 8-oxo-dGTP diphosphatase MutT -
  ONR73_RS02090 (ONR73_02090) yacG 421086..421280 (-) 195 WP_005336142.1 DNA gyrase inhibitor YacG -
  ONR73_RS02095 (ONR73_02095) zapD 421290..422012 (-) 723 WP_005336141.1 cell division protein ZapD -
  ONR73_RS02100 (ONR73_02100) coaE 422050..422664 (-) 615 WP_265062002.1 dephospho-CoA kinase -
  ONR73_RS02105 (ONR73_02105) pilD 422678..423550 (-) 873 WP_265062003.1 A24 family peptidase Machinery gene
  ONR73_RS02110 (ONR73_02110) - 423612..424852 (-) 1241 Protein_406 type II secretion system F family protein -
  ONR73_RS02115 (ONR73_02115) pilB 425092..426798 (-) 1707 WP_005336134.1 PilB family type IVa pilus assembly ATPase TapB Machinery gene
  ONR73_RS02120 (ONR73_02120) tapA 426802..427293 (-) 492 WP_406801101.1 type IVa pilus major pilin TapA -
  ONR73_RS02125 (ONR73_02125) nadC 427608..428483 (-) 876 WP_265062005.1 carboxylating nicotinate-nucleotide diphosphorylase -
  ONR73_RS02130 (ONR73_02130) - 428487..428957 (-) 471 WP_040068932.1 TIGR02281 family clan AA aspartic protease -
  ONR73_RS02135 (ONR73_02135) ampD 429088..429663 (+) 576 WP_167571392.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  ONR73_RS02140 (ONR73_02140) pdhR 429942..430706 (+) 765 WP_005336127.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 568 a.a.        Molecular weight: 62533.87 Da        Isoelectric Point: 5.3192

>NTDB_id=754557 ONR73_RS02115 WP_005336134.1 425092..426798(-) (pilB) [Aeromonas veronii strain C4]
MTSSPNSGLALSLAASSLISEEDSQRYLSQAKAQRKPFVTFLIENEILDSKALADFCELEYGVPLLDLAAFDLAEIPQKY
LNQKLIEKHHVLPIYTQGHTLYIAMSDPTNVSALEDFGFSFGLHTEALLVEENKLTAAIGKLLESENDALGMEDIDESEI
SDLEVSDENSRLDESVNTADDDAPIVKYINKILMDAIKRGASDLHFEPYEFKYRVRFRIDGILHEIATPPVNLSNRFSAR
LKVMARLDIAERRLPQDGRIKLKLSRNKSMDMRVNTLPTMWGEKIVIRLLDSSAARLNIEQLGFDDRQKAQYLRALSKPQ
GMILVTGPTGSGKTVSLYTGLNILNTTEVNISTAEDPVEINLPGVNQVQVNPKAGLTFASALRSFLRQDPDVVMVGEIRD
LETAEIAIKAAQTGHLVLSTLHTNSAAETLTRMMNMGVPAFNIASSVTLIMAQRLARKLCDHCKAPEVVPEAELLELGFT
PQQLAAGLRLFKPVGCKECSGGYKGRVGIYEIMLMSENIAKLIMQGANSLQIAAIAQKEGMRTLRTSGLEKARLGVTSLA
EVNRVTTN

Nucleotide


Download         Length: 1707 bp        

>NTDB_id=754557 ONR73_RS02115 WP_005336134.1 425092..426798(-) (pilB) [Aeromonas veronii strain C4]
ATGACCTCTAGCCCTAACAGCGGCCTGGCGTTAAGCCTGGCCGCCTCTTCACTCATAAGTGAAGAGGATTCCCAGCGCTA
TCTGAGCCAGGCCAAGGCCCAGCGCAAACCCTTTGTGACATTTCTGATAGAGAACGAGATCCTCGACAGCAAGGCGCTCG
CCGATTTTTGCGAGCTGGAGTATGGGGTACCTCTGCTGGATCTTGCCGCCTTCGACTTGGCCGAGATCCCGCAGAAGTAC
CTCAATCAGAAGCTGATTGAGAAACACCACGTGCTGCCCATCTACACCCAGGGCCACACCCTCTATATCGCCATGTCGGA
TCCGACTAATGTCTCGGCGTTAGAGGACTTTGGCTTCAGCTTCGGCCTTCATACCGAAGCGCTGCTGGTGGAGGAGAACA
AGCTCACCGCCGCCATCGGCAAGCTGCTGGAGAGCGAAAACGACGCCCTCGGCATGGAGGATATCGACGAATCTGAAATT
TCGGATCTCGAGGTCTCCGACGAGAACAGCCGGCTGGACGAAAGTGTCAATACCGCCGATGACGATGCCCCCATCGTCAA
GTACATCAACAAGATCCTGATGGATGCCATCAAGCGCGGAGCCTCCGACCTGCACTTCGAGCCCTATGAGTTCAAGTATC
GGGTGCGCTTTCGCATCGATGGCATTCTGCATGAAATTGCCACACCGCCGGTCAACCTCTCCAACCGCTTCTCCGCTCGC
CTCAAGGTCATGGCACGACTCGATATCGCCGAGCGGCGGCTGCCACAGGATGGCCGCATCAAGCTGAAGCTGTCGCGCAA
CAAGTCGATGGATATGCGGGTCAATACCCTGCCCACCATGTGGGGGGAGAAGATCGTTATCCGTCTGCTCGACTCCTCGG
CGGCACGCCTCAATATCGAGCAACTCGGCTTTGATGACCGGCAGAAGGCGCAGTACCTGCGCGCCCTCTCCAAACCACAG
GGGATGATCCTGGTGACTGGCCCCACCGGCTCGGGCAAAACGGTCTCGCTCTATACCGGCCTCAATATTCTCAACACTAC
CGAGGTAAATATCTCTACCGCCGAAGATCCGGTGGAGATCAACTTGCCCGGCGTCAACCAGGTGCAGGTCAACCCCAAGG
CGGGGCTCACCTTCGCCAGCGCCTTGCGCTCCTTCCTGCGGCAAGATCCGGATGTGGTGATGGTGGGTGAAATTCGCGAT
CTCGAAACCGCCGAGATCGCTATCAAGGCGGCGCAGACCGGCCACCTGGTGCTCTCCACCCTGCATACCAACTCCGCCGC
CGAGACCCTGACCCGGATGATGAACATGGGGGTTCCGGCCTTCAACATCGCCTCCTCGGTGACCCTGATCATGGCCCAGC
GGTTGGCCCGTAAACTGTGCGACCACTGCAAAGCGCCGGAAGTGGTGCCGGAAGCCGAGCTGCTGGAGCTGGGCTTTACC
CCACAGCAGTTGGCAGCCGGACTGCGACTGTTCAAACCGGTCGGTTGCAAGGAGTGTTCCGGCGGCTACAAGGGGCGGGT
AGGCATCTACGAAATCATGCTGATGTCAGAGAACATCGCCAAATTGATCATGCAAGGGGCAAACTCCCTGCAAATCGCCG
CTATCGCCCAAAAGGAGGGGATGCGTACCCTGCGCACCTCGGGACTGGAGAAGGCCCGGCTCGGGGTCACCAGTCTGGCC
GAGGTCAACCGGGTCACCACCAACTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-146)

Type II/IV secretion system protein (T2SSE)

(191-458)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

55.986

100

0.56
  pilB Acinetobacter baylyi ADP1

55.789

100

0.56
  pilB Legionella pneumophila strain ERS1305867

56.642

95.423

0.54
  pilB Vibrio cholerae strain A1552

54.758

98.063

0.537
  pilB Vibrio campbellii strain DS40M4

51.22

100

0.518
  pilB Vibrio parahaemolyticus RIMD 2210633

51.596

99.296

0.512
  pilF Neisseria gonorrhoeae MS11

46.809

99.296

0.465
  pilB/pilB1 Synechocystis sp. PCC 6803

39.06

97.359

0.38
  pilF Thermus thermophilus HB27

40.53

92.958

0.377